Electron Microscopy Data Bank

EMD-11596

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Shotgun EM of Mycobacterial protein complexes during stationary phase stress

Release date: Sept. 9, 2020
Resolution: 16.0 Å
EM Method: Single-particle

Kirykowicz AM, Woodward JD

Curr Res Struct Biol (2020) 2 pp. 204-212 [ DOI: doi:10.1016/j.crstbi.2020.09.002 Pubmed: 34235480 ]

Sample:

Bacterioferritin a (440 kDa, Complex from Mycolicibacterium smegmatis MC2 155)
Bacterioferritin a (Protein from Mycolicibacterium smegmatis MC2 155)

EMD-11598

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Shotgun EM of Mycobacterial protein complexes during stationary phase stress.

Release date: Sept. 9, 2020
Resolution: 27.0 Å
EM Method: Single-particle

Kirykowicz AM, Woodward JD

Curr Res Struct Biol (2020) 2 pp. 204-212 [ Pubmed: 34235480 DOI: doi:10.1016/j.crstbi.2020.09.002 ]

Sample:

Encapsulin containing dyp peroxidase cargo protein. (1 MDa, Complex from Mycolicibacterium smegmatis MC2 155)
Encapsulin (Protein from Mycolicibacterium smegmatis MC2 155)
Dyp-peroxidase (Protein from Mycolicibacterium smegmatis MC2 155)

EMD-11599

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Shotgun EM of Mycobacterial protein complexes during stationary phase stress

Release date: Sept. 9, 2020
Resolution: 19.0 Å
EM Method: Single-particle

Kirykowicz AM, Woodward JD

Curr Res Struct Biol (2020) 2 pp. 204-212 [ DOI: doi:10.1016/j.crstbi.2020.09.002 Pubmed: 34235480 ]

Sample:

Glutamine synthetase (Protein from Mycolicibacterium smegmatis MC2 155)
Glutamine synthetase (610 kDa, Complex from Mycolicibacterium smegmatis MC2 155)

EMD-11595

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Shotgun EM of Mycobacterial protein complexes during stationary phase stress.

Release date: Aug. 19, 2020
Resolution: 26.0 Å
EM Method: Single-particle

Kirykowicz AM, Woodward JD

Curr Res Struct Biol (2020) 2 pp. 204-212 [ DOI: doi:10.1016/j.crstbi.2020.09.002 Pubmed: 34235480 ]

Sample:

Probable m18 family aminopeptidase 2 (540 kDa, Complex from Mycolicibacterium smegmatis MC2 155)
Aspartyl aminopeptidase (Protein from Mycolicibacterium smegmatis MC2 155)

EMD-11597

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Shotgun EM of Mycobacterial protein complexes during stationary phase stress.

Release date: Aug. 19, 2020
Resolution: 18.0 Å
EM Method: Single-particle

Kirykowicz AM, Woodward JD

Curr Res Struct Biol (2020) 2 pp. 204-212 [ Pubmed: 34235480 DOI: doi:10.1016/j.crstbi.2020.09.002 ]

Sample:

Encapsulin (Protein from Mycolicibacterium smegmatis MC2 155)
Encapsulin (1 MDa, Complex from Mycolicibacterium smegmatis MC2 155)

EMD-4175

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Native encapsulin from Mycobacterium smegmatis

Release date: July 22, 2020
Resolution: 25.0 Å
EM Method: Single-particle

Kirykowicz AM, Woodward JD

To Be Published

Sample:

Cfp29 (1 MDa, Complex from Mycobacterium smegmatis str. MC2 155)

EMD-10004

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Native encapsulated dye decolourising type peroxidase from Mycobacterium smegmatis

Release date: May 6, 2020
Resolution: 25.0 Å
EM Method: Single-particle

Kirykowicz AM, Woodward JD

To Be Published

Sample:

Encapsulated dyp type peroxidase (222 kDa, Complex from Mycobacterium smegmatis str. MC2 155)

EMD-10005

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Native bacterioferritin from Mycobacterium smegmatis

Release date: May 6, 2020
Resolution: 20.0 Å
EM Method: Single-particle

Kirykowicz AM, Woodward JD

To Be Published

Sample:

Bacterioferritin; ferritin family protein (480 kDa, Complex from Mycobacterium smegmatis str. MC2 155)

EMD-10008

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Native encapsulin with bound dye decolourising peroxidase on three fold axis

Release date: May 6, 2020
Resolution: 30.0 Å
EM Method: Single-particle

Kirykowicz AM, Woodward JD

To Be Published

Sample:

Encapsulin with bound dyp on three fold axis (1 MDa, Complex from Mycobacterium smegmatis str. MC2 155)

EMD-4418

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Cryo-EM informed directed evolution of Nitrilase 4 leads to a change in quaternary structure.

Release date: Nov. 20, 2019
Resolution: 3.9 Å
EM Method: Helical reconstruction
Fitted PDBs: 6i5t
Q-score: 0.472

Mulelu AE, Kirykowicz AM, Woodward JD

To Be Published

Sample:

Bifunctional nitrilase/nitrile hydratase nit4 (39 kDa, Protein from Arabidopsis thaliana)
Active helical nitrilase complex (Complex from Arabidopsis thaliana)

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