48-nm doublet microtubule from Tetrahymena thermophila strain MEC17
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 8sf7
Q-score: 0.206
Yang SK, Kubo S, Black CS, Peri K, Dai D, Legal T, Valente-Paterno M, Gaertig J, Bui KH
eLife (2024) 12 [ Pubmed: 38598282 DOI: doi:10.7554/eLife.92219 ]
- Cfap127 (55 kDa, Protein from Tetrahymena thermophila)
- Rib26 (26 kDa, Protein from Tetrahymena thermophila)
- Rib27a (27 kDa, Protein from Tetrahymena thermophila)
- Oj2 (19 kDa, Protein from Tetrahymena thermophila)
- Cfap112a (30 kDa, Protein from Tetrahymena thermophila)
- Parkin co-regulated protein pacrgb (28 kDa, Protein from Tetrahymena thermophila)
- Flagellar microtugule protofilament ribbon protein (72 kDa, Protein from Tetrahymena thermophila)
- Cfap210 (61 kDa, Protein from Tetrahymena thermophila)
- Cilia- and flagella-associated protein 52 (73 kDa, Protein from Tetrahymena thermophila)
- Cfam166c (25 kDa, Protein from Tetrahymena thermophila)
- Cfap161a (57 kDa, Protein from Tetrahymena thermophila)
- Cfap182a (20 kDa, Protein from Tetrahymena thermophila)
- Cfap21a (48 kDa, Protein from Tetrahymena thermophila)
- Cfap129 (31 kDa, Protein from Tetrahymena thermophila)
- Cfap20 (22 kDa, Protein from Tetrahymena thermophila)
- 48-nm repeat unit of microtubule doublet from tetrahymena thermophila strain mec17 (7 MDa, Cellular component from Tetrahymena thermophila)
- Rib57 (57 kDa, Protein from Tetrahymena thermophila)
- Cfap115 (110 kDa, Protein from Tetrahymena thermophila)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Cilia- and flagella-associated protein 45 (59 kDa, Protein from Tetrahymena thermophila)
- Cfap143 (30 kDa, Protein from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Tetrahymena thermophila)
- Parkin co-regulated protein pacrga (36 kDa, Protein from Tetrahymena thermophila)
- Protofilament ribbon protein (34 kDa, Protein from Tetrahymena thermophila)
- Nebulin (61 kDa, Protein from Tetrahymena thermophila)
- Stpg2 (33 kDa, Protein from Tetrahymena thermophila)
- Cfap112b (32 kDa, Protein from Tetrahymena thermophila)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Rib43a protein (17 kDa, Protein from Tetrahymena thermophila)
- Dna polymerase delta c4-type zinc-finger protein (31 kDa, Protein from Tetrahymena thermophila)
- Cfap107 (21 kDa, Protein from Tetrahymena thermophila)
- Cilia- and flagella-associated protein 53 (60 kDa, Protein from Tetrahymena thermophila)
- Rib27b (26 kDa, Protein from Tetrahymena thermophila)
- Nucleoside diphosphate kinase (42 kDa, Protein from Tetrahymena thermophila)
- Flagellar flij protein (12 kDa, Protein from Tetrahymena thermophila)
- Sb1 (17 kDa, Protein from Tetrahymena thermophila)
- Cfap77a (28 kDa, Protein from Tetrahymena thermophila)
- Ij34 (34 kDa, Protein from Tetrahymena thermophila)
- Oj3 (19 kDa, Protein from Tetrahymena thermophila)
- Stpg1a (42 kDa, Protein from Tetrahymena thermophila)
- Tubulin alpha chain (49 kDa, Protein from Tetrahymena thermophila)
- Cfam166a (25 kDa, Protein from Tetrahymena thermophila)
- Cfam166b (17 kDa, Protein from Tetrahymena thermophila)
- Rib22 (22 kDa, Protein from Tetrahymena thermophila)
- Rib38 (38 kDa, Protein from Tetrahymena thermophila)
- Rib72b (60 kDa, Protein from Tetrahymena thermophila)
- Cfap182b (11 kDa, Protein from Tetrahymena thermophila)
- Rib35 (34 kDa, Protein from Tetrahymena thermophila)
96-nm repeat unit of intact doublet microtubule from Tetrahymena thermophila CFAP77AB-KO mutant
Kubo S, Black CS, Joachimiak E, Yang SK, Legal T, Peri K, Khalifa AAZ, Ghanaeian A, McCafferty CL, Valente-Paterno M, De Bellis C, Huynh PM, Fan Z, Marcotte EM, Wloga D, Bui KH
Nat Commun (2023) 14 pp. 2168-2168 [ Pubmed: 37061538 DOI: doi:10.1038/s41467-023-37868-0 ]
96-nm repeat unit of intact doublet microtubule from Tetrahymena thermophila strain CU428
Kubo S, Black CS, Joachimiak E, Yang SK, Legal T, Peri K, Khalifa AAZ, Ghanaeian A, McCafferty CL, Valente-Paterno M, De Bellis C, Huynh PM, Fan Z, Marcotte EM, Wloga D, Bui KH
Nat Commun (2023) 14 pp. 2168-2168 [ Pubmed: 37061538 DOI: doi:10.1038/s41467-023-37868-0 ]
48-nm doublet microtubule from Tetrahymena thermophila strain CU428
Kubo S, Black CS, Joachimiak E, Yang SK, Legal T, Peri K, Khalifa AAZ, Ghanaeian A, McCafferty CL, Valente-Paterno M, De Bellis C, Huynh PM, Fan Z, Marcotte EM, Wloga D, Bui KH
Nat Commun (2023) 14 pp. 2168-2168 [ DOI: doi:10.1038/s41467-023-37868-0 Pubmed: 37061538 ]
- Cfap53 (60 kDa, Protein from Tetrahymena thermophila)
- B5b6_fmip (43 kDa, Protein from Tetrahymena thermophila)
- Rib26 (26 kDa, Protein from Tetrahymena thermophila)
- Cfap210 (61 kDa, Protein from Tetrahymena thermophila)
- Sb1 (17 kDa, Protein from Tetrahymena thermophila)
- Tubulin beta chain (49 kDa, Protein from Tetrahymena thermophila)
- Flagellar microtubule protofilament ribbon protein (72 kDa, Protein from Tetrahymena thermophila)
- Rib35 (34 kDa, Protein from Tetrahymena thermophila)
- Cfap182b (11 kDa, Protein from Tetrahymena thermophila)
- Stpg2 (33 kDa, Protein from Tetrahymena thermophila)
- Stpg1a (42 kDa, Protein from Tetrahymena thermophila)
- Rib72b (60 kDa, Protein from Tetrahymena thermophila)
- Rib57 (57 kDa, Protein from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Nucleoside diphosphate kinase (42 kDa, Protein from Tetrahymena thermophila)
- Rib22 (22 kDa, Protein from Tetrahymena thermophila)
- Cfap112a (45 kDa, Protein from Tetrahymena thermophila)
- Rib38 (38 kDa, Protein from Tetrahymena thermophila)
- Cfap115 (110 kDa, Protein from Tetrahymena thermophila)
- Cfap20 (22 kDa, Protein from Tetrahymena thermophila)
- Oj3 (14 kDa, Protein from Tetrahymena thermophila)
- Rib27a (27 kDa, Protein from Tetrahymena thermophila)
- Cfap77a (28 kDa, Protein from Tetrahymena thermophila)
- Cfap21a (48 kDa, Protein from Tetrahymena thermophila)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Nebulin (61 kDa, Protein from Tetrahymena thermophila)
- Cfam166b (17 kDa, Protein from Tetrahymena thermophila)
- Tubulin alpha chain (49 kDa, Protein from Tetrahymena thermophila)
- Parkin co-regulated protein pacrga (36 kDa, Protein from Tetrahymena thermophila)
- Ij34 (34 kDa, Protein from Tetrahymena thermophila)
- Parkin co-regulated protein pacrgb (28 kDa, Protein from Tetrahymena thermophila)
- 48-nm repeat unit of microtubule doublet from tetrahymena thermophila strain cu428 (7 MDa, Cellular component from Tetrahymena thermophila)
- Flagellar flij protein (12 kDa, Protein from Tetrahymena thermophila)
- Cfap129 (31 kDa, Protein from Tetrahymena thermophila)
- Cfap143 (30 kDa, Protein from Tetrahymena thermophila)
- Cfap127 (55 kDa, Protein from Tetrahymena thermophila)
- Rib27b (26 kDa, Protein from Tetrahymena thermophila)
- B2b3_fmip (31 kDa, Protein from Tetrahymena thermophila)
- Cfap182a (20 kDa, Protein from Tetrahymena thermophila)
- Cfap52a (73 kDa, Protein from Tetrahymena thermophila)
- Dna polymerase delta c4-type zinc-finger protein (31 kDa, Protein from Tetrahymena thermophila)
- Cilia- and flagella-associated protein 45 (59 kDa, Protein from Tetrahymena thermophila)
- Cfam166a (25 kDa, Protein from Tetrahymena thermophila)
- Protofilament ribbon protein (34 kDa, Protein from Tetrahymena thermophila)
- Cfap107 (21 kDa, Protein from Tetrahymena thermophila)
- Oj2 (19 kDa, Protein from Tetrahymena thermophila)
- Cfap161a (57 kDa, Protein from Tetrahymena thermophila)
- Rib43a protein (17 kDa, Protein from Tetrahymena thermophila)
- Cfam166c (25 kDa, Protein from Tetrahymena thermophila)
48-nm doublet microtubule from Tetrahymena thermophila strain K40R
Kubo S, Black CS, Joachimiak E, Yang SK, Legal T, Peri K, Khalifa AAZ, Ghanaeian A, McCafferty CL, Valente-Paterno M, De Bellis C, Huynh PM, Fan Z, Marcotte EM, Wloga D, Bui KH
Nat Commun (2023) 14 pp. 2168-2168 [ DOI: doi:10.1038/s41467-023-37868-0 Pubmed: 37061538 ]
- Cfap127 (55 kDa, Protein from Tetrahymena thermophila)
- Rib26 (26 kDa, Protein from Tetrahymena thermophila)
- Rib27a (27 kDa, Protein from Tetrahymena thermophila)
- Oj2 (19 kDa, Protein from Tetrahymena thermophila)
- Parkin co-regulated protein pacrgb (28 kDa, Protein from Tetrahymena thermophila)
- Flagellar microtugule protofilament ribbon protein (72 kDa, Protein from Tetrahymena thermophila)
- Cfap210 (61 kDa, Protein from Tetrahymena thermophila)
- Cilia- and flagella-associated protein 52 (73 kDa, Protein from Tetrahymena thermophila)
- Cfam166c (25 kDa, Protein from Tetrahymena thermophila)
- Cfap161a (57 kDa, Protein from Tetrahymena thermophila)
- Cfap182a (20 kDa, Protein from Tetrahymena thermophila)
- Cfap21a (48 kDa, Protein from Tetrahymena thermophila)
- Cfap129 (31 kDa, Protein from Tetrahymena thermophila)
- Cfap20 (22 kDa, Protein from Tetrahymena thermophila)
- Rib57 (57 kDa, Protein from Tetrahymena thermophila)
- Cfap115 (110 kDa, Protein from Tetrahymena thermophila)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Cilia- and flagella-associated protein 45 (59 kDa, Protein from Tetrahymena thermophila)
- Cfap143 (30 kDa, Protein from Tetrahymena thermophila)
- Tubulin beta chain (49 kDa, Protein from Tetrahymena thermophila)
- B2b3_fmip (31 kDa, Protein from Tetrahymena thermophila)
- Cfap112a (45 kDa, Protein from Tetrahymena thermophila)
- Parkin co-regulated protein pacrga (36 kDa, Protein from Tetrahymena thermophila)
- Protofilament ribbon protein (34 kDa, Protein from Tetrahymena thermophila)
- Stpg2 (33 kDa, Protein from Tetrahymena thermophila)
- Tubulin alpha chain (49 kDa, Protein from Tetrahymena thermophila)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Rib43a protein (17 kDa, Protein from Tetrahymena thermophila)
- Dna polymerase delta c4-type zinc-finger protein (31 kDa, Protein from Tetrahymena thermophila)
- Cfap107 (21 kDa, Protein from Tetrahymena thermophila)
- Cilia- and flagella-associated protein 53 (60 kDa, Protein from Tetrahymena thermophila)
- Rib27b (26 kDa, Protein from Tetrahymena thermophila)
- Nucleoside diphosphate kinase (42 kDa, Protein from Tetrahymena thermophila)
- 48-nm repeat unit of microtubule doublet from tetrahymena thermophila strain cu428 (7 MDa, Cellular component from Tetrahymena thermophila)
- Flagellar flij protein (12 kDa, Protein from Tetrahymena thermophila)
- Oj3 (14 kDa, Protein from Tetrahymena thermophila)
- Sb1 (17 kDa, Protein from Tetrahymena thermophila)
- Cfap77a (28 kDa, Protein from Tetrahymena thermophila)
- Ij34 (34 kDa, Protein from Tetrahymena thermophila)
- Stpg1a (42 kDa, Protein from Tetrahymena thermophila)
- Cfam166a (25 kDa, Protein from Tetrahymena thermophila)
- Nebulin (61 kDa, Protein from Tetrahymena thermophila)
- Cfam166b (17 kDa, Protein from Tetrahymena thermophila)
- Rib22 (22 kDa, Protein from Tetrahymena thermophila)
- Rib38 (38 kDa, Protein from Tetrahymena thermophila)
- Rib72b (60 kDa, Protein from Tetrahymena thermophila)
- B5b6_fmip (43 kDa, Protein from Tetrahymena thermophila)
- Cfap182b (11 kDa, Protein from Tetrahymena thermophila)
- Rib35 (34 kDa, Protein from Tetrahymena thermophila)
96-nm doublet microtubule from combined Tetrahymena thermophila strains CU428 and K40R
Kubo S, Black CS, Joachimiak E, Yang SK, Legal T, Peri K, Khalifa AAZ, Ghanaeian A, McCafferty CL, Valente-Paterno M, De Bellis C, Huynh PM, Fan Z, Marcotte EM, Wloga D, Bui KH
Nat Commun (2023) 14 pp. 2168-2168 [ DOI: doi:10.1038/s41467-023-37868-0 Pubmed: 37061538 ]
The structure of the Tetrahymena thermophila outer dynein arm on doublet microtubule
Resolution: 8.0 Å
EM Method: Single-particle
Fitted PDBs: 7moq
Q-score: 0.067
Kubo S, Yang SK, Black CS, Dai D, Valente-Paterno M, Gaertig J, Ichikawa M, Bui KH
EMBO Rep (2021) 22 pp. e52911-e52911 [ DOI: doi:10.15252/embr.202152911 Pubmed: 34338432 ]
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila CU428)
- Outer dynein arm docking complex protein oda protein (63 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain tctex-type 1 protein (13 kDa, Protein from Tetrahymena thermophila CU428)
- Docking complex 1/2 protein (11 kDa, Protein from Tetrahymena thermophila CU428)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Tubulin alpha chain (49 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain 2a (15 kDa, Protein from Tetrahymena thermophila CU428)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Thioredoxin (12 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein-1-alpha heavy chain, flagellar inner arm i1 complex protein, putative (475 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (12 kDa, Protein from Tetrahymena thermophila CU428)
- Outer dynein arm complex from tetrahymena thermophila (2 MDa, Cellular component from Tetrahymena thermophila CU428)
- Outer arm dynein beta heavy chain (530 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (12 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein intermediate chain 2 (77 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila CU428)
- Outer dynein arm docking complex protein oda protein (35 kDa, Protein from Tetrahymena thermophila CU428)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dynein light chain roadblock-type 2 protein (18 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (13 kDa, Protein from Tetrahymena thermophila CU428)
- Magnesium ion (24 Da, Ligand)
- Dynein light chain roadblock-type 2 protein (14 kDa, Protein from Tetrahymena thermophila CU428)
- Flagellar outer dynein arm intermediate protein, putative (77 kDa, Protein from Tetrahymena thermophila CU428)
- Tubulin beta chain (49 kDa, Protein from Tetrahymena thermophila CU428)
- Docking complex 1 protein (64 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein heavy chain, outer arm protein (534 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila CU428)
Complex of yeast cytoplasmic dynein MTBD-High and MT without DTT
Nishida N, Komori Y, Takarada O, Watanabe A, Tamura S, Kubo S, Shimada I, Kikkawa M
Nat Commun (2020) 11 [ DOI: 10.1038/s41467-020-14842-8 Pubmed: 32098965 ]
Image sets:
Complex of yeast cytoplasmic dynein MTBD-High and MT with DTT
Nishida N, Komori Y, Takarada O, Watanabe A, Tamura S, Kubo S, Shimada I, Kikkawa M
Nat Commun (2020) 11 [ DOI: 10.1038/s41467-020-14842-8 Pubmed: 32098965 ]
Image sets:
Complex of yeast cytoplasmic dynein MTBD-High and MT without DTT
Resolution: 3.94 Å
EM Method: Helical reconstruction
Fitted PDBs: 6kio
Q-score: 0.414
Nishida N, Komori Y, Takarada O, Watanabe A, Tamura S, Kubo S, Shimada I, Kikkawa M
Nat Commun (2020) 11 pp. 1038-1038 [ Pubmed: 32098965 DOI: doi:10.1038/s41467-020-14842-8 ]
- Tubulin beta chain (47 kDa, Protein from Pig)
- Tubulin alpha-1a chain (45 kDa, Protein from Pig)
- Mtbd-high/mt complex without dtt (Cell from Sus scrofa)
- Dynein heavy chain, cytoplasmic (15 kDa, Protein from Saccharomyces cerevisiae S288c)