S. cerevisiae nexus-sCMGE after DNA replication initiation
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 8p5e
Q-score: 0.39
Henrikus SS, Gross MH, Willhoft O, Puhringer T, Lewis JS, McClure AW, Greiwe JF, Palm G, Nans A, Diffley JFX, Costa A
Nat Struct Mol Biol (2024) [ Pubmed: 38760633 DOI: doi:10.1038/s41594-024-01280-z ]
- Dna replication complex gins protein psf3 (25 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (111 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna (19-mer) (5 kDa, DNA from synthetic construct)
- Dna replication licensing factors mcm2-7 (Complex from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase epsilon subunit b (78 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dna replication complex gins protein psf1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Cdc45 (Complex from Saccharomyces cerevisiae)
- Cell division control protein 45 (75 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase epsilon contains catalytic subunit a and non-catalytic subunit b (Complex from Saccharomyces cerevisiae)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae)
- Dna (Complex from synthetic construct)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
- Dna (7-mer) (2 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Dna polymerase epsilon catalytic subunit a (255 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf2 (25 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein sld5 (33 kDa, Protein from Saccharomyces cerevisiae)
- Complex of mcm2-7, cdc45, gins (psf1-3, sld5) dna polymerase epsilon (subunit a and b), 19-mer dna molecule with complementary 7-mer dna molecule (Complex from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins containing psf1, psf2, psf3 and sld5 (Complex from Saccharomyces cerevisiae)
S. cerevisiae ssDNA-sCMGE after DNA replication initiation
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 8p62
Q-score: 0.382
Henrikus SS, Gross MH, Willhoft O, Puhringer T, Lewis JS, McClure AW, Greiwe JF, Palm G, Nans A, Diffley JFX, Costa A
Nat Struct Mol Biol (2024) [ Pubmed: 38760633 DOI: doi:10.1038/s41594-024-01280-z ]
- Dna replication complex gins protein psf3 (25 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (111 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Complex of mcm2-7, cdc45, gins (psf1-3, sld5) dna polymerase epsilon (subunit a and b), 9-mer dna molecule (Complex)
- Dna replication licensing factors mcm2-7 (Complex from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase epsilon subunit b (78 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dna replication complex gins protein psf1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Cell division control protein 45 (75 kDa, Protein from Saccharomyces cerevisiae)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
- Cell division control protein 45, and dna polymerase epsilon subunit a and b (Complex from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Dna polymerase epsilon catalytic subunit a (255 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf2 (25 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein sld5 (33 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Dna (9-mer) (2 kDa, DNA from synthetic construct)
- Dna replication complex gins containing psf1, psf2, psf3 and sld5 (Complex from Saccharomyces cerevisiae)
- Dna (9-mer) (Complex from synthetic construct)
S. cerevisiae consensus-sCMGE on ssDNA after DNA replication initiation
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8p63
Q-score: 0.4
Henrikus SS, Gross MH, Willhoft O, Puhringer T, Lewis JS, McClure AW, Greiwe JF, Palm G, Nans A, Diffley JFX, Costa A
Nat Struct Mol Biol (2024) [ DOI: doi:10.1038/s41594-024-01280-z Pubmed: 38760633 ]
- Dna polymerase epsilon contains catalytic subunit a and non-catalytic subunit b (Complex from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (111 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae)
- Cell division control protein 45 (75 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Dna replication complex gins protein sld5 (33 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf3 (25 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dna (Complex from synthetic construct)
- Dna (9-mer) (2 kDa, DNA from synthetic construct)
- Dna replication licensing factors mcm2-7 (Complex from Saccharomyces cerevisiae)
- Complex of mcm2-7, cdc45, gins (psf1-3, sld5) dna polymerase epsilon (subunit a and b), 9-mer dna molecule (Complex)
- Magnesium ion (24 Da, Ligand)
- Dna replication complex gins containing psf1, psf2, psf3 and sld5 (Complex from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Cdc45 (Complex from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase epsilon catalytic subunit a (255 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase epsilon subunit b (78 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf2 (25 kDa, Protein from Saccharomyces cerevisiae)
S. cerevisiae sCMGE with N-ter Mcm10 density
Henrikus SS, Gross MH, Willhoft O, Puhringer T, Lewis JS, McClure AW, Greiwe JF, Palm G, Nans A, Diffley JFX, Costa A
Nat Struct Mol Biol (2024) [ Pubmed: 38760633 DOI: doi:10.1038/s41594-024-01280-z ]
- Dna strand 1 (DNA from Saccharomyces cerevisiae)
- Dna replication licensing factors mcm2-7 (Complex from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm4 (Protein from Saccharomyces cerevisiae)
- Cdc45 (Complex from Saccharomyces cerevisiae)
- Dna strand 2 (DNA from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf3 (Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (Protein from Saccharomyces cerevisiae)
- Cell division control protein 45 (Protein from Saccharomyces cerevisiae)
- Dna polymerase epsilon contains catalytic subunit a and non-catalytic subunit b (Complex from Saccharomyces cerevisiae)
- Dna replication complex gins protein sld5 (Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf2 (Protein from Saccharomyces cerevisiae)
- Dna (Complex from DNA molecule)
- Dna replication complex gins protein psf1 (Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (Protein from DNA molecule)
- Dna replication licensing factor mcm5 (Protein from Saccharomyces cerevisiae)
- Complex of mcm2-7, cdc45, gins (psf1-3, sld5) dna polymerase epsilon (subunit a and b), 19-mer dna molecule with complementary 7-mer dna molecule (Complex from Saccharomyces cerevisiae)
- Minichromosome maintenance protein 10 (Protein from Saccharomyces cerevisiae)
- Dna replication complex gins containing psf1, psf2, psf3 and sld5 (Complex from Saccharomyces cerevisiae)
S. cerevisiae CMGE nucleating origin DNA melting
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7qhs
Q-score: 0.473
Lewis JS, Gross MH, Sousa J, Henrikus SS, Greiwe JF, Nans A, Diffley JFX, Costa A
Nature (2022) 606 pp. 1007-1014 [ Pubmed: 35705812 DOI: doi:10.1038/s41586-022-04829-4 ]
- Dna (26-mer) (7 kDa, DNA from DNA molecule)
- Dna (26-mer) (8 kDa, DNA from DNA molecule)
- Dna replication licensing factor mcm3 (111 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Cell division control protein 45 (75 kDa, Protein from Saccharomyces cerevisiae)
- S. cerevisiae cmg helicase nucleating origin dna melting (Complex from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Dna replication complex gins protein sld5 (33 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf3 (25 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dna replication licensing factor mcm5 (86 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase epsilon catalytic subunit a (255 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase epsilon subunit b (78 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf2 (25 kDa, Protein from Saccharomyces cerevisiae)
S. cerevisiae CMGE dimer nucleating origin DNA melting
Lewis JS, Gross MH, Sousa J, Henrikus SS, Greiwe JF, Nans A, Diffley JFX, Costa A
Nature (2022) 606 pp. 1007-1014 [ Pubmed: 35705812 DOI: doi:10.1038/s41586-022-04829-4 ]
S. cerevisiae CMGE dimer nucleating origin DNA melting
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7z13
Q-score: 0.471
Lewis JS, Gross MH, Sousa J, Henrikus SS, Greiwe JF, Nans A, Diffley JFX, Costa A
Nature (2022) 606 pp. 1007-1014 [ Pubmed: 35705812 DOI: doi:10.1038/s41586-022-04829-4 ]
- Dna replication licensing factor mcm3 (111 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna helicase (86 kDa, Protein from Saccharomyces cerevisiae)
- Cell division control protein 45 (75 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Dna replication complex gins protein sld5 (33 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf3 (25 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- S. cerevisiae cmge dimer nucleating origin dna melting (Complex from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase epsilon catalytic subunit a (255 kDa, Protein from Saccharomyces cerevisiae)
- Dna (53-mer) (16 kDa, DNA from DNA molecule)
- Dna polymerase epsilon subunit b (78 kDa, Protein from Saccharomyces cerevisiae)
- Dna (53-mer) (16 kDa, DNA from DNA molecule)
- Dna replication complex gins protein psf2 (25 kDa, Protein from Saccharomyces cerevisiae)