Mycobacterium tuberculosis RNAP paused elongation complex with NusG transcription factor
Resolution: 2.94 Å
EM Method: Single-particle
Fitted PDBs: 8e74
Q-score: 0.543
Delbeau M, Omollo EO, Froom R, Koh S, Mooney RA, Lilic M, Brewer JJ, Rock J, Darst SA, Campbell EA, Landick R
Mol Cell (2023) 83 pp. 1474-1488.e8 [ Pubmed: 37116494 DOI: doi:10.1016/j.molcel.2023.04.007 ]
- Dna (54-mer) (16 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta' (147 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta (129 kDa, Protein from Mycobacterium tuberculosis)
- Zinc ion (65 Da, Ligand)
- Rna (42-mer) (13 kDa, RNA from synthetic construct)
- Dna (54-mer) (16 kDa, DNA from synthetic construct)
- Mycobacterium tuberculosis rnap paused elongation complex with nusg transcription factor (Complex from Mycobacterium tuberculosis)
- Transcription termination/antitermination protein nusg (25 kDa, Protein from Mycobacterium tuberculosis)
Resolution: 3.71 Å
EM Method: Single-particle
Fitted PDBs: 8e79
Q-score: 0.449
Delbeau M, Omollo EO, Froom R, Koh S, Mooney RA, Lilic M, Brewer JJ, Rock J, Darst SA, Campbell EA, Landick R
Mol Cell (2023) 83 pp. 1474-1488.e8 [ Pubmed: 37116494 DOI: doi:10.1016/j.molcel.2023.04.007 ]
- Dna (54-mer) (16 kDa, DNA from synthetic construct)
- Mycobacterium tuberculosis rnap paused elongation complex with escherchia coli nusg transcription factor (Complex from Mycobacterium tuberculosis)
- Magnesium ion (24 Da, Ligand)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta' (147 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta (129 kDa, Protein from Mycobacterium tuberculosis)
- Zinc ion (65 Da, Ligand)
- Dna (54-mer) (16 kDa, DNA from synthetic construct)
- Rna (42-mer) (13 kDa, RNA from synthetic construct)
Mycobacterium tuberculosis RNAP elongation complex with NusG transcription factor
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 8e82
Q-score: 0.447
Delbeau M, Omollo EO, Froom R, Koh S, Mooney RA, Lilic M, Brewer JJ, Rock J, Darst SA, Campbell EA, Landick R
Mol Cell (2023) 83 pp. 1474-1488.e8 [ Pubmed: 37116494 DOI: doi:10.1016/j.molcel.2023.04.007 ]
- Dna (54-mer) (16 kDa, DNA from synthetic construct)
- Rna (5'-r(p*cp*gp*gp*ap*gp*ap*gp*gp*up*a)-3') (6 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase subunit beta (129 kDa, Protein from Mycobacterium tuberculosis)
- Mycobacterium tuberculosis rnap elongation complex with nusg transcription factor (Complex from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis)
- Dna (54-mer) (16 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (147 kDa, Protein from Mycobacterium tuberculosis)
- Transcription termination/antitermination protein nusg (25 kDa, Protein from Mycobacterium tuberculosis)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis)
Mycobacterium tuberculosis RNAP paused elongation complex
Resolution: 3.13 Å
EM Method: Single-particle
Fitted PDBs: 8e8m
Q-score: 0.496
Delbeau M, Omollo EO, Froom R, Koh S, Mooney RA, Lilic M, Brewer JJ, Rock J, Darst SA, Campbell EA, Landick R
Mol Cell (2023) 83 pp. 1474-1488.e8 [ Pubmed: 37116494 DOI: doi:10.1016/j.molcel.2023.04.007 ]
- Dna (54-mer) (16 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis)
- Mycobacterium tuberculosis rnap paused elongation complex (Complex from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta' (147 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta (129 kDa, Protein from Mycobacterium tuberculosis)
- Zinc ion (65 Da, Ligand)
- Dna (54-mer) (16 kDa, DNA from synthetic construct)
- Rna (42-mer) (13 kDa, RNA from synthetic construct)
Mycobacterium tuberculosis RNAP elongation complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8e95
Q-score: 0.497
Delbeau M, Omollo EO, Froom R, Koh S, Mooney RA, Lilic M, Brewer JJ, Rock J, Darst SA, Campbell EA, Landick R
Mol Cell (2023) 83 pp. 1474-1488.e8 [ Pubmed: 37116494 DOI: doi:10.1016/j.molcel.2023.04.007 ]
- Mycobacterium tuberculosis rnap elongation complex (Complex from Mycobacterium tuberculosis)
- Rna (5'-r(p*cp*gp*gp*ap*gp*ap*gp*gp*up*a)-3') (6 kDa, RNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta' (147 kDa, Protein from Mycobacterium tuberculosis)
- Dna (25-mer) (16 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit beta (129 kDa, Protein from Mycobacterium tuberculosis)
- Zinc ion (65 Da, Ligand)
- Dna (33-mer) (16 kDa, DNA from synthetic construct)
Resolution: 3.18 Å
EM Method: Single-particle
Fitted PDBs: 8dy7
Q-score: 0.482
Lilic M, Holmes NA, Bush MJ, Marti AK, Widdick DA, Findlay KC, Choi YJ, Froom R, Koh S, Buttner MJ, Campbell EA
PNAS (2023) 120 pp. e2220785120-e2220785120 [ DOI: doi:10.1073/pnas.2220785120 Pubmed: 36888660 ]
- Iron/sulfur cluster (351 Da, Ligand)
- Transcriptional regulator whib (10 kDa, Protein from Streptomyces venezuelae)
- Dna (78-mer) (30 kDa, DNA from Streptomyces venezuelae)
- Dna (71-mer) (30 kDa, DNA from Streptomyces venezuelae)
- Dna-directed rna polymerase subunit beta' (144 kDa, Protein from Streptomyces venezuelae)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Streptomyces venezuelae)
- Probable cell division protein whia (37 kDa, Protein from Streptomyces venezuelae)
- Rna polymerase sigma factor siga (56 kDa, Protein from Streptomyces venezuelae)
- Rna polymerase-binding protein rbpa (14 kDa, Protein from Streptomyces venezuelae)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta (130 kDa, Protein from Streptomyces venezuelae)
- Dna-directed rna polymerase subunit omega (9 kDa, Protein from Streptomyces venezuelae)
- Streptomyces venezuelae rnap transcription open promoter complex with whia and whib transcription factors (Complex from Streptomyces venezuelae)
Resolution: 3.12 Å
EM Method: Single-particle
Fitted PDBs: 8dy9
Q-score: 0.492
Lilic M, Holmes NA, Bush MJ, Marti AK, Widdick DA, Findlay KC, Choi YJ, Froom R, Koh S, Buttner MJ, Campbell EA
PNAS (2023) 120 pp. e2220785120-e2220785120 [ Pubmed: 36888660 DOI: doi:10.1073/pnas.2220785120 ]
- Iron/sulfur cluster (351 Da, Ligand)
- Dna (38-mer) (15 kDa, DNA from Streptomyces venezuelae)
- Magnesium ion (24 Da, Ligand)
- Rna polymerase sigma factor siga (56 kDa, Protein from Streptomyces venezuelae)
- Rna polymerase-binding protein rbpa (14 kDa, Protein from Streptomyces venezuelae)
- Dna-directed rna polymerase subunit beta' (144 kDa, Protein from Streptomyces venezuelae)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Streptomyces venezuelae ATCC 10712)
- Probable cell division protein whia (34 kDa, Protein from Streptomyces venezuelae)
- Zinc ion (65 Da, Ligand)
- Dna (38-mer) (15 kDa, DNA from Streptomyces venezuelae)
- Dna-directed rna polymerase subunit omega (9 kDa, Protein from Streptomyces venezuelae)
- Transcriptional regulator whib (10 kDa, Protein from Streptomyces venezuelae)
- Dna-directed rna polymerase subunit beta (130 kDa, Protein from Streptomyces venezuelae)
- Streptomyces venezuelae rnap unconstrained open promoter complex with whia and whib transcription factors (Complex from Streptomyces venezuelae)
CryoEM of Mycobacterium tuberculosis WT RNAP holoenzyme/RbpA bound to the Fdx and upstream fork DNA
Boyaci H, Chen J, Lilic M, Palka M, Mooney RA, Landick R, Darst SA, Campbell EA
Elife (2018) 7 [ Pubmed: 29480804 DOI: 10.7554/elife.34823 ]
Image sets:
CryoEM of Mycobacterium tuberculosis WT RNAP holoenzyme/RbpA/Fdx
Boyaci H, Chen J, Lilic M, Palka M, Mooney RA, Landick R, Darst SA, Campbell EA
Elife (2018) 7 pp. 1-19 [ Pubmed: 29480804 DOI: 10.7554/elife.34823 ]
Image sets:
CryoEM of Mycobacterium tuberculosis WT RNAP holoenzyme/RbpA
Boyaci H, Chen J, Lilic M, Palka M, Mooney RA, Landick R, Darst SA, Campbell EA
Elife (2018) 7 pp. 1-19 [ Pubmed: 29480804 DOI: 10.7554/elife.34823 ]
Image sets: