Cry-EM structure of cannabinoid receptor-arrestin 2 complex
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8wrz
Q-score: 0.24
Wang Y, Wu L, Wang T, Liu J, Li F, Jiang L, Fan Z, Yu Y, Chen N, Sun Q, Tan Q, Hua T, Liu ZJ
Protein Cell (2024) 15 pp. 230-234 [ Pubmed: 38102480 DOI: doi:10.1093/procel/pwad055 ]
- Scfv30 light chain, scfv30 heavy chain (28 kDa, Protein from Phage display vector pTDisp)
- Beta-arrestin-1 (44 kDa, Protein from Homo sapiens)
- Cry-em structure of cannabinoid receptor-arrestin 2 complex (Complex from Homo sapiens)
- Soluble cytochrome b562,cannabinoid receptor 1 (53 kDa, Protein from Homo sapiens)
- Vasopressin v2 receptor (2 kDa, Protein from Homo sapiens)
- (6~{a}~{r},9~{r},10~{a}~{r})-9-(hydroxymethyl)-3-(8-isothiocyanato-2-methyl-octan-2-yl)-6,6-dimethyl-6~{a},7,8,9,10,10~{a}-hexahydrobenzo[c]chromen-1-ol (445 Da, Ligand)
Cryo-EM structure of CB2-G protein complex
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 8guq
Q-score: 0.506
Li X, Chang H, Bouma J, de Paus LV, Mukhopadhyay P, Paloczi J, Mustafa M, van der Horst C, Kumar SS, Wu L, Yu Y, van den Berg RJBHN, Janssen APA, Lichtman A, Liu ZJ, Pacher P, van der Stelt M, Heitman LH, Hua T
Nat Commun (2023) 14 pp. 1447-1447 [ Pubmed: 36922494 DOI: doi:10.1038/s41467-023-37112-9 ]
- Ternary complex of cannabinoid receptor 2 with guanine nucleotide-binding protein and single-chain variable fragment (140 MDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Cannabinoid receptor 2 (39 kDa, Protein from Homo sapiens)
- Olorinab (357 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Homo sapiens)
Cryo-EM structure of CP-CB2-G protein complex
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 8gur
Q-score: 0.458
Li X, Chang H, Bouma J, de Paus LV, Mukhopadhyay P, Paloczi J, Mustafa M, van der Horst C, Kumar SS, Wu L, Yu Y, van den Berg RJBHN, Janssen APA, Lichtman A, Liu ZJ, Pacher P, van der Stelt M, Heitman LH, Hua T
Nat Commun (2023) 14 pp. 1447-1447 [ Pubmed: 36922494 DOI: doi:10.1038/s41467-023-37112-9 ]
- Ternary complex of cannabinoid receptor 2 with guanine nucleotide-binding protein and single-chain variable fragment (140 MDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Cannabinoid receptor 2 (39 kDa, Protein from Homo sapiens)
- 2-[(1r,2r,5r)-5-hydroxy-2-(3-hydroxypropyl)cyclohexyl]-5-(2-methyloctan-2-yl)phenol (376 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Homo sapiens)
Cryo-EM structure of HU-CB2-G protein complex
Resolution: 2.97 Å
EM Method: Single-particle
Fitted PDBs: 8gus
Q-score: 0.458
Li X, Chang H, Bouma J, de Paus LV, Mukhopadhyay P, Paloczi J, Mustafa M, van der Horst C, Kumar SS, Wu L, Yu Y, van den Berg RJBHN, Janssen APA, Lichtman A, Liu ZJ, Pacher P, van der Stelt M, Heitman LH, Hua T
Nat Commun (2023) 14 pp. 1447-1447 [ DOI: doi:10.1038/s41467-023-37112-9 Pubmed: 36922494 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- [(1~{s},4~{s},5~{s})-4-[2,6-dimethoxy-4-(2-methyloctan-2-yl)phenyl]-6,6-dimethyl-2-bicyclo[3.1.1]hept-2-enyl]methanol (414 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Ternary complex of cannabinoid receptor 2 with guanine nucleotide-binding protein and single-chain variable fragment (140 MDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Cannabinoid receptor 2 (39 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Homo sapiens)
Cryo-EM structure of LEI-CB2-Gi complex
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 8gut
Q-score: 0.457
Li X, Chang H, Bouma J, de Paus LV, Mukhopadhyay P, Paloczi J, Mustafa M, van der Horst C, Kumar SS, Wu L, Yu Y, van den Berg RJBHN, Janssen APA, Lichtman A, Liu ZJ, Pacher P, van der Stelt M, Heitman LH, Hua T
Nat Commun (2023) 14 pp. 1447-1447 [ Pubmed: 36922494 DOI: doi:10.1038/s41467-023-37112-9 ]
- Ternary complex of cannabinoid receptor 2 with guanine nucleotide-binding protein and single-chain variable fragment (140 MDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Cannabinoid receptor 2 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein subunit gamma (7 kDa, Protein from Homo sapiens)
- 1-[[4-[5-fluoranyl-6-[(oxan-4-ylamino)methyl]pyridin-2-yl]phenyl]methyl]-3-(2-methylpropyl)imidazolidine-2,4-dione (454 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Homo sapiens)
Cryo-EM Structure of Human Niacin Receptor HCA2-Gi protein complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7xk2
Q-score: 0.471
Yang Y, Kang HJ, Gao R, Wang J, Han GW, DiBerto JF, Wu L, Tong J, Qu L, Wu Y, Pileski R, Li X, Zhang XC, Zhao S, Kenakin T, Wang Q, Stevens RC, Peng W, Roth BL, Rao Z, Liu ZJ
Nat Commun (2023) 14 pp. 1692-1692 [ Pubmed: 36973264 DOI: doi:10.1038/s41467-023-37177-6 ]
- 2-[[2,2-dimethyl-3-[3-(5-oxidanylpyridin-2-yl)-1,2,4-oxadiazol-5-yl]propanoyl]amino]cyclohexene-1-carboxylic acid (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Hydroxycarboxylic acid receptor 2 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Niacin receptor hca2-gi complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Homo sapiens)
Cryo-EM structure of a class T GPCR in apo state
Resolution: 3.01 Å
EM Method: Single-particle
Fitted PDBs: 7xp4
Q-score: 0.428
Xu W, Wu L, Liu S, Liu X, Cao X, Zhou C, Zhang J, Fu Y, Guo Y, Wu Y, Tan Q, Wang L, Liu J, Jiang L, Fan Z, Pei Y, Yu J, Cheng J, Zhao S, Hao X, Liu ZJ, Hua T
Science (2022) 377 pp. 1298-1304 [ Pubmed: 36108005 DOI: doi:10.1126/science.abo1633 ]
- Complex of gpcr and g protein (Complex from Homo sapiens)
- Endoglucanase h,taste receptor type 2 member 46,endoglucanase h,taste receptor type 2 member 46,bitter taste receptor t2r46 (91 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(t) subunit alpha-3 (30 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
Cryo-EM structure of a class T GPCR in ligand-free state
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 7xp5
Q-score: 0.412
Xu W, Wu L, Liu S, Liu X, Cao X, Zhou C, Zhang J, Fu Y, Guo Y, Wu Y, Tan Q, Wang L, Liu J, Jiang L, Fan Z, Pei Y, Yu J, Cheng J, Zhao S, Hao X, Liu ZJ, Hua T
Science (2022) 377 pp. 1298-1304 [ Pubmed: 36108005 DOI: doi:10.1126/science.abo1633 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Endoglucanase h,taste receptor type 2 member 46,bitter taste receptor t2r46 (91 kDa, Protein from Homo sapiens)
- Complex of gpcr and g protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(t) subunit alpha-3 (30 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Homo sapiens)
Cryo-EM structure of a class T GPCR in active state
Resolution: 3.01 Å
EM Method: Single-particle
Fitted PDBs: 7xp6
Q-score: 0.415
Xu W, Wu L, Liu S, Liu X, Cao X, Zhou C, Zhang J, Fu Y, Guo Y, Wu Y, Tan Q, Wang L, Liu J, Jiang L, Fan Z, Pei Y, Yu J, Cheng J, Zhao S, Hao X, Liu ZJ, Hua T
Science (2022) 377 pp. 1298-1304 [ DOI: doi:10.1126/science.abo1633 Pubmed: 36108005 ]
- Nanobody 35 (15 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(t) subunit alpha-3 (30 kDa, Protein from Homo sapiens)
- Strychnine (334 Da, Ligand)
- Endoglucanase h,taste receptor type 2 member 46,endoglucanase h,taste receptor type 2 member 46,bitter taste receptor t2r46 (91 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Complex of gpcr and g protein (Complex from Homo sapiens)
Cryo-EM structure of a class A orphan GPCR in complex with Gi
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7vug
Q-score: 0.445
Zhou Y, Daver H, Trapkov B, Wu L, Wu M, Harpsoe K, Gentry PR, Liu K, Larionova M, Liu J, Chen N, Brauner-Osborne H, Gloriam DE, Hua T, Liu ZJ
Cell Res (2022) 32 pp. 210-213 [ DOI: doi:10.1038/s41422-021-00591-w Pubmed: 34916631 ]
- Scfv16 (Complex)
- 3-chloranyl-n-[2-oxidanylidene-2-[[(1s)-1-phenylethyl]amino]ethyl]benzamide (316 Da, Ligand)
- Chimera of endo-1,4-beta-xylanase and probable g-protein coupled receptor 139 (60 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Ternary complex of ligand, gpcr and g protein (180 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Bacillus circulans)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (Complex from Homo sapiens)
- Endo-1,4-beta-xylanase,probable g-protein coupled receptor 139 (Complex from Homo sapiens)
- Scfv16 (27 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)