Cry-EM structure of cannabinoid receptor-arrestin 2 complex
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8wrz
Q-score: 0.24
Wang Y, Wu L, Wang T, Liu J, Li F, Jiang L, Fan Z, Yu Y, Chen N, Sun Q, Tan Q, Hua T, Liu ZJ
Protein Cell (2024) 15 pp. 230-234 [ Pubmed: 38102480 DOI: doi:10.1093/procel/pwad055 ]
- Scfv30 light chain, scfv30 heavy chain (28 kDa, Protein from Phage display vector pTDisp)
- Beta-arrestin-1 (44 kDa, Protein from Homo sapiens)
- Cry-em structure of cannabinoid receptor-arrestin 2 complex (Complex from Homo sapiens)
- Soluble cytochrome b562,cannabinoid receptor 1 (53 kDa, Protein from Homo sapiens)
- Vasopressin v2 receptor (2 kDa, Protein from Homo sapiens)
- (6~{a}~{r},9~{r},10~{a}~{r})-9-(hydroxymethyl)-3-(8-isothiocyanato-2-methyl-octan-2-yl)-6,6-dimethyl-6~{a},7,8,9,10,10~{a}-hexahydrobenzo[c]chromen-1-ol (445 Da, Ligand)
Cryo-EM structure of CB2-G protein complex
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 8guq
Q-score: 0.506
Li X, Chang H, Bouma J, de Paus LV, Mukhopadhyay P, Paloczi J, Mustafa M, van der Horst C, Kumar SS, Wu L, Yu Y, van den Berg RJBHN, Janssen APA, Lichtman A, Liu ZJ, Pacher P, van der Stelt M, Heitman LH, Hua T
Nat Commun (2023) 14 pp. 1447-1447 [ Pubmed: 36922494 DOI: doi:10.1038/s41467-023-37112-9 ]
- Ternary complex of cannabinoid receptor 2 with guanine nucleotide-binding protein and single-chain variable fragment (140 MDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Cannabinoid receptor 2 (39 kDa, Protein from Homo sapiens)
- Olorinab (357 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Homo sapiens)
Cryo-EM structure of CP-CB2-G protein complex
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 8gur
Q-score: 0.458
Li X, Chang H, Bouma J, de Paus LV, Mukhopadhyay P, Paloczi J, Mustafa M, van der Horst C, Kumar SS, Wu L, Yu Y, van den Berg RJBHN, Janssen APA, Lichtman A, Liu ZJ, Pacher P, van der Stelt M, Heitman LH, Hua T
Nat Commun (2023) 14 pp. 1447-1447 [ Pubmed: 36922494 DOI: doi:10.1038/s41467-023-37112-9 ]
- Ternary complex of cannabinoid receptor 2 with guanine nucleotide-binding protein and single-chain variable fragment (140 MDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Cannabinoid receptor 2 (39 kDa, Protein from Homo sapiens)
- 2-[(1r,2r,5r)-5-hydroxy-2-(3-hydroxypropyl)cyclohexyl]-5-(2-methyloctan-2-yl)phenol (376 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Homo sapiens)
Cryo-EM structure of HU-CB2-G protein complex
Resolution: 2.97 Å
EM Method: Single-particle
Fitted PDBs: 8gus
Q-score: 0.458
Li X, Chang H, Bouma J, de Paus LV, Mukhopadhyay P, Paloczi J, Mustafa M, van der Horst C, Kumar SS, Wu L, Yu Y, van den Berg RJBHN, Janssen APA, Lichtman A, Liu ZJ, Pacher P, van der Stelt M, Heitman LH, Hua T
Nat Commun (2023) 14 pp. 1447-1447 [ DOI: doi:10.1038/s41467-023-37112-9 Pubmed: 36922494 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- [(1~{s},4~{s},5~{s})-4-[2,6-dimethoxy-4-(2-methyloctan-2-yl)phenyl]-6,6-dimethyl-2-bicyclo[3.1.1]hept-2-enyl]methanol (414 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Ternary complex of cannabinoid receptor 2 with guanine nucleotide-binding protein and single-chain variable fragment (140 MDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Cannabinoid receptor 2 (39 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Homo sapiens)
Cryo-EM structure of LEI-CB2-Gi complex
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 8gut
Q-score: 0.457
Li X, Chang H, Bouma J, de Paus LV, Mukhopadhyay P, Paloczi J, Mustafa M, van der Horst C, Kumar SS, Wu L, Yu Y, van den Berg RJBHN, Janssen APA, Lichtman A, Liu ZJ, Pacher P, van der Stelt M, Heitman LH, Hua T
Nat Commun (2023) 14 pp. 1447-1447 [ Pubmed: 36922494 DOI: doi:10.1038/s41467-023-37112-9 ]
- Ternary complex of cannabinoid receptor 2 with guanine nucleotide-binding protein and single-chain variable fragment (140 MDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Cannabinoid receptor 2 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein subunit gamma (7 kDa, Protein from Homo sapiens)
- 1-[[4-[5-fluoranyl-6-[(oxan-4-ylamino)methyl]pyridin-2-yl]phenyl]methyl]-3-(2-methylpropyl)imidazolidine-2,4-dione (454 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Homo sapiens)