Cryo-EM structure of zebrafish TRPM5 in the presence of 1 mM EDTA
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7mbp
Q-score: 0.534
Ruan Z, Haley E, Orozco IJ, Sabat M, Myers R, Roth R, Du J, Lu W
Nat Struct Mol Biol (2021) 28 pp. 604-613 [ Pubmed: 34168372 DOI: doi:10.1038/s41594-021-00607-4 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Transient receptor potential melastatin 5 (132 kDa, Protein from Danio rerio)
- Trpm5 channel (Complex from Danio rerio)
- (2r)-2-(hydroxymethyl)-4-{[(25r)-10alpha,14beta,17beta-spirost-5-en-3beta-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-beta-d-glucopyranoside (841 Da, Ligand)
- (25r)-14beta,17beta-spirost-5-en-3beta-ol (414 Da, Ligand)
Cryo-EM structure of zebrafish TRPM5 in the presence of 5 mM calcium
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 7mbq
Q-score: 0.604
Ruan Z, Haley E, Orozco IJ, Sabat M, Myers R, Roth R, Du J, Lu W
Nat Struct Mol Biol (2021) 28 pp. 604-613 [ DOI: doi:10.1038/s41594-021-00607-4 Pubmed: 34168372 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- (25r)-14beta,17beta-spirost-5-en-3beta-ol (414 Da, Ligand)
- Water (18 Da, Ligand)
- Transient receptor potential melastatin 5 (132 kDa, Protein from Danio rerio)
- Trpm5 channel (Complex from Danio rerio)
- Calcium ion (40 Da, Ligand)
- (2r)-2-(hydroxymethyl)-4-{[(25r)-10alpha,14beta,17beta-spirost-5-en-3beta-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-beta-d-glucopyranoside (841 Da, Ligand)
Zebrafish TRPM5 with 1 mM EDTA in nanodisc
Ruan Z, Haley E, Orozco IJ, Sabat M, Myers R, Roth R, Du J, Lu W
Nat Struct Mol Biol (2021) 28 pp. 604-613 [ Pubmed: 34168372 DOI: doi:10.1038/s41594-021-00607-4 ]
- Zebrafish trpm5 (Protein from Danio rerio)
- Trpm5 channel (Complex from Danio rerio)
Zebrafish TRPM5 with 1 mM calcium and 0.5 mM steviol in nanodisc at 3.0 Angstroms resolution
Ruan Z, Haley E, Orozco IJ, Sabat M, Myers R, Roth R, Du J, Lu W
Nat Struct Mol Biol (2021) 28 pp. 604-613 [ DOI: doi:10.1038/s41594-021-00607-4 Pubmed: 34168372 ]
- Trpm5 channel (Complex from Danio rerio)
- Zebrafish trpm5 (Protein from Danio rerio)
Cryo-EM structure of zebrafish TRPM5 in the presence of 6 uM calcium (apo state)
Ruan Z, Haley E, Orozco IJ, Sabat M, Myers R, Roth R, Du J, Lu W
Nat Struct Mol Biol (2021) 28 pp. 604-613 [ Pubmed: 34168372 DOI: doi:10.1038/s41594-021-00607-4 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Transient receptor potential melastatin 5 (132 kDa, Protein from Danio rerio)
- Trpm5 channel (Complex from Danio rerio)
- (2r)-2-(hydroxymethyl)-4-{[(25r)-10alpha,14beta,17beta-spirost-5-en-3beta-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-beta-d-glucopyranoside (841 Da, Ligand)
- (25r)-14beta,17beta-spirost-5-en-3beta-ol (414 Da, Ligand)
Cryo-EM structure of zebrafish TRPM5 in the presence of 6 uM calcium (open state)
Ruan Z, Haley E, Orozco IJ, Sabat M, Myers R, Roth R, Du J, Lu W
Nat Struct Mol Biol (2021) 28 pp. 604-613 [ DOI: doi:10.1038/s41594-021-00607-4 Pubmed: 34168372 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- (25r)-14beta,17beta-spirost-5-en-3beta-ol (414 Da, Ligand)
- Water (18 Da, Ligand)
- Transient receptor potential melastatin 5 (132 kDa, Protein from Danio rerio)
- Trpm5 channel (Complex from Danio rerio)
- Calcium ion (40 Da, Ligand)
- (2r)-2-(hydroxymethyl)-4-{[(25r)-10alpha,14beta,17beta-spirost-5-en-3beta-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-beta-d-glucopyranoside (841 Da, Ligand)
Ruan Z, Haley E, Orozco IJ, Sabat M, Myers R, Roth R, Du J, Lu W
Nat Struct Mol Biol (2021) 28 pp. 604-613 [ Pubmed: 34168372 DOI: doi:10.1038/s41594-021-00607-4 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Transient receptor potential melastatin 5 (132 kDa, Protein from Danio rerio)
- Trpm5 channel e337a mutant (Complex from Danio rerio)
- (2r)-2-(hydroxymethyl)-4-{[(25r)-10alpha,14beta,17beta-spirost-5-en-3beta-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-beta-d-glucopyranoside (841 Da, Ligand)
- (25r)-14beta,17beta-spirost-5-en-3beta-ol (414 Da, Ligand)
Ruan Z, Haley E, Orozco IJ, Sabat M, Myers R, Roth R, Du J, Lu W
Nat Struct Mol Biol (2021) 28 pp. 604-613 [ Pubmed: 34168372 DOI: doi:10.1038/s41594-021-00607-4 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Transient receptor potential melastatin 5 (132 kDa, Protein from Danio rerio)
- Trpm5 channel e337a mutant (Complex from Danio rerio)
- (2r)-2-(hydroxymethyl)-4-{[(25r)-10alpha,14beta,17beta-spirost-5-en-3beta-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-beta-d-glucopyranoside (841 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- (25r)-14beta,17beta-spirost-5-en-3beta-ol (414 Da, Ligand)
Cryo-EM structure of zebrafish TRPM5 in the presence of 5 mM calcium and 0.5 mM NDNA
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7mbv
Q-score: 0.546
Ruan Z, Haley E, Orozco IJ, Sabat M, Myers R, Roth R, Du J, Lu W
Nat Struct Mol Biol (2021) 28 pp. 604-613 [ DOI: doi:10.1038/s41594-021-00607-4 Pubmed: 34168372 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- N'-[(e)-(3,4-dimethoxyphenyl)methylidene]-2-(naphthalen-1-yl)acetohydrazide (348 Da, Ligand)
- (25r)-14beta,17beta-spirost-5-en-3beta-ol (414 Da, Ligand)
- Transient receptor potential melastatin 5 (132 kDa, Protein from Danio rerio)
- Trpm5 channel (Complex from Danio rerio)
- Calcium ion (40 Da, Ligand)
- (2r)-2-(hydroxymethyl)-4-{[(25r)-10alpha,14beta,17beta-spirost-5-en-3beta-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-beta-d-glucopyranoside (841 Da, Ligand)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6plp
Q-score: 0.422
Yu J, Zhu H, Lape R, Greiner T, Du J, Lu W, Sivilotti L, Gouaux E
Cell (2021) 184 pp. 957-968.e21 [ DOI: doi:10.1016/j.cell.2021.01.026 Pubmed: 33567265 ]
- Glycine receptor subunit alphaz1 (52 kDa, Protein from Danio rerio)
- Gamma-amino-butanoic acid (103 Da, Ligand)
- Glycine receptor in complex with gaba (250 kDa, Complex from Danio rerio)