Cryo-electron tomogram of GEM2-labelled Mito-EGFP in HeLa cells
Fung HKH, Hayashi Y, Salo VT, Babenko A, Zagoriy I, Brunner A, Ellenberg J, Muller CW, Cuylen-Haering S, Mahamid J
Nat Methods (2023) 20 pp. 1900-1908 [ Pubmed: 37932397 DOI: doi:10.1038/s41592-023-02053-0 ]
In situ subtomogram average of GEM2 particles in human cells
Fung HKH, Hayashi Y, Salo VT, Babenko A, Zagoriy I, Brunner A, Ellenberg J, Muller CW, Cuylen-Haering S, Mahamid J
Nat Methods (2023) 20 pp. 1900-1908 [ DOI: doi:10.1038/s41592-023-02053-0 Pubmed: 37932397 ]
- Cryo-focused ion beam lamellae of human cells (hela, u2os, sum159) expressing the engineered genetically encoded multimeric tag gem2 (Cell from Synechococcus elongatus PCC 7942 = FACHB-805)
- Cryo-focused ion beam lamellae of human cells (hela, u2os, sum159) expressing the engineered genetically encoded multimeric tag gem2 (Protein from Homo sapiens)
Local refinement map of TFIIIC TauB-DNA monomer
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8cli
Q-score: 0.492
Seifert-Davila W, Girbig M, Hauptmann L, Hoffmann T, Eustermann S, Muller CW
Sci Adv (2023) 9 pp. eadh2019-eadh2019 [ DOI: doi:10.1126/sciadv.adh2019 Pubmed: 37418517 ]
- Tfiiic taub-dna monomer (300 kDa, Complex from Homo sapiens)
- Dna (35-mer) (10 kDa, DNA from Homo sapiens)
- Dna (35-mer) (10 kDa, DNA from Homo sapiens)
- General transcription factor 3c polypeptide 2 (102 kDa, Protein from Homo sapiens)
- General transcription factor 3c polypeptide 4 (92 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- General transcription factor 3c polypeptide 1 (244 kDa, Protein from Homo sapiens)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8clj
Q-score: 0.49
Seifert-Davila W, Girbig M, Hauptmann L, Hoffmann T, Eustermann S, Muller CW
Sci Adv (2023) 9 pp. eadh2019-eadh2019 [ DOI: doi:10.1126/sciadv.adh2019 Pubmed: 37418517 ]
- Tfiiic taub-dna dimer (600 kDa, Complex from Homo sapiens)
- Tdna (35-mer) (10 kDa, DNA from Homo sapiens)
- Tdna (35-mer) (10 kDa, DNA from Homo sapiens)
- General transcription factor 3c polypeptide 2 (102 kDa, Protein from Homo sapiens)
- General transcription factor 3c polypeptide 4 (92 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- General transcription factor 3c polypeptide 1 (244 kDa, Protein from Homo sapiens)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8clk
Q-score: 0.358
Seifert-Davila W, Girbig M, Hauptmann L, Hoffmann T, Eustermann S, Muller CW
Sci Adv (2023) 9 pp. eadh2019-eadh2019 [ DOI: doi:10.1126/sciadv.adh2019 Pubmed: 37418517 ]
- General transcription factor 3c polypeptide 3 (101 kDa, Protein from Homo sapiens)
- General transcription factor 3c polypeptide 5 (61 kDa, Protein from Homo sapiens)
- General transcription factor 3c polypeptide 6 (24 kDa, Protein from Homo sapiens)
- Tfiiic taua subcomplex (330 kDa, Complex from Homo sapiens)
- General transcription factor 3c polypeptide 1 (244 kDa, Protein from Homo sapiens)
TFIIIC TauA complex map (sample without DNA)
Seifert-Davila W, Girbig M, Hauptmann L, Hoffmann T, Eustermann S, Muller CW
Sci Adv (2023) 9 pp. eadh2019-eadh2019 [ Pubmed: 37418517 DOI: doi:10.1126/sciadv.adh2019 ]
- Tfiiic taua subcomplex (330 kDa, Complex from Homo sapiens)
Structural insights into human TFIIIC promoter recognition
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8cll
Q-score: 0.445
Seifert-Davila W, Girbig M, Hauptmann L, Hoffmann T, Eustermann S, Muller CW
Sci Adv (2023) 9 pp. eadh2019-eadh2019 [ Pubmed: 37418517 DOI: doi:10.1126/sciadv.adh2019 ]
- General transcription factor 3c polypeptide 2 (102 kDa, Protein from Homo sapiens)
- General transcription factor 3c polypeptide 4 (92 kDa, Protein from Homo sapiens)
- General transcription factor 3c polypeptide 1 (244 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Tfiiic taub dimer (560 kDa, Complex from Homo sapiens)
Consensus map of TauB-DNA dimer (Nu-refinement)
Seifert-Davila W, Girbig M, Hauptmann L, Hoffmann T, Eustermann S, Muller CW
Sci Adv (2023) 9 pp. eadh2019-eadh2019 [ Pubmed: 37418517 DOI: doi:10.1126/sciadv.adh2019 ]
- Tfiiic taub-dna dimer (600 kDa, Complex from Homo sapiens)
Local refinement map of TFIIIC TauB-DNA monomer 2
Seifert-Davila W, Girbig M, Hauptmann L, Hoffmann T, Eustermann S, Muller CW
Sci Adv (2023) 9 pp. eadh2019-eadh2019 [ Pubmed: 37418517 DOI: doi:10.1126/sciadv.adh2019 ]
- Tfiiic taub-dna monomer 2 (600 kDa, Complex from Homo sapiens)
Resolution: 4.5 Å
EM Method: Subtomogram averaging
Fitted PDBs: 7ozr
Q-score: 0.356
Zhang X, Sridharan S, Zagoriy I, Eugster Oegema C, Ching C, Pflaesterer T, Fung HKH, Becher I, Poser I, Muller CW, Hyman AA, Savitski MM, Mahamid J
Cell (2023) 186 pp. 1877-1894.e27 [ DOI: doi:10.1016/j.cell.2023.03.015 Pubmed: 37116470 ]
- Nucleocapsid (61 kDa, Protein from Mumps virus genotype A)
- Rna (5'-r(p*up*up*up*up*up*u)-3') (1 kDa, RNA from Mumps virus genotype A)
- Authentic mumps virus nucleocapsid-rna complex (Complex from Mumps virus genotype A)