Structure of 5D3-Fab and nanobody(Nb8)-bound ABCG2
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 8p7w
Q-score: 0.511
Irobalieva RN, Manolaridis I, Jackson SM, Ni D, Pardon E, Stahlberg H, Steyaert J, Locher KP
J Mol Biol (2023) 435 pp. 168234-168234 [ Pubmed: 37597690 DOI: doi:10.1016/j.jmb.2023.168234 ]
- Nanobody (13 kDa, Protein from Lama glama)
- Atp-binding cassette sub-family g member 2 (72 kDa, Protein from Homo sapiens)
- 5d3(fab) light chain variable domain (23 kDa, Protein from Mus musculus)
- Atp-binding cassette sub-family g member 2 (Complex from Homo sapiens)
- Structure of nanobody (nb8)-bound abcg2 (Complex)
- Nanobody (Complex from Lama glama)
- 5d3(fab) heavy and light chain variable domain (Complex from Mus musculus)
- 5d3(fab) heavy chain variable domain (23 kDa, Protein from Mus musculus)
Structure of 5D3-Fab and nanobody(Nb17)-bound ABCG2
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8p8a
Q-score: 0.52
Irobalieva RN, Manolaridis I, Jackson SM, Ni D, Pardon E, Stahlberg H, Steyaert J, Locher KP
J Mol Biol (2023) 435 pp. 168234-168234 [ DOI: doi:10.1016/j.jmb.2023.168234 Pubmed: 37597690 ]
- 5d3(fab) light chain variable domain (23 kDa, Protein from Mus musculus)
- Structure of nanobody (nb8)-bound abcg2 (Complex from Homo sapiens)
- Atp-binding cassette sub-family g member 2 (72 kDa, Protein from Homo sapiens)
- 5d3(fab) heavy chain variable domain (17 kDa, Protein from Mus musculus)
- Nanobody (13 kDa, Protein from Lama glama)
Structure of 5D3-Fab and nanobody(Nb96)-bound ABCG2
Resolution: 3.49 Å
EM Method: Single-particle
Fitted PDBs: 8p8j
Q-score: 0.471
Irobalieva RN, Manolaridis I, Jackson SM, Ni D, Pardon E, Stahlberg H, Steyaert J, Locher KP
J Mol Biol (2023) 435 pp. 168234-168234 [ DOI: doi:10.1016/j.jmb.2023.168234 Pubmed: 37597690 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 5d3(fab) light chain variable domain (23 kDa, Protein from Mus musculus)
- Nanobody (13 kDa, Protein from Lama glama)
- Nanobody (Complex from Lama glama)
- Atp-binding cassette sub-family g member 2 (Complex from Homo sapiens)
- Atp-binding cassette sub-family g member 2 (72 kDa, Protein from Homo sapiens)
- 5d3(fab) heavy chain variable domain (23 kDa, Protein from Mus musculus)
- Structure of nanobody (nb96)-bound abcg2 (Complex)
- 5d3(fab) heavy and light chain variable domain (Complex from Mus musculus)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7oj8
Q-score: 0.437
Yu Q, Ni D, Kowal J, Manolaridis I, Jackson SM, Stahlberg H, Locher KP
Nat Commun (2021) 12 pp. 4376-4376 [ DOI: doi:10.1038/s41467-021-24651-2 Pubmed: 34282134 ]
- Estrone 3-sulfate (350 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Broad substrate specificity atp-binding cassette transporter abcg2 (73 kDa, Protein from Homo sapiens)
- Abcg2 in complex with e1s under turnover condition (144 kDa, Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
ABCG2 topotecan turnover-1 state
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7ojh
Q-score: 0.513
Yu Q, Ni D, Kowal J, Manolaridis I, Jackson SM, Stahlberg H, Locher KP
Nat Commun (2021) 12 pp. 4376-4376 [ DOI: doi:10.1038/s41467-021-24651-2 Pubmed: 34282134 ]
- (s)-10-[(dimethylamino)methyl]-4-ethyl-4,9-dihydroxy-1h-pyrano[3',4':6,7]inolizino[1,2-b]-quinoline-3,14(4h,12h)-dione (421 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Abcg2 in complex with topotecan under turnover condition (144 kDa, Complex from Homo sapiens)
- Broad substrate specificity atp-binding cassette transporter abcg2 (73 kDa, Protein from Homo sapiens)
ABCG2 topotecan turnover-2 state
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7oji
Q-score: 0.485
Yu Q, Ni D, Kowal J, Manolaridis I, Jackson SM, Stahlberg H, Locher KP
Nat Commun (2021) 12 pp. 4376-4376 [ DOI: doi:10.1038/s41467-021-24651-2 Pubmed: 34282134 ]
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Diundecyl phosphatidyl choline (622 Da, Ligand)
- Abcg2 in complex with topotecan under turnover condition (144 kDa, Complex from Homo sapiens)
- Broad substrate specificity atp-binding cassette transporter abcg2 (73 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- (s)-10-[(dimethylamino)methyl]-4-ethyl-4,9-dihydroxy-1h-pyrano[3',4':6,7]inolizino[1,2-b]-quinoline-3,14(4h,12h)-dione (421 Da, Ligand)
Structure of tariquidar-bound ABCG2
Resolution: 3.12 Å
EM Method: Single-particle
Fitted PDBs: 7neq
Q-score: 0.535
Kowal J, Ni D, Jackson SM, Manolaridis I, Stahlberg H, Locher KP
J Mol Biol (2021) 433 pp. 166980-166980 [ Pubmed: 33838147 DOI: doi:10.1016/j.jmb.2021.166980 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Multidrug transporter abcg2 with bound substrate tariquidar and 5d3-fab variable domain (250 kDa, Complex)
- 5d3(fab) light- and heavy-chain variable domains (Complex from Mus musculus)
- 5d3(fab) light chain variable domain (23 kDa, Protein from Mus musculus)
- Atp-binding cassette sub-family g member 2 (Complex from Homo sapiens)
- Atp-binding cassette sub-family g member 2 (72 kDa, Protein from Homo sapiens)
- 5d3(fab) heavy chain variable domain (23 kDa, Protein from Mus musculus)
- [(2~{s})-3-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-2-decanoyloxy-propyl] octadecanoate (635 Da, Ligand)
- Water (18 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Tariquidar (646 Da, Ligand)
Structure of topotecan-bound ABCG2
Resolution: 3.39 Å
EM Method: Single-particle
Fitted PDBs: 7nez
Q-score: 0.362
Kowal J, Ni D, Jackson SM, Manolaridis I, Stahlberg H, Locher KP
J Mol Biol (2021) 433 pp. 166980-166980 [ DOI: doi:10.1016/j.jmb.2021.166980 Pubmed: 33838147 ]
- 5d3(fab) light chain variable domain (23 kDa, Protein from Mus musculus)
- Multidrug transporter abcg2 with bound substrate topotecan and 5d3-fab variable domain (250 kDa, Complex)
- 5d3-fab variable domain (Complex from Mus musculus)
- Atp-binding cassette sub-family g member 2 (72 kDa, Protein from Homo sapiens)
- (s)-10-[(dimethylamino)methyl]-4-ethyl-4,9-dihydroxy-1h-pyrano[3',4':6,7]inolizino[1,2-b]-quinoline-3,14(4h,12h)-dione (421 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 5d3(fab) heavy chain variable domain (23 kDa, Protein from Mus musculus)
- Multidrug transporter abcg2 with bound substrate topotecan (Complex from Homo sapiens)
Structure of mitoxantrone-bound ABCG2
Resolution: 3.51 Å
EM Method: Single-particle
Fitted PDBs: 7nfd
Q-score: 0.462
Kowal J, Ni D, Jackson SM, Manolaridis I, Stahlberg H, Locher KP
J Mol Biol (2021) 433 pp. 166980-166980 [ Pubmed: 33838147 DOI: doi:10.1016/j.jmb.2021.166980 ]
- 5d3(fab) light chain variable domain (23 kDa, Protein from Mus musculus)
- Multidrug transporter abcg2 with bound substrate mitoxantrone (Complex from Homo sapiens)
- Atp-binding cassette sub-family g member 2 (72 kDa, Protein from Homo sapiens)
- 5d3-fab variable domain (Complex from Mus musculus)
- 5d3(fab) heavy chain variable domain (23 kDa, Protein from Mus musculus)
- Multidrug transporter abcg2 with bound substrate mitoxantrone and 5d3-fab variable domain (250 kDa, Complex)
- 1,4-dihydroxy-5,8-bis({2-[(2-hydroxyethyl)amino]ethyl}amino)-9,10-anthracenedione (444 Da, Ligand)
CryoEM reconstruction of human ABCG2 transporter with inhibitor MZ29 and 5D3-Fab
Jackson SM, Manolaridis I, Kowal J, Zechner M, Taylor NMI, Bause M, Bauer S, Bartholomaeus R, Bernhardt G, Koenig B, Buschauer A, Stahlberg H, Altmann KH, Locher KP
Nat. Struct. Mol. Biol. (2018) 25 pp. 333-340 [ DOI: 10.1038/s41594-018-0049-1 Pubmed: 29610494 ]
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