RNA-triggered protein cleavage and cell growth arrest by the type III-E CRISPR nuclease-protease
Kato K, Okazaki S, Schmitt-Ulms C, Jiang K, Zhou W, Ishikawa J, Isayama Y, Adachi S, Nishizawa T, Makarova KS, Koonin EV, Abudayyeh OO, Gootenberg JS, Nishimasu H
Science (2022) 378 pp. 882-889 [ DOI: 10.1126/science.add7347 Pubmed: 36423304 ]
Image sets:
Structure and engineering of the type III-E CRISPR-Cas7-11 effector complex
Kato K, Zhou W, Okazaki S, Isayama Y, Nishizawa T, Gootenberg JS, Abudayyeh OO, Nishimasu H
Cell (2022) 185 pp. 2324-2337.e16 [ Pubmed: 35643083 DOI: 10.1016/j.cell.2022.05.003 ]
Image sets:
Desulfonema ishimotonii Cas7-11 in complex with Csx29 and Csx30
Strecker J, Demircioglu FE, Li D, Faure G, Wilkinson ME, Gootenberg JS, Abudayyeh OO, Nishimasu H, Macrae RK, Zhang F
Science (2022) 378 pp. 874-881 [ DOI: 10.1126/science.add7450 Pubmed: 36423276 ]
Image sets:
Structure of the OgeuIscB-omega RNA-target DNA complex
Resolution: 2.55 Å
EM Method: Single-particle
Fitted PDBs: 7xht
Q-score: 0.619
Kato K, Okazaki S, Kannan S, Altae-Tran H, Esra Demircioglu F, Isayama Y, Ishikawa J, Fukuda M, Macrae RK, Nishizawa T, Makarova KS, Koonin EV, Zhang F, Nishimasu H
Nat Commun (2022) 13 pp. 6719-6719 [ Pubmed: 36344504 DOI: doi:10.1038/s41467-022-34378-3 ]
- Rna (228-mer) (73 kDa, RNA from metagenome)
- Structure of the iscb-omegarna ribonucleoprotein complex (Complex)
- Lauryl dimethylamine-n-oxide (229 Da, Ligand)
- Dna (5'-d(p*gp*ap*ap*gp*ap*ap*ap*ap*cp*cp*ap*t)-3') (4 kDa, DNA from synthetic construct)
- Ogeuiscb (56 kDa, Protein from metagenome)
- Dna (49-mer) (15 kDa, DNA from synthetic construct)
- Iscb-omegarna (Complex from metagenome)
- Magnesium ion (24 Da, Ligand)
- Dna (Complex)
Cas7-11 in complex with Csx29, focus refined on Cas7-11
Resolution: 2.95 Å
EM Method: Single-particle
Fitted PDBs: 8eex
Q-score: 0.537
Strecker J, Demircioglu FE, Li D, Faure G, Wilkinson ME, Gootenberg JS, Abudayyeh OO, Nishimasu H, Macrae RK, Zhang F
Science (2022) 378 pp. 874-881 [ DOI: doi:10.1126/science.add7450 Pubmed: 36423276 ]
- Crrna (11 kDa, RNA from Desulfonema ishimotonii)
- Cas7-11 (183 kDa, Protein from Desulfonema ishimotonii)
- Cas7-11 in complex with csx29 (Complex from Desulfonema ishimotonii)
- Zinc ion (65 Da, Ligand)
- Csx29 (88 kDa, Protein from Desulfonema ishimotonii)
Cas7-11 in complex with DR-mismatched target RNA, Csx29 and Csx30
Resolution: 2.53 Å
EM Method: Single-particle
Fitted PDBs: 8eey
Q-score: 0.586
Strecker J, Demircioglu FE, Li D, Faure G, Wilkinson ME, Gootenberg JS, Abudayyeh OO, Nishimasu H, Macrae RK, Zhang F
Science (2022) 378 pp. 874-881 [ DOI: doi:10.1126/science.add7450 Pubmed: 36423276 ]
- Cas7-11 (183 kDa, Protein from Desulfonema ishimotonii)
- Dr-mismatched target rna (8 kDa, RNA from synthetic construct)
- Csx30 (62 kDa, Protein from Desulfonema ishimotonii)
- Cas7-11 in complex with dr-mismatched target rna, csx29 and csx30 (Complex from Desulfonema ishimotonii)
- Zinc ion (65 Da, Ligand)
- Crrna (12 kDa, RNA from Desulfonema ishimotonii)
- Csx29 (88 kDa, Protein from Desulfonema ishimotonii)
Cas7-11 in complex with Csx29, focus refined on Csx29
Strecker J, Demircioglu FE, Li D, Faure G, Wilkinson ME, Gootenberg JS, Abudayyeh OO, Nishimasu H, Macrae RK, Zhang F
Science (2022) 378 pp. 874-881 [ Pubmed: 36423276 DOI: doi:10.1126/science.add7450 ]
Strecker J, Demircioglu FE, Li D, Faure G, Wilkinson ME, Gootenberg JS, Abudayyeh OO, Nishimasu H, Macrae RK, Zhang F
Science (2022) 378 pp. 874-881 [ Pubmed: 36423276 DOI: doi:10.1126/science.add7450 ]
Cas7-11 in complex with DR-mismatched target RNA, Csx29 and Csx30, focus refined on Csx29
Strecker J, Demircioglu FE, Li D, Faure G, Wilkinson ME, Gootenberg JS, Abudayyeh OO, Nishimasu H, Macrae RK, Zhang F
Science (2022) 378 pp. 874-881 [ Pubmed: 36423276 DOI: doi:10.1126/science.add7450 ]
Strecker J, Demircioglu FE, Li D, Faure G, Wilkinson ME, Gootenberg JS, Abudayyeh OO, Nishimasu H, Macrae RK, Zhang F
Science (2022) 378 pp. 874-881 [ Pubmed: 36423276 DOI: doi:10.1126/science.add7450 ]