Post-catalytic P complex spliceosome with 3' splice site docked
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6exn
Q-score: 0.296
Wilkinson ME, Fica SM, Galej WP, Norman CM, Newman AJ, Nagai K
Science (2017) 358 pp. 1283-1288 [ DOI: doi:10.1126/science.aar3729 Pubmed: 29146871 ]
- Pre-mrna-splicing factor atp-dependent rna helicase prp22 (130 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor slt11 (40 kDa, Protein from Baker's yeast)
- U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor snt309 (20 kDa, Protein from Baker's yeast)
- Pre-mrna-processing protein 45 (42 kDa, Protein from Baker's yeast)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Intron lariat: ubc4 rna (30 kDa, RNA from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor syf2 (24 kDa, Protein from Baker's yeast)
- U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae S288c)
- Magnesium ion (24 Da, Ligand)
- Pre-mrna-splicing factor snu114 (114 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc22 (67 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc16 (32 kDa, Protein from Baker's yeast)
- Ligated exons: ubc4 mrna (12 kDa, RNA from Saccharomyces cerevisiae S288c)
- 3' exon (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-splicing factor prp8 (279 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor slu7 (44 kDa, Protein from Baker's yeast)
- Inositol hexakisphosphate (660 Da, Ligand)
- Pre-mrna-splicing factor prp18 (28 kDa, Protein from Baker's yeast)
- U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor clf1 (82 kDa, Protein from Baker's yeast)
- P complex spliceosome (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-splicing factor cef1 (67 kDa, Protein from Baker's yeast)
- Pre-mrna-processing factor prp19 (56 kDa, Protein from Baker's yeast)
- P complex spliceosome with 3' exon docked (2 MDa, Complex)
- Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor prp46 (50 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
- Zinc ion (65 Da, Ligand)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Baker's yeast)
- Unassigned structure (8 kDa, Protein from Baker's yeast)
- U6 snrna (35 kDa, RNA from Baker's yeast)
- Pre-mrna-splicing factor bud31 (18 kDa, Protein from Baker's yeast)
- U5 snrna (57 kDa, RNA from Baker's yeast)
- Pre-mrna-processing factor prp17 (52 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Baker's yeast)
Post-catalytic P complex spliceosome with 3' splice site undocked
Wilkinson ME, Fica SM, Galej WP, Norman CM, Newman AJ, Nagai K
Science (2017) 358 pp. 1283-1288 [ DOI: doi:10.1126/science.aar3729 Pubmed: 29146871 ]