Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6bbm
Q-score: 0.381
Chase J, Catalano A, Noble AJ, Eng ET, Olinares PD, Molloy K, Pakotiprapha D, Samuels M, Chait B, des Georges A, Jeruzalmi D
eLife (2018) 7 [ Pubmed: 30582519 DOI: doi:10.7554/eLife.41140 ]
- Replicative dna helicase (52 kDa, Protein from Escherichia coli O111:NM)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Lambda p helicase loader (Complex from Enterobacteria phage lambda)
- Dnab helicase - lambda p helicase loader dna replication complex (Complex from Escherichia coli)
- Replication protein p (23 kDa, Protein from Escherichia phage lambda)
- E coli dnab helicase (Complex from Escherichia coli)
CryoEM structure of crosslinked E.coli RNA polymerase elongation complex
Kang JY, Olinares PD, Chen J, Campbell EA, Mustaev A, Chait BT, Gottesman ME, Darst SA
eLife (2017) 6 [ DOI: doi:10.7554/eLife.25478 Pubmed: 28318486 ]
- Dna (29-mer) (8 kDa, DNA from T7likevirus)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli O157:H7)
- Crosslinked e.coli rna polymerase ternary elongation complex (393 kDa, Complex from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli O157:H7)
- Dna (29-mer) (8 kDa, DNA from T7likevirus)
- Rna (5'-r(*gp*cp*a*up*up*cp*ap*ap*ap*gp*cp*gp*gp*ap*gp*ap*gp*gp*up*a)-3') (6 kDa, RNA from T7likevirus)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli O45:K1 (strain S88 / ExPEC))
- Dna-directed rna polymerase subunit omega (9 kDa, Protein from Escherichia coli O45:K1 (strain S88 / ExPEC))
CryoEM structure of HK022 Nun - E.coli RNA polymerase elongation complex
Kang JY, Olinares PD, Chen J, Campbell EA, Mustaev A, Chait BT, Gottesman ME, Darst SA
eLife (2017) 6 [ DOI: doi:10.7554/eLife.25478 Pubmed: 28318486 ]
- Dna (29-mer) (8 kDa, DNA from T7likevirus)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli (strain K12))
- E.coli rna polymerase ternary elongation complex in complex with hk022 nun (405 kDa, Complex from Escherichia Coli)
- Rna (5'-r(*gp*cp*ap*up*up*cp*ap*ap*ap*gp*cp*gp*gp*ap*gp*ap*gp*gp*up*a)-3') (6 kDa, RNA from T7likevirus)
- Transcription termination factor nun (12 kDa, Protein from Escherichia coli (strain K12))
- Dna (29-mer) (8 kDa, DNA from T7likevirus)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli (strain K12))
- Zinc ion (65 Da, Ligand)
CryoEM structure of E.coli RNA polymerase elongation complex
Kang JY, Olinares PD, Chen J, Campbell EA, Mustaev A, Chait BT, Gottesman ME, Darst SA
eLife (2017) 6 [ DOI: doi:10.7554/eLife.25478 Pubmed: 28318486 ]
- Dna (29-mer) (8 kDa, DNA from T7likevirus)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli (strain K12))
- Zinc ion (65 Da, Ligand)
- Dna (29-mer) (8 kDa, DNA from T7likevirus)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit omega (9 kDa, Protein from Escherichia coli (strain K12))
- Rna (5'-r(*gp*cp*a*up*up*cp*ap*ap*ap*gp*cp*gp*gp*ap*gp*ap*gp*gp*up*a)-3') (6 kDa, RNA from T7likevirus)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli (strain K12))
- E.coli rna polymerase ternary elongation complex (393 kDa, Complex from Escherichia coli)