Ex vivo L-ENA type pili from Bacillus paranthracis endospores
To Be Published
- Ena3a (Protein from Bacillus paranthracis)
- L-type endospore appendage (18 MDa, Cellular component from Bacillus paranthracis)
SARS-CoV-2 S protein bound to human neutralising antibody UZGENT_G5
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8qpr
Q-score: 0.469
Acar DD, Witkowski W, Wejda M, Wei R, Desmet T, Schepens B, De Cae S, Sedeyn K, Eeckhaut H, Fijalkowska D, Roose K, Vanmarcke S, Poupon A, Jochmans D, Zhang X, Abdelnabi R, Foo CS, Weynand B, Reiter D, Callewaert N, Remaut H, Neyts J, Saelens X, Gerlo S, Vandekerckhove L
Ebiomedicine (2024) 100 pp. 104960-104960 [ DOI: doi:10.1016/j.ebiom.2023.104960 Pubmed: 38232633 ]
- Fab fragment of neutralising human antibody uzgent_g5 (Complex from Homo sapiens)
- Sars-cov2 s protein with fibritin (trimerization) tag (Complex from Severe acute respiratory syndrome coronavirus 2)
- Complex of sars-cov2 s protein (wuhan) and fab fragment of neutralising human antibody uzgent_g5 (Complex)
- Igg heavy chain - fab (24 kDa, Protein from Homo sapiens)
- Spike glycoprotein,fibritin (142 kDa, Protein from Tequatrovirus T4)
- Igg light chain - fab (22 kDa, Protein from Homo sapiens)
SARS-CoV-2 S protein bound to neutralising antibody UZGENT_A3
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8qq0
Q-score: 0.534
Acar DD, Witkowski W, Wejda M, Wei R, Desmet T, Schepens B, De Cae S, Sedeyn K, Eeckhaut H, Fijalkowska D, Roose K, Vanmarcke S, Poupon A, Jochmans D, Zhang X, Abdelnabi R, Foo CS, Weynand B, Reiter D, Callewaert N, Remaut H, Neyts J, Saelens X, Gerlo S, Vandekerckhove L
Ebiomedicine (2024) 100 pp. 104960-104960 [ DOI: doi:10.1016/j.ebiom.2023.104960 Pubmed: 38232633 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Spike glycoprotein,fibritin (142 kDa, Protein from Enterobacteria phage T4)
- Igg light chain - fab (22 kDa, Protein from Homo sapiens)
- Sars-cov2 s protein with fibritin (trimerization) tag (Complex from Severe acute respiratory syndrome coronavirus 2)
- Fab fragment of neutralising human antibody uzgent_a3 (Complex from Homo sapiens)
- Complex of sars-cov2 s protein (wuhan) and fab fragment of neutralising human antibody uzgent_a3 (Complex)
- Igg heavy chain - fab (24 kDa, Protein from Homo sapiens)
Recombinant Ena3A L-Type endospore appendages
Resolution: 3.32 Å
EM Method: Helical reconstruction
Fitted PDBs: 8pdz
Q-score: 0.42
Sleutel M, Zegeye ED, Llarena AK, Pradhan B, Fislage M, O'Sullivan K, Aspholm M, Remaut H
bioRxiv (2023) [ DOI: doi:10.1101/2023.10.23.563578 ]
Pontibacter korlensis curli subunit CsgA
Resolution: 7.6 Å
EM Method: Helical reconstruction
Fitted PDBs: 8c50
Q-score: 0.242
Structure of the GroEL chaperonin in complex with the CnoX chaperedoxin
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7ywy
Q-score: 0.393
Dupuy E, Van der Verren SE, Lin J, Wilson M, Viela F, Latour E, Dachsbeck A, Gennaris A, Vertommen D, Dufresne Y, Iorga B, Goemans CV, Remaut H, Collet JF
To Be Published
- 1:1 stoichiometric complex of groel-cnox (Complex from Escherichia coli)
- Chaperonin groel (55 kDa, Protein from Escherichia coli)
- Chaperedoxin (9 kDa, Protein from Escherichia coli)
Cryo-EM structure of undecameric SlyB from Escherichia coli K12
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7ojg
Q-score: 0.531
Janssens A, Nguyen VS, Cecil AJ, Van der Verren SE, Timmerman E, Deghelt M, Pak AJ, Collet JF, Impens F, Remaut H
Nature (2023) [ DOI: doi:10.1038/s41586-023-06925-5 ]
- Palmitic acid (256 Da, Ligand)
- Outer membrane lipoprotein slyb (15 kDa, Protein from Escherichia coli BW25113)
- Glycerol (92 Da, Ligand)
- (2~{r},4~{r},5~{r},6~{r})-6-[(1~{r})-1,2-bis(oxidanyl)ethyl]-2-[[(2~{r},3~{s},4~{r},5~{r},6~{r})-5-[[(~{e},3~{r})-3-dodecanoyloxytetradec-5-enoyl]amino]-6-[[(2~{r},3~{s},4~{r},5~{r},6~{r})-3-oxidanyl-5-[[(~{e},3~{r})-3-oxidanyltetradec-11-enoyl]amino]-4-[(~{e},3~{r})-3-oxidanyltetradec-5-enoyl]oxy-6-phosphonooxy-oxan-2-yl]methoxy]-3-phosphonooxy-4-[(~{e},3~{r})-3-tetradecanoyloxytetradec-7-enoyl]oxy-oxan-2-yl]methoxy]-4,5-bis(oxidanyl)oxane-2-carboxylic acid (2 kDa, Ligand)
- Outer membrane lipoprotein slyb (200 kDa, Complex from Escherichia coli BW25113)
- 2-(hexadecanoyloxy)-1-[(phosphonooxy)methyl]ethyl hexadecanoate (648 Da, Ligand)
Cryo-EM reconstruction of decameric SlyB from Escherichia coli K12
Janssens A, Nguyen VS, Cecil AJ, Van der Verren SE, Timmerman E, Deghelt M, Pak AJ, Collet JF, Impens F, Remaut H
Nature (2023) [ DOI: doi:10.1038/s41586-023-06925-5 ]
Cryo-EM reconstruction of dodecameric SlyB from Escherichia coli K12
Janssens A, Nguyen VS, Cecil AJ, Van der Verren SE, Timmerman E, Deghelt M, Pak AJ, Collet JF, Impens F, Remaut H
Nature (2023) [ DOI: doi:10.1038/s41586-023-06925-5 ]
Cryo-EM structure of tridecameric SlyB from Escherichia coli K12
Janssens A, Nguyen VS, Cecil AJ, Van der Verren SE, Timmerman E, Deghelt M, Pak AJ, Collet JF, Impens F, Remaut H
Nature (2023) [ DOI: doi:10.1038/s41586-023-06925-5 ]