Type I-C Cas4-Cas1-Cas2 complex bound to a PAM/PAM prespacer
Resolution: 3.49 Å
EM Method: Single-particle
Fitted PDBs: 8d3l
Q-score: 0.444
Dhingra Y, Suresh SK, Juneja P, Sashital DG
Mol Cell (2022) 82 pp. 4353- [ DOI: doi:10.1016/j.molcel.2022.09.030 Pubmed: 36272411 ]
- Iron/sulfur cluster (351 Da, Ligand)
- Manganese (ii) ion (54 Da, Ligand)
- Water (18 Da, Ligand)
- Pam/pam strand 1 (9 kDa, DNA from synthetic construct)
- Crispr-associated exonuclease cas4 (25 kDa, Protein from Alkalihalobacillus halodurans C-125)
- Crispr-associated endonuclease cas2 (11 kDa, Protein from Alkalihalobacillus halodurans C-125)
- Crispr-associated endonuclease cas1 (39 kDa, Protein from Alkalihalobacillus halodurans C-125)
- Pam/pam strand 2 (10 kDa, DNA from synthetic construct)
- Type i-c cas4-cas1-cas2 complex with a pam/pam substrate (Complex from Alkalihalobacillus halodurans C-125)
Type I-C Cas4-Cas1-Cas2 complex bound to a PAM/Processed prespacer
Resolution: 3.41 Å
EM Method: Single-particle
Fitted PDBs: 8d3m
Q-score: 0.449
Dhingra Y, Suresh SK, Juneja P, Sashital DG
Mol Cell (2022) 82 pp. 4353- [ Pubmed: 36272411 DOI: doi:10.1016/j.molcel.2022.09.030 ]
- Manganese (ii) ion (54 Da, Ligand)
- Crispr-associated endonuclease cas2 (11 kDa, Protein from Alkalihalobacillus halodurans C-125)
- Crispr-associated endonuclease cas1 (39 kDa, Protein from Alkalihalobacillus halodurans C-125)
- Crispr-associated exonuclease cas4 (25 kDa, Protein from Alkalihalobacillus halodurans C-125)
- Iron/sulfur cluster (351 Da, Ligand)
- Type i-c cas4-cas1-cas2 complex with a pam/processed substrate (Complex from Alkalihalobacillus halodurans C-125)
- Pam/processed strand 2 (10 kDa, DNA from synthetic construct)
- Pam/processed strand 1 (9 kDa, DNA from synthetic construct)
Type I-C Cas4-Cas1-Cas2 complex bound to half-site integration intermediate (HSI)
Resolution: 4.26 Å
EM Method: Single-particle
Fitted PDBs: 8d3p
Q-score: 0.278
Dhingra Y, Suresh SK, Juneja P, Sashital DG
Mol Cell (2022) 82 pp. 4353- [ DOI: doi:10.1016/j.molcel.2022.09.030 Pubmed: 36272411 ]
- Hsi strand 2- integrated prespacer strand plus repeat (17 kDa, DNA from synthetic construct)
- Iron/sulfur cluster (351 Da, Ligand)
- Hsi strand 3 bottom strand leader-repeat (12 kDa, DNA from synthetic construct)
- Type i-c cas4-cas1-cas2 complex with a pam/processed substrate (Complex from Alkalihalobacillus halodurans C-125)
- Crispr-associated endonuclease cas2 (11 kDa, Protein from Alkalihalobacillus halodurans C-125)
- Crispr-associated endonuclease cas1 (39 kDa, Protein from Alkalihalobacillus halodurans C-125)
- Hsi strand 4 top leader strand (3 kDa, DNA from synthetic construct)
- Crispr-associated exonuclease cas4 (25 kDa, Protein from Alkalihalobacillus halodurans C-125)
- Manganese (ii) ion (54 Da, Ligand)
- Hsi strand 2 (11 kDa, DNA from synthetic construct)
Type I-C Cas4-Cas1-Cas2 complex bound to a PAM/NoPAM prespacer
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 8d3q
Q-score: 0.257
Dhingra Y, Suresh SK, Juneja P, Sashital DG
Mol Cell (2022) 82 pp. 4353- [ Pubmed: 36272411 DOI: doi:10.1016/j.molcel.2022.09.030 ]
- Iron/sulfur cluster (351 Da, Ligand)
- Crispr-associated endonuclease cas1 (39 kDa, Protein from Alkalihalobacillus halodurans C-125)
- Manganese (ii) ion (54 Da, Ligand)
- Pam/nopam strand 2 (8 kDa, DNA from synthetic construct)
- Type i-c cas4-cas1-cas2 complex with a pam/processed substrate (Complex from Alkalihalobacillus halodurans C-125)
- Pam/nopam strand 1 (9 kDa, DNA from synthetic construct)
- Crispr-associated endonuclease cas2 (11 kDa, Protein from Alkalihalobacillus halodurans C-125)
- Crispr-associated exonuclease cas4 (25 kDa, Protein from Alkalihalobacillus halodurans C-125)
Type I-C Cas4-Cas1-Cas2 complex bound to a 24 bp duplex PAM/PAM prespacer
Dhingra Y, Suresh SK, Juneja P, Sashital DG
Mol Cell (2022) 82 pp. 4353-4367.e6 [ DOI: doi:10.1016/j.molcel.2022.09.030 Pubmed: 36272411 ]
Bacillus halodurans Cas1-Cas2 complex in presence of target CRISPR DNA
eLife (2019) 8 [ DOI: doi:10.7554/eLife.44248 Pubmed: 31021314 ]
- Hairpin crispr target (DNA from Bacillus halodurans C-125)
- Cas2 (Protein from Bacillus halodurans C-125)
- Cas1 (Protein from Bacillus halodurans C-125)
- Bacillus halodurans cas1-cas2 complex with target crispr hairpin dna (240 kDa, Cellular component from Bacillus halodurans C-125)
Bacillus halodurans Cas4-Cas1-Cas2 asymmetrical complex in presence of target CRISPR DNA
eLife (2019) 8 [ DOI: doi:10.7554/eLife.44248 Pubmed: 31021314 ]
- Cas1 (Protein from Bacillus halodurans C-125)
- Bacillus halodurans cas4-cas1-cas2 asymmetrical complex with target crispr hairpin dna (260 kDa, Cellular component from Bacillus halodurans C-125)
- Cas4 (Protein from Bacillus halodurans C-125)
- Cas2 (Protein from Bacillus halodurans C-125)
- Target crispr hairpin (DNA from Bacillus halodurans C-125)
Bacillus halodurans Cas4-Cas1-Cas2 symmetrical complex in presence of target CRISPR DNA
eLife (2019) 8 [ DOI: doi:10.7554/eLife.44248 Pubmed: 31021314 ]
- Bacillus halodurans cas4-cas1-cas2 symmetrical complex with target crispr hairpin dna (280 kDa, Cellular component from Bacillus halodurans C-125)
- Cas1 (Protein from Bacillus halodurans C-125)
- Cas4 (Protein from Bacillus halodurans C-125)
- Cas2 (Protein from Bacillus halodurans C-125)
- Target crispr hairpin dna (DNA from Bacillus halodurans C-125)
Bacillus halodurans Cas4-Cas1-Cas2 asymmetrical complex in presence of prespacer DNA
eLife (2019) 8 [ DOI: doi:10.7554/eLife.44248 Pubmed: 31021314 ]
- Cas1 (Protein from Bacillus halodurans C-125)
- Prespacer top strand (DNA from unidentified)
- Prespacer bottom strand (DNA from unidentified)
- Bacillus halodurans cas4-cas1-cas2 asymmetrical complex with prespacer dna (260 kDa, Cellular component from Bacillus halodurans C-125)
- Cas4 (Protein from Bacillus halodurans C-125)
- Cas2 (Protein from Bacillus halodurans C-125)
Bacillus halodurans Cas4-Cas1-Cas2 symmetrical complex in presence of prespacer DNA
eLife (2019) 8 [ DOI: doi:10.7554/eLife.44248 Pubmed: 31021314 ]
- Prespacer bottom strand (DNA from unidentified)
- Cas1 (Protein from Bacillus halodurans C-125)
- Bacillus halodurans cas4-cas1-cas2 symmetrical complex with prespacer dna (260 kDa, Cellular component from Bacillus halodurans C-125)
- Cas4 (Protein from Bacillus halodurans C-125)
- Prespacer top strand (DNA from unidentified)
- Cas2 (Protein from Bacillus halodurans C-125)