5R-MAP4, kinesin-1, and microtubule complex
Shigematsu H, Imasaki T, Doki C, Sumi T, Aoki M, Uchikubo-Kamo T, Sakamoto A, Tokuraku K, Shirouzu M, Nitta R
J Cell Biol (2018) 217 pp. 4155-4163 [ Pubmed: 30275105 DOI: doi:10.1083/jcb.201711182 ]
4R-MAP4, kinesin-1, and microtubule complex
Shigematsu H, Imasaki T, Doki C, Sumi T, Aoki M, Uchikubo-Kamo T, Sakamoto A, Tokuraku K, Shirouzu M, Nitta R
J Cell Biol (2018) 217 pp. 4155-4163 [ Pubmed: 30275105 DOI: doi:10.1083/jcb.201711182 ]
GDP-microtubule complexed with nucleotide-free KIF5C
Resolution: 5.35 Å
EM Method: Helical reconstruction
Fitted PDBs: 5xxt
Q-score: 0.182
Shima T, Morikawa M, Kaneshiro J, Kambara T, Kamimura S, Yagi T, Iwamoto H, Uemura S, Shigematsu H, Shirouzu M, Ichimura T, Watanabe TM, Nitta R, Okada Y, Hirokawa N
J Cell Biol (2018) 217 pp. 4164-4183 [ DOI: doi:10.1083/jcb.201711178 Pubmed: 30297389 ]
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Gdp-microtubule complexed with nucleotide-free kif5c (Cellular component from Mus musculus)
- Water (18 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Tubulin alpha-1a chain (48 kDa, Protein from Pig)
- Tubulin beta chain (47 kDa, Protein from Pig)
GDP-microtubule complexed with KIF5C in AMPPNP state
Resolution: 6.46 Å
EM Method: Helical reconstruction
Fitted PDBs: 5xxv
Q-score: 0.149
Shima T, Morikawa M, Kaneshiro J, Kambara T, Kamimura S, Yagi T, Iwamoto H, Uemura S, Shigematsu H, Shirouzu M, Ichimura T, Watanabe TM, Nitta R, Okada Y, Hirokawa N
J Cell Biol (2018) 217 pp. 4164-4183 [ DOI: doi:10.1083/jcb.201711178 Pubmed: 30297389 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Gdp-microtubule complexed with kif5c in amppnp state (Cellular component from Mus musculus)
- Tubulin alpha-1a chain (48 kDa, Protein from Pig)
- Water (18 Da, Ligand)
- Tubulin beta chain (47 kDa, Protein from Pig)
- Guanosine-5'-triphosphate (523 Da, Ligand)
GDP-microtubule complexed with KIF5C in ATP state
Resolution: 6.0 Å
EM Method: Helical reconstruction
Fitted PDBs: 5xxw
Q-score: 0.164
Shima T, Morikawa M, Kaneshiro J, Kambara T, Kamimura S, Yagi T, Iwamoto H, Uemura S, Shigematsu H, Shirouzu M, Ichimura T, Watanabe TM, Nitta R, Okada Y, Hirokawa N
J Cell Biol (2018) 217 pp. 4164-4183 [ DOI: doi:10.1083/jcb.201711178 Pubmed: 30297389 ]
- Gdp-microtubule complexed with kif5c in atp state (Cellular component from Mus musculus)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Water (18 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Tubulin alpha-1a chain (48 kDa, Protein from Pig)
- Tubulin beta chain (47 kDa, Protein from Pig)
GMPCPP-microtubule complexed with nucleotide-free KIF5C
Resolution: 6.43 Å
EM Method: Helical reconstruction
Fitted PDBs: 5xxx
Q-score: 0.154
Shima T, Morikawa M, Kaneshiro J, Kambara T, Kamimura S, Yagi T, Iwamoto H, Uemura S, Shigematsu H, Shirouzu M, Ichimura T, Watanabe TM, Nitta R, Okada Y, Hirokawa N
J Cell Biol (2018) 217 pp. 4164-4183 [ DOI: doi:10.1083/jcb.201711178 Pubmed: 30297389 ]
- Magnesium ion (24 Da, Ligand)
- Tubulin alpha-1a chain (48 kDa, Protein from Pig)
- Water (18 Da, Ligand)
- Tubulin beta chain (47 kDa, Protein from Pig)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Gmpcpp-microtubule complexed with nucleotide-free kif5c (Cellular component from Mus musculus)
Kinesin-8 motor, KIF19A, in the nucleotide-free state complexed with GDP-taxol microtubule
Resolution: 7.0 Å
EM Method: Helical reconstruction
Fitted PDBs: 5gsy
Q-score: 0.085
Wang D, Nitta R, Morikawa M, Yajima H, Inoue S, Shigematsu H, Kikkawa M, Hirokawa N
eLife (2016) 5 [ DOI: doi:10.7554/eLife.18101 Pubmed: 27690357 ]
Structural basis of backwards motion in kinesin-14: minus-end directed nKn664 in the AMPPNP state
Resolution: 6.6 Å
EM Method: Helical reconstruction
Fitted PDBs: 5hnw
Q-score: 0.139
Yamagishi M, Shigematsu H, Yokoyama T, Kikkawa M, Sugawa M, Aoki M, Shirouzu M, Yajima J, Nitta R
Structure (2016) 24 pp. 1322-1334 [ DOI: doi:10.1016/j.str.2016.05.021 Pubmed: 27452403 ]
Resolution: 6.6 Å
EM Method: Helical reconstruction
Fitted PDBs: 5hnx
Q-score: 0.138
Yamagishi M, Shigematsu H, Yokoyama T, Kikkawa M, Sugawa M, Aoki M, Shirouzu M, Yajima J, Nitta R
Structure (2016) 24 pp. 1322-1334 [ DOI: doi:10.1016/j.str.2016.05.021 Pubmed: 27452403 ]
- Taxol (853 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Tubulin alpha-1b chain (50 kDa, Protein from Bos taurus)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Protein claret segregational,kinesin-1/kinesin-14,protein claret segregational (Complex)
- Minus-end directed ncd chimera nkn664 in the amppnp state complexed with gdp-taxol microtubule (Complex)
- Tubulin beta-2b chain (Complex from Bos taurus)
- Protein claret segregational,kinesin-1/kinesin-14,protein claret segregational (41 kDa, Protein from Drosophila melanogaster)
- Tubulin beta-2b chain (49 kDa, Protein from Bos taurus)
- Tubulin alpha-1b chain (Complex from Bos taurus)
Structural basis of backwards motion in kinesin-14: plus-end directed nKn669 in the AMPPNP state
Resolution: 6.3 Å
EM Method: Helical reconstruction
Fitted PDBs: 5hny
Q-score: 0.145
Yamagishi M, Shigematsu H, Yokoyama T, Kikkawa M, Sugawa M, Aoki M, Shirouzu M, Yajima J, Nitta R
Structure (2016) 24 pp. 1322-1334 [ Pubmed: 27452403 DOI: doi:10.1016/j.str.2016.05.021 ]
- Tubulin beta-2b chain (47 kDa, Protein from Bovine)
- Taxol (853 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Tubulin beta-2b chain (Complex)
- Tubulin alpha-1b chain (Complex)
- Plus-end directed ncd chimera nkn669 in the amppnp state complexed with gdp-taxol microtubule (Complex)
- Tubulin alpha-1b chain (48 kDa, Protein from Bovine)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Protein claret segregational, kinesin-1/kinesin-14, protein claret segregational (41 kDa, Protein from Drosophila melanogaster)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Protein claret segregational,plus-end directed kinesin-1/kinesin-14,protein claret segregational (Complex)