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EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Research:

perform basic research in molecular biology

Services:

offer vital services to scientists in the member states

Training

scientists, students and visitors at all levels

Transfer

and development of technology

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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primary_citation_author:"Shigematsu H" AND author_name:"Shigematsu H" AND resolution:[3 TO 4} AND database:"EMDB" AND author_orcid:"0000-0002-8778-8261"

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EMD-33293

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Cryo-EM structure of Cytochrome bo3 from Escherichia coli, apo structure with DMSO

Release date: Dec. 21, 2022
Resolution: 3.09 Å
EM Method: Single-particle
Fitted PDBs: 7xmc
Q-score: 0.526

Nishida Y, Yanagisawa S, Morita R, Shigematsu H, Shinzawa-Itoh K, Yuki H, Ogasawara S, Shimuta K, Iwamoto T, Nakabayashi C, Matsumura W, Kato H, Gopalasingam C, Nagao T, Qaqorh T, Takahashi Y, Yamazaki S, Kamiya K, Harada R, Mizuno N, Takahashi H, Akeda Y, Ohnishi M, Ishii Y, Kumasaka T, Murata T, Muramoto K, Tosha T, Shiro Y, Honma T, Shigeta Y, Kubo M, Takashima S, Shintani Y

Nat Commun (2022) 13 pp. 7591-7591 [ Pubmed: 36481732 DOI: doi:10.1038/s41467-022-34771-y ]

Sample:

Heme o (838 Da, Ligand)
Copper (ii) ion (63 Da, Ligand)
Ubiquinol oxidase subunit 2 (36 kDa, Protein from Escherichia coli)
Cytochrome bo(3) ubiquinol oxidase subunit 1 (74 kDa, Protein from Escherichia coli)
Cytochrome bo3 ubiquinol oxidase (144 kDa, Complex from Escherichia coli)
Cytochrome bo(3) ubiquinol oxidase subunit 4 (12 kDa, Protein from Escherichia coli)
Cytochrome bo(3) ubiquinol oxidase subunit 3 (22 kDa, Protein from Escherichia coli)
Unknown atom or ion (744 Da, Ligand)
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
Protoporphyrin ix containing fe (616 Da, Ligand)

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