Wheat 80S ribosome pausing on AUG-Stop with cycloheximide
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8ipb
Q-score: 0.466
Tanaka M, Yokoyama T, Saito H, Nishimoto M, Tsuda K, Sotta N, Shigematsu H, Shirouzu M, Iwasaki S, Ito T, Fujiwara T
Nat Chem Biol (2024) 20 pp. 605-614 [ DOI: doi:10.1038/s41589-023-01513-0 Pubmed: 38267667 ]
- 60s ribosomal protein ul30 (28 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein el40 (14 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein el14 (15 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein ul22 (19 kDa, Protein from Triticum aestivum)
- 40s ribosomal protein es6 (28 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein el24 (18 kDa, Protein from Triticum aestivum)
- 40s ribosomal protein us3 (25 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein ul29 (14 kDa, Protein from Triticum aestivum)
- 40s ribosomal protein es25 (12 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein ul23 (16 kDa, Protein from Triticum aestivum)
- 40s ribosomal protein es30 (6 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein el33 (12 kDa, Protein from Triticum aestivum)
- 40s ribosomal protein us10 (14 kDa, Protein from Triticum aestivum)
- Rack1 (36 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein el34 (13 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein el13 (24 kDa, Protein from Triticum aestivum)
- 60s ribosomal rna (1 MDa, RNA from Triticum aestivum)
- 60s ribosomal protein el27 (15 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein el39 (9 kDa, Protein from Triticum aestivum)
- 40s ribosomal protein us17 (17 kDa, Protein from Triticum aestivum)
- 5s ribosomal rna (38 kDa, RNA from Triticum aestivum)
- 60s ribosomal protein ul6 (21 kDa, Protein from Triticum aestivum)
- 40s ribosomal protein es4 (30 kDa, Protein from Triticum aestivum)
- 40s ribosomal protein us7 (22 kDa, Protein from Triticum aestivum)
- 4-{(2r)-2-[(1s,3s,5s)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione (281 Da, Ligand)
- 40s ribosomal protein us14 (6 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein el29 (6 kDa, Protein from Triticum aestivum)
- 40s ribosomal protein es28 (9 kDa, Protein from Triticum aestivum)
- 40s ribosomal protein es26 (16 kDa, Protein from Triticum aestivum)
- 40s ribosomal protein us5 (29 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein el6 (24 kDa, Protein from Triticum aestivum)
- 40s ribosomal protein es8 (25 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein el38 (8 kDa, Protein from Triticum aestivum)
- Mrna (2 kDa, RNA from Triticum aestivum)
- 60s ribosomal protein ul15 (16 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein el43 (10 kDa, Protein from Triticum aestivum)
- 40s ribosomal protein us13 (17 kDa, Protein from Triticum aestivum)
- 40s ribosomal protein us12 (15 kDa, Protein from Triticum aestivum)
- 40s ribosomal protein es19 (17 kDa, Protein from Triticum aestivum)
- Trnai (24 kDa, RNA from Triticum aestivum)
- 60s ribosomal protein ul14 (15 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein ul3 (44 kDa, Protein from Triticum aestivum)
- 40s ribosomal protein es24 (15 kDa, Protein from Triticum aestivum)
- Wheat 80s ribosome pausing on aug-stop with cycloheximide (Complex from Triticum aestivum)
- 60s ribosomal protein el20 (21 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein ul4 (44 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein el30 (12 kDa, Protein from Triticum aestivum)
- 40s ribosomal protein us2 (33 kDa, Protein from Triticum aestivum)
- 5.8s ribosomal rna (51 kDa, RNA from Triticum aestivum)
- 40s ribosomal protein us11 (16 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein ul24 (17 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein el41 (3 kDa, Protein from Oryza sativa)
- 40s ribosomal protein us19 (17 kDa, Protein from Triticum aestivum)
- 40s ribosomal protein es1 (30 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein el18 (21 kDa, Protein from Triticum aestivum)
- Zinc ion (65 Da, Ligand)
- 40s ribosomal protein us4 (22 kDa, Protein from Triticum aestivum)
- 40s ribosomal protein us8 (14 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein ul18 (34 kDa, Protein from Triticum aestivum)
- 40s ribosomal protein us9 (16 kDa, Protein from Triticum aestivum)
- 40s ribosomal protein us15 (17 kDa, Protein from Triticum aestivum)
- 40s ribosomal protein es27 (9 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein el19 (24 kDa, Protein from Triticum aestivum)
- 40s ribosomal protein es17 (16 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein el32 (15 kDa, Protein from Triticum aestivum)
- 40s ribosomal protein es21 (9 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein ul2 (28 kDa, Protein from Triticum aestivum)
- 40s ribosomal protein es10 (20 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein ul13 (23 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein el22 (14 kDa, Protein from Triticum aestivum)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein el42 (12 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein ul16 (24 kDa, Protein from Triticum aestivum)
- 18s ribosomal rna (583 kDa, RNA from Triticum aestivum)
- 40s ribosomal protein es7 (22 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein el37 (10 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein ul5 (20 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein el36 (12 kDa, Protein from Triticum aestivum)
- 40s ribosomal protein el8 (29 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein el21 (18 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein el31 (14 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein el15 (24 kDa, Protein from Triticum aestivum)
- 60s ribosomal protein el28 (16 kDa, Protein from Triticum aestivum)
Cryo-EM structure of tetrameric TLR3 in complex with dsRNA (90 bp)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7wm4
Q-score: 0.256
Sakaniwa K, Fujimura A, Shibata T, Shigematsu H, Ekimoto T, Yamamoto M, Ikeguchi M, Miyake K, Ohto U, Shimizu T
Nat Commun (2023) 14 pp. 164-164 [ DOI: doi:10.1038/s41467-023-35844-2 Pubmed: 36631495 ]
- Rna (81-mer) (25 kDa, RNA from synthetic construct)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Toll-like receptor 3 (77 kDa, Protein from Mus musculus)
- Rna (81-mer) (25 kDa, RNA from synthetic construct)
- Tetrameric tlr3 in complex with dsrna (90 bp) (Complex from Mus musculus)
Cryo-EM structure of Cytochrome bo3 from Escherichia coli, apo structure with DMSO
Resolution: 3.09 Å
EM Method: Single-particle
Fitted PDBs: 7xmc
Q-score: 0.526
Nishida Y, Yanagisawa S, Morita R, Shigematsu H, Shinzawa-Itoh K, Yuki H, Ogasawara S, Shimuta K, Iwamoto T, Nakabayashi C, Matsumura W, Kato H, Gopalasingam C, Nagao T, Qaqorh T, Takahashi Y, Yamazaki S, Kamiya K, Harada R, Mizuno N, Takahashi H, Akeda Y, Ohnishi M, Ishii Y, Kumasaka T, Murata T, Muramoto K, Tosha T, Shiro Y, Honma T, Shigeta Y, Kubo M, Takashima S, Shintani Y
Nat Commun (2022) 13 pp. 7591-7591 [ Pubmed: 36481732 DOI: doi:10.1038/s41467-022-34771-y ]
- Heme o (838 Da, Ligand)
- Copper (ii) ion (63 Da, Ligand)
- Ubiquinol oxidase subunit 2 (36 kDa, Protein from Escherichia coli)
- Cytochrome bo(3) ubiquinol oxidase subunit 1 (74 kDa, Protein from Escherichia coli)
- Cytochrome bo3 ubiquinol oxidase (144 kDa, Complex from Escherichia coli)
- Cytochrome bo(3) ubiquinol oxidase subunit 4 (12 kDa, Protein from Escherichia coli)
- Cytochrome bo(3) ubiquinol oxidase subunit 3 (22 kDa, Protein from Escherichia coli)
- Unknown atom or ion (744 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Protoporphyrin ix containing fe (616 Da, Ligand)
Cryo-EM structure of human TLR3 in complex with UNC93B1
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7c76
Q-score: 0.437
Ishida H, Asami J, Zhang Z, Nishizawa T, Shigematsu H, Ohto U, Shimizu T
Nat Struct Mol Biol (2021) 28 pp. 173-180 [ Pubmed: 33432245 DOI: doi:10.1038/s41594-020-00542-w ]
- Toll-like receptor 3 (103 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Protein unc-93 homolog b1 (66 kDa, Protein from Homo sapiens)
- Complex of tlr3 and unc93b1 (Complex from Homo sapiens)
Cryo-EM structure of mouse TLR3 in complex with UNC93B1
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7c77
Q-score: 0.424
Ishida H, Asami J, Zhang Z, Nishizawa T, Shigematsu H, Ohto U, Shimizu T
Nat Struct Mol Biol (2021) 28 pp. 173-180 [ Pubmed: 33432245 DOI: doi:10.1038/s41594-020-00542-w ]
- Toll-like receptor 3 (103 kDa, Protein from Mus musculus)
- Protein unc-93 homolog b1 (67 kDa, Protein from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Complex of tlr3 and unc93b1 (Complex from Mus musculoides)
Cryo-EM Structure of the Prostaglandin E Receptor EP4 Coupled to G Protein
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7d7m
Q-score: 0.47
Nojima S, Fujita Y, Kimura KT, Nomura N, Suno R, Morimoto K, Yamamoto M, Noda T, Iwata S, Shigematsu H, Kobayashi T
Structure (2021) 29 pp. 252-260.e6 [ DOI: doi:10.1016/j.str.2020.11.007 Pubmed: 33264604 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,guanine nucleotide-binding protein g(s) subunit alpha isoforms short,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (29 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Prostaglandin e2 receptor ep4 subtype,guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1,guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Nanobody nb35 (14 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody nb35 (Complex from Lama glama)
- Pge2-ep4-gs-gbeta1-ggamma2-nb35 complex (120 kDa, Complex)
- Prostaglandin e2 receptor ep4 subtype,prostaglandin e2 receptor ep4 subtype (36 kDa, Protein from Homo sapiens)
- (z)-7-[(1r,2r,3r)-3-hydroxy-2-[(e,3s)-3-hydroxyoct-1-enyl]-5-oxo-cyclopentyl]hept-5-enoic acid (352 Da, Ligand)
RNA Polymerase II elongation complex bound with Spt4/5 and TFIIS
Resolution: 3.83 Å
EM Method: Single-particle
Fitted PDBs: 5xon
Q-score: 0.336
Ehara H, Yokoyama T, Shigematsu H, Yokoyama S, Shirouzu M, Sekine SI
Science (2017) 357 pp. 921-924 [ Pubmed: 28775211 DOI: doi:10.1126/science.aan8552 ]
- Rna polymerase subunit abc10-beta, common to rna polymerases i, ii, and iii (8 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Dna (48-mer) (14 kDa, DNA from synthetic construct)
- Rna polymerase ii subunit (18 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Rna polymerase ii (Complex)
- Rna polymerase subunit abc27, common to rna polymerases i, ii, and iii (24 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Rna (30-mer) (9 kDa, RNA from synthetic construct)
- General transcription elongation factor tfiis (21 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Protein that forms a complex with spt4p (68 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Magnesium ion (24 Da, Ligand)
- Rna polymerase subunit abc23, common to rna polymerases i, ii, and iii (17 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Transcription elongation factor spt4 (12 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Rna polymerase ii elongation complex bound with spt4/5 and tfiis (Complex from Komagataella pastoris)
- Rna polymerase subunit, found in rna polymerase complexes i, ii, and iii (7 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Rna polymerase ii subunit b12.5 (13 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Rna polymerase subunit abc14.5, common to rna polymerases i, ii, and iii (16 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Dna (48-mer) (14 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Rna polymerase ii third largest subunit b44, part of central core (34 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Rna polymerase ii subunit b32 (20 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Spt4/5 and tfiis (Complex)
- Dna-directed rna polymerase subunit (194 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Dna-directed rna polymerase subunit beta (139 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Dna-directed rna polymerase subunit (13 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))