Electron Microscopy Data Bank

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RNA Polymerase II elongation complex bound with Spt4/5 and TFIIS

Release date: Aug. 16, 2017
Resolution: 3.83 Å
EM Method: Single-particle
Fitted PDBs: 5xon
Q-score: 0.336

Ehara H, Yokoyama T, Shigematsu H, Yokoyama S, Shirouzu M, Sekine SI

Science (2017) 357 pp. 921-924 [ Pubmed: 28775211 DOI: doi:10.1126/science.aan8552 ]

Sample:

Rna polymerase subunit abc10-beta, common to rna polymerases i, ii, and iii (8 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
Dna (48-mer) (14 kDa, DNA from synthetic construct)
Rna polymerase ii subunit (18 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
Rna polymerase ii (Complex)
Rna polymerase subunit abc27, common to rna polymerases i, ii, and iii (24 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
Rna (30-mer) (9 kDa, RNA from synthetic construct)
General transcription elongation factor tfiis (21 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
Protein that forms a complex with spt4p (68 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
Magnesium ion (24 Da, Ligand)
Rna polymerase subunit abc23, common to rna polymerases i, ii, and iii (17 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
Transcription elongation factor spt4 (12 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
Rna polymerase ii elongation complex bound with spt4/5 and tfiis (Complex from Komagataella pastoris)
Rna polymerase subunit, found in rna polymerase complexes i, ii, and iii (7 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
Rna polymerase ii subunit b12.5 (13 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
Rna polymerase subunit abc14.5, common to rna polymerases i, ii, and iii (16 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
Dna (48-mer) (14 kDa, DNA from synthetic construct)
Zinc ion (65 Da, Ligand)
Rna polymerase ii third largest subunit b44, part of central core (34 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
Rna polymerase ii subunit b32 (20 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
Spt4/5 and tfiis (Complex)
Dna-directed rna polymerase subunit (194 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
Dna-directed rna polymerase subunit beta (139 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
Dna-directed rna polymerase subunit (13 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))

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