Lehman LC, Bacic L, Hewitt G, Brackmann K, Sabantsev A, Gaullier G, Pytharopoulou S, Degliesposti G, Okkenhaug H, Tan S, Costa A, Skehel JM, Boulton SJ, Deindl S
Cell Rep (2020) 33 [ Pubmed: 33357431 DOI: 10.1016/j.celrep.2020.108529 ]
Image sets:
- Particles of the high-resolution nucleosome class
- Unaligned movies of the nucleosome - ALC1-linker complex - session_1_disk_3
- Particles of the hexasome class
- Unaligned movies of the nucleosome - ALC1-linker complex - session_2_grid_2
- Unaligned movies of the nucleosome - ALC1-linker complex - session_1_disk_2
- Unaligned movies of the nucleosome - ALC1-linker complex - session_2_grid_1
- Unaligned movies of the nucleosome - ALC1-linker complex - session_1_disk_1
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 6zhx
Q-score: 0.625
Lehmann LC, Bacic L, Hewitt G, Brackmann K, Sabantsev A, Gaullier G, Pytharopoulou S, Degliesposti G, Okkenhaug H, Tan S, Costa A, Skehel JM, Boulton SJ, Deindl S
Cell Rep (2020) 33 pp. 108529-108529 [ Pubmed: 33357431 DOI: doi:10.1016/j.celrep.2020.108529 ]
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Dna (Complex from synthetic construct)
- Histones (Complex from Xenopus laevis)
- Dna (145-mer) widom 601 sequence (44 kDa, DNA from synthetic construct)
- Chromodomain-helicase-dna-binding protein 1-like (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Chromodomain-helicase-dna-binding protein 1-like (4 kDa, Protein from Homo sapiens)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Dna (145-mer) widom 601 sequence (44 kDa, DNA from synthetic construct)
- Crosslinked complex of alc1 regulatory linker and the nucleosome (206 kDa, Complex)
- Histone h2a type 1 (14 kDa, Protein from Xenopus laevis)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6zhy
Q-score: 0.516
Lehmann LC, Bacic L, Hewitt G, Brackmann K, Sabantsev A, Gaullier G, Pytharopoulou S, Degliesposti G, Okkenhaug H, Tan S, Costa A, Skehel JM, Boulton SJ, Deindl S
Cell Rep (2020) 33 pp. 108529-108529 [ Pubmed: 33357431 DOI: doi:10.1016/j.celrep.2020.108529 ]
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Dna (110-mer) widom 601 sequence (44 kDa, DNA from synthetic construct)
- A hexasome (nucleosome lacking one of the two h2a-h2b dimers) with its acidic patch bound by the alc1 linker regulatory region. (170 kDa, Complex)
- Dna (Complex from synthetic construct)
- Histones (Complex from Xenopus laevis)
- Chromodomain-helicase-dna-binding protein 1-like (Complex from Homo sapiens)
- Dna (110-mer) widom 601 sequence (44 kDa, DNA from synthetic construct)
- Chromodomain-helicase-dna-binding protein 1-like (4 kDa, Protein from Homo sapiens)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Histone h2a type 1 (14 kDa, Protein from Xenopus laevis)