Cryo-EM structure of Cas12k-sgRNA binary complex (type V-K CRISPR-associated transposon)
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 8axb
Q-score: 0.433
Tenjo-Castano F, Sofos N, Stella S, Molina R, Pape T, Lopez-Mendez B, Stutzke LS, Temperini P, Montoya G
To Be Published
- Water (18 Da, Ligand)
- Cas12k (74 kDa, Protein from Scytonema hofmannii)
- Sgrna (84 kDa, RNA from Scytonema hofmannii)
- Binary complex of shcas12k bound to sgrna. (159 kDa, Complex from Scytonema hofmannii)
Resolution: 2.46 Å
EM Method: Single-particle
Fitted PDBs: 8aa5
Q-score: 0.519
Tenjo-Castano F, Sofos N, Lopez-Mendez B, Stutzke LS, Fuglsang A, Stella S, Montoya G
Nat Commun (2022) 13 pp. 5792-5792 [ Pubmed: 36184667 DOI: doi:10.1038/s41467-022-33504-5 ]
- Target_1 (4 kDa, DNA from Scytonema hofmannii)
- Le_target (24 kDa, DNA from Scytonema hofmannii)
- Target_2 (4 kDa, DNA from Scytonema hofmannii)
- Ternary complex of shtnsb transposase with strand transfer complex dna. (359 kDa, Complex from Scytonema hofmannii)
- Water (18 Da, Ligand)
- Re_target (24 kDa, DNA from Scytonema hofmannii)
- Le_polya (23 kDa, DNA from Scytonema hofmannii)
- Tnsb (68 kDa, Protein from Scytonema hofmannii)
- Re_polya (22 kDa, DNA from Scytonema hofmannii)
Type V-K CAST TnsB bound to LTR-SR
Tenjo-Castano F, Sofos N, Lopez-Mendez B, Stutzke LS, Fuglsang A, Stella S, Montoya G
Nat Commun (2022) 13 pp. 5792-5792 [ Pubmed: 36184667 DOI: doi:10.1038/s41467-022-33504-5 ]
- Rightelement (DNA from Scytonema hofmannii)
- Type v-k cast tnsb bound to ltr-sr (359 kDa, Complex from Scytonema hofmannii)
- Tnsb (Protein from Scytonema hofmannii)
- Leftelement (DNA from Scytonema hofmannii)
Structure of the mini-RNA-guided endonuclease CRISPR-Cas_phi3
Resolution: 2.66 Å
EM Method: Single-particle
Fitted PDBs: 7odf
Q-score: 0.548
Carabias A, Fuglsang A, Temperini P, Pape T, Sofos N, Stella S, Erlendsson S, Montoya G
Nat Commun (2021) 12 pp. 4476-4476 [ DOI: doi:10.1038/s41467-021-24707-3 Pubmed: 34294706 ]
- Cas_phi/r-loop complex (140 kDa, Complex)
- Dna (5'-d(p*gp*tp*ap*ap*tp*tp*cp*ap*g)-3') (2 kDa, DNA from synthetic construct)
- Water (18 Da, Ligand)
- Dna (5'-d(p*gp*tp*ap*tp*cp*cp*cp*ap*tp*tp*ap*cp*cp*ap*gp*cp*tp*gp*ap*ap*tp*tp*ap*c)-3') (7 kDa, DNA from synthetic construct)
- Dna and rna (Complex from synthetic construct)
- Cas_phi3 (87 kDa, Protein from Phage #D)
- Dna (5'-d(p*gp*g)-3') (613 Da, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Cas_phi3 (Complex from Phage)
- Nickel (ii) ion (58 Da, Ligand)
- Rna (43-mer) (13 kDa, RNA from synthetic construct)
Type III-B Cmr-beta Cryo-EM structure of the Apo state
Resolution: 2.75 Å
EM Method: Single-particle
Fitted PDBs: 6s6b
Q-score: 0.456
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]
- Crrna (51-mer) (16 kDa, RNA from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Zinc ion (65 Da, Ligand)
- Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Cmr1 (55 kDa, Protein from Sulfolobus islandicus REY15A)
- Type iii-b cmr-beta binary complex, crrna-bound (900 kDa, Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated protein cmrx (19 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus (strain REY15A))
Cryo-EM structure of the Type III-B Cmr-beta bound to cognate target RNA and AMPPnP, state 1
Resolution: 2.41 Å
EM Method: Single-particle
Fitted PDBs: 6s8b
Q-score: 0.48
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]
- Cmr1 (55 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated protein cmrx (19 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Manganese (ii) ion (54 Da, Ligand)
- Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crrna (16 kDa, RNA from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Cognate target rna (46-mer) (14 kDa, RNA from Sulfolobus islandicus REY15A)
- Type iii-b cmr-beta ternary complex, cognate target rna and amppnp bound (900 kDa, Complex from Sulfolobus islandicus REY15A)
- Zinc ion (65 Da, Ligand)
- Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus (strain REY15A))
Cryo-EM structure of the type III-B Cmr-beta complex bound to non-cognate target RNA
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6s8e
Q-score: 0.397
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]
- Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus REY15A)
- Type iii-b cmr-beta complex bound to non-cognate target rna (900 kDa, Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus REY15A)
- Zinc ion (65 Da, Ligand)
- Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus REY15A)
- Crrna (16 kDa, RNA from Sulfolobus islandicus REY15A)
- Crispr-associated protein cmr7 (19 kDa, Protein from Sulfolobus islandicus REY15A)
- Cmr1,crispr-associated ramp protein, cmr1 family,crispr-associated ramp protein, cmr1 family (55 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus REY15A)
- Non-cognate target rna (15 kDa, RNA from synthetic construct)
Cryo-EM structure of the Type III-B Cmr-beta bound to cognate target RNA and AMPPnP, state 2
Resolution: 2.68 Å
EM Method: Single-particle
Fitted PDBs: 6s91
Q-score: 0.463
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]
- Cmr1,crispr-associated ramp protein, cmr1 family (55 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Cognate target rna (Complex from synthetic construct)
- Crrna (48-mer) (16 kDa, RNA from Sulfolobus islandicus REY15A)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Cognate target rna (14 kDa, RNA from synthetic construct)
- Manganese (ii) ion (54 Da, Ligand)
- Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated ramp proteins cmr4 and cmr6, and crrna (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein cmrx (19 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Zinc ion (65 Da, Ligand)
- Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated proteins cmr5, cmr3, cmr1, cmr2, cmr7 (Complex from Sulfolobus islandicus REY15A)
- Type iii-b cmr-beta ternary complex, cognate target rna and amppnp bound (900 kDa, Complex)
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 6sh8
Q-score: 0.437
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]
- Cmr1,crispr-associated ramp protein, cmr1 family (55 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus REY15A)
- Type iii-b cmr-beta ternary complex, cognate target rna and amppnp bound, in the presence of ssdna (900 kDa, Complex)
- Manganese (ii) ion (54 Da, Ligand)
- Cmr1,crispr-associated ramp protein, cmr1 family (Complex from Sulfolobus islandicus REY15A)
- Crrna (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr2 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr6 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr5 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus REY15A)
- Zinc ion (65 Da, Ligand)
- Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus REY15A)
- Crrna (16 kDa, RNA from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr3 family (Complex from Sulfolobus islandicus REY15A)
- Cognate target rna (Complex from synthetic construct)
- Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein cmrx (19 kDa, Protein from Sulfolobus islandicus REY15A)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Cognate target rna (14 kDa, RNA from synthetic construct)
- Crispr-associated ramp protein, cmr4 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein cmrx (Complex from Sulfolobus islandicus REY15A)
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 6shb
Q-score: 0.442
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]
- Crrna (49-mer) (Complex from Sulfolobus islandicus REY15A)
- Cmr1,crispr-associated ramp protein, cmr1 family (55 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus REY15A)
- Type iii-b cmr-beta ternary complex, cognate target rna and amppnp bound, in the presence of ssdna (900 kDa, Complex)
- Manganese (ii) ion (54 Da, Ligand)
- Cmr1,crispr-associated ramp protein, cmr1 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr2 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr6 family (Complex from Sulfolobus islandicus REY15A)
- Crrna (49-mer) (16 kDa, RNA from Sulfolobus islandicus REY15A)
- Cognate target rna (43-mer) (Complex from synthetic construct)
- Crispr-associated protein, cmr5 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus REY15A)
- Zinc ion (65 Da, Ligand)
- Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr3 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus REY15A)
- Cognate target rna (43-mer) (14 kDa, RNA from synthetic construct)
- Crispr-associated protein cmrx (19 kDa, Protein from Sulfolobus islandicus REY15A)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Crispr-associated ramp protein, cmr4 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein cmrx (Complex from Sulfolobus islandicus REY15A)