Resolution: 2.74 Å
EM Method: Single-particle
Fitted PDBs: 8smv
Q-score: 0.572
Hoppe N, Harrison S, Hwang SH, Chen Z, Karelina M, Deshpande I, Suomivuori CM, Palicharla VR, Berry SP, Tschaikner P, Regele D, Covey DF, Stefan E, Marks DS, Reiter J, Dror RO, Evers AS, Mukhopadhyay S, Manglik A
bioRxiv (2023) [ Pubmed: 37292845 DOI: doi:10.1101/2023.05.23.540554 ]
- Nanobody 35 (Complex from Lama glama)
- G-protein coupled receptor 161 (59 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
- Gpr161 gs heterotrimeric complex with nb35 (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 161, guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Cannabinoid Receptor 1-G Protein Complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8ghv
Q-score: 0.569
Krishna Kumar K, Robertson MJ, Thadhani E, Wang H, Suomivuori CM, Powers AS, Ji L, Nikas SP, Dror RO, Inoue A, Makriyannis A, Skiniotis G, Kobilka B
Nat Commun (2023) 14 pp. 2672-2672 [ DOI: doi:10.1038/s41467-023-37864-4 Pubmed: 37160876 ]
- Scfv16 (Complex from Mus musculus)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Gi g protein heterotrimer (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Cannabinoid receptor 1 in complex with gi protein heterotrimer and scfv16 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cannabinoid receptor 1 (Complex from Homo sapiens)
- Cannabinoid receptor 1 (55 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- (5z,8z,11z,13s,14z)-n-[(2r)-1-hydroxypropan-2-yl]-13-methylicosa-5,8,11,14-tetraenamide (375 Da, Ligand)
Cannabinoid Receptor 1/G protein complex, Global Refinement
Krishna Kumar K, Robertson MJ, Thadhani E, Wang H, Suomivuori CM, Powers AS, Ji L, Nikas SP, Dror RO, Inoue A, Makriyannis A, Skiniotis G, Kobilka B
Nat Commun (2023) 14 pp. 2672-2672 [ DOI: doi:10.1038/s41467-023-37864-4 Pubmed: 37160876 ]
Cannabinoid Receptor 1/G protein complex, local refinement
Krishna Kumar K, Robertson MJ, Thadhani E, Wang H, Suomivuori CM, Powers AS, Ji L, Nikas SP, Dror RO, Inoue A, Makriyannis A, Skiniotis G, Kobilka B
Nat Commun (2023) 14 pp. 2672-2672 [ DOI: doi:10.1038/s41467-023-37864-4 Pubmed: 37160876 ]
CryoEM structure of mu-opioid receptor - Gi protein complex bound to mitragynine pseudoindoxyl (MP)
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7t2g
Q-score: 0.672
Qu Q, Huang W, Aydin D, Paggi JM, Seven AB, Wang H, Chakraborty S, Che T, DiBerto JF, Robertson MJ, Inoue A, Suomivuori CM, Roth BL, Majumdar S, Dror RO, Kobilka BK, Skiniotis G
Nat Chem Biol (2022) [ DOI: doi:10.1038/s41589-022-01208-y Pubmed: 36411392 ]
- Mitragynine pseudoindoxyl (414 Da, Ligand)
- Mu-opioid receptor / gi protein complex bound to mitragynine pseudoindoxyl (mp). (Complex from Mus musculus)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Soluble cytochrome b562,mu-type opioid receptor (55 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
CryoEM structure of mu-opioid receptor - Gi protein complex bound to lofentanil (LFT)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7t2h
Q-score: 0.528
Qu Q, Huang W, Aydin D, Paggi JM, Seven AB, Wang H, Chakraborty S, Che T, DiBerto JF, Robertson MJ, Inoue A, Suomivuori CM, Roth BL, Majumdar S, Dror RO, Kobilka BK, Skiniotis G
Nat Chem Biol (2022) [ Pubmed: 36411392 DOI: doi:10.1038/s41589-022-01208-y ]
- Scfv16 (27 kDa, Protein from Mus musculus)
- Lofentanil (408 Da, Ligand)
- Mu-type opioid receptor (39 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Mu-opioid receptor - gi protein complex bound to lofentanil (lft) (Complex from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
5-HT2B receptor bound to LSD obtained by cryo-electron microscopy (cryoEM)
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7srq
Q-score: 0.446
Cao C, Barros-Alvarez X, Zhang S, Kim K, Damgen MA, Panova O, Suomivuori CM, Fay JF, Zhong X, Krumm BE, Gumpper RH, Seven AB, Robertson MJ, Krogan NJ, Huttenhain R, Nichols DE, Dror RO, Skiniotis G, Roth BL
Neuron (2022) 110 pp. 3154- [ DOI: doi:10.1016/j.neuron.2022.08.006 Pubmed: 36087581 ]
- 5-hydroxytryptamine receptor 2b (35 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- (8alpha)-n,n-diethyl-6-methyl-9,10-didehydroergoline-8-carboxamide (323 Da, Ligand)
- 5-ht2b receptor bound to lsd (Complex from Homo sapiens)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7srr
Q-score: 0.564
Cao C, Barros-Alvarez X, Zhang S, Kim K, Damgen MA, Panova O, Suomivuori CM, Fay JF, Zhong X, Krumm BE, Gumpper RH, Seven AB, Robertson MJ, Krogan NJ, Huttenhain R, Nichols DE, Dror RO, Skiniotis G, Roth BL
Neuron (2022) 110 pp. 3154- [ DOI: doi:10.1016/j.neuron.2022.08.006 Pubmed: 36087581 ]
- Single-chain variable fragment 16 (scfv16) (30 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- 5-ht2b receptor bound to lsd in complex with heterotrimeric mini-gq protein and single-chain variable fragment (scfv16) (Complex from Homo sapiens)
- (8alpha)-n,n-diethyl-6-methyl-9,10-didehydroergoline-8-carboxamide (323 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- G protein subunit q (gi2-mini-gq chimera) (28 kDa, Protein from Homo sapiens)
- 5-hydroxytryptamine receptor 2b (41 kDa, Protein from Homo sapiens)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7srs
Q-score: 0.523
Cao C, Barros-Alvarez X, Zhang S, Kim K, Damgen MA, Panova O, Suomivuori CM, Fay JF, Zhong X, Krumm BE, Gumpper RH, Seven AB, Robertson MJ, Krogan NJ, Huttenhain R, Nichols DE, Dror RO, Skiniotis G, Roth BL
Neuron (2022) 110 pp. 3154- [ DOI: doi:10.1016/j.neuron.2022.08.006 Pubmed: 36087581 ]
- Anti-5ht2br fab heavy chain (23 kDa, Protein from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Anti-5ht2br fab light chain (22 kDa, Protein from Mus musculus)
- (8alpha)-n,n-diethyl-6-methyl-9,10-didehydroergoline-8-carboxamide (323 Da, Ligand)
- Fab30 heavy chain (13 kDa, Protein from Mus musculus)
- Isoform 1b of beta-arrestin-1 (41 kDa, Protein from Homo sapiens)
- 5-hydroxytryptamine receptor 2b (37 kDa, Protein from Homo sapiens)
- Fab30 light chain (11 kDa, Protein from Mus musculus)
- 5-ht2b receptor bound to lsd in complex with beta-arrestin1 (Complex)
Org 2274179-0-bound Thyrotropin Receptor
Faust B, Billesbolle CB, Suomivuori CM, Singh I, Zhang K, Hoppe N, Pinto AFM, Diedrich JK, Muftuoglu Y, Szkudlinski MW, Saghatelian A, Dror RO, Cheng Y, Manglik A
Nature (2022) 609 pp. 846-853 [ Pubmed: 35940205 DOI: doi:10.1038/s41586-022-05159-1 ]