Combined 3D structure of the isolated yeast NPC
Akey CW, Singh D, Ouch C, Echeverria I, Nudelman I, Varberg JM, Yu Z, Fang F, Shi Y, Wang J, Salzberg D, Song K, Xu C, Gumbart JC, Suslov S, Unruh J, Jaspersen SL, Chait BT, Sali A, Fernandez-Martinez J, Ludtke SJ, Villa E, Rout MP
Cell (2022) 185 pp. 361-378.e25 [ DOI: doi:10.1016/j.cell.2021.12.015 Pubmed: 34982960 ]
- Nsp1 complex connections to fg domains (Complex from Saccharomyces cerevisiae)
- Inner ring of spokes from npc (9 MDa, Complex from Saccharomyces cerevisiae)
- Double nuclear outer ring of nup84 complex pairs with nup188-192 (Complex from Saccharomyces cerevisiae)
- Isolated yeast npc (Complex)
- Central transporter (Complex from Saccharomyces cerevisiae)
- Pom152 ring (Complex from Saccharomyces cerevisiae)
Low resolution, multi-part 3D structure of the isolated yeast NPC
Akey CW, Singh D, Ouch C, Echeverria I, Nudelman I, Varberg JM, Yu Z, Fang F, Shi Y, Wang J, Salzberg D, Song K, Xu C, Gumbart JC, Suslov S, Unruh J, Jaspersen SL, Chait BT, Sali A, Fernandez-Martinez J, Ludtke SJ, Villa E, Rout MP
Cell (2021) [ DOI: doi:10.1016/j.cell.2021.12.015 Pubmed: 34982960 ]
- Double nuclear outer ring (Complex from Saccharomyces cerevisiae)
- 3d map of yeast npc (52 MDa, Complex)
- Nup82 complex hook ring (Complex from Saccharomyces cerevisiae)
- Central transporter (Complex from Saccharomyces cerevisiae)
- Inner spoke ring of yeast npc (Complex from Saccharomyces cerevisiae)
Double nuclear outer ring from the isolated yeast NPC
Resolution: 11.6 Å
EM Method: Single-particle
Fitted PDBs: 7n84
Q-score: 0.063
Akey CW, Singh D, Ouch C, Echeverria I, Nudelman I, Varberg JM, Yu Z, Fang F, Shi Y, Wang J, Salzberg D, Song K, Xu C, Gumbart JC, Suslov S, Unruh J, Jaspersen SL, Chait BT, Sali A, Fernandez-Martinez J, Ludtke SJ, Villa E, Rout MP
Cell (2022) 185 pp. 361-378.e25 [ DOI: doi:10.1016/j.cell.2021.12.015 Pubmed: 34982960 ]
- Nucleoporin seh1 (39 kDa, Protein from Saccharomyces cerevisiae)
- Unknown (5 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin nup188 (188 kDa, Protein from Saccharomyces cerevisiae)
- Protein transport protein sec13 (33 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin 145c (81 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin nup120 (120 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin nup85 (84 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin nup84 (83 kDa, Protein from Saccharomyces cerevisiae)
- Yeast double outer ring (10 MDa, Complex from Saccharomyces cerevisiae)
- Nucleoporin nup133 (133 kDa, Protein from Saccharomyces cerevisiae)
Inner ring spoke from the isolated yeast NPC
Resolution: 7.6 Å
EM Method: Single-particle
Fitted PDBs: 7n85
Q-score: 0.113
Akey CW, Singh D, Ouch C, Echeverria I, Nudelman I, Varberg JM, Yu Z, Fang F, Shi Y, Wang J, Salzberg D, Song K, Xu C, Gumbart JC, Suslov S, Unruh J, Jaspersen SL, Chait BT, Sali A, Fernandez-Martinez J, Ludtke SJ, Villa E, Rout MP
Cell (2022) 185 pp. 361-378.e25 [ DOI: doi:10.1016/j.cell.2021.12.015 Pubmed: 34982960 ]
- Spoke from yeast inner ring (16 MDa, Complex from Saccharomyces cerevisiae)
- Nucleoporin nup57 (57 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin nup192 (191 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin nup188 (188 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin nup53 (52 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin nsp1 (86 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin nup170 (169 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin nic96 (96 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin nup157 (156 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin nup49/nsp49 (49 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin asm4 (58 kDa, Protein from Saccharomyces cerevisiae)
- Unknown connectors (3 kDa, Protein from Saccharomyces cerevisiae)
Structure of the in situ yeast NPC
Resolution: 37.0 Å
EM Method: Subtomogram averaging
Fitted PDBs: 7n9f
Q-score: 0.043
Akey CW, Singh D, Ouch C, Echeverria I, Nudelman I, Varberg JM, Yu Z, Fang F, Shi Y, Wang J, Salzberg D, Song K, Xu C, Gumbart JC, Suslov S, Unruh J, Jaspersen SL, Chait BT, Sali A, Fernandez-Martinez J, Ludtke SJ, Villa E, Rout MP
Cell (2022) 185 pp. 361-378.e25 [ DOI: doi:10.1016/j.cell.2021.12.015 Pubmed: 34982960 ]
- Nucleoporin nup57 (57 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin nup192 (191 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin nup53 (52 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin 145c (81 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin nup85 (84 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin nic96 (96 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin nsp1 (86 kDa, Protein from Saccharomyces cerevisiae)
- Yeast npc (52 MDa, Cellular component from Saccharomyces cerevisiae)
- Nucleoporin nup170 (169 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin nup133 (133 kDa, Protein from Saccharomyces cerevisiae)
- Dynein light chain 1, cytoplasmic (10 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin nup188 (188 kDa, Protein from Saccharomyces cerevisiae)
- Protein transport protein sec13 (33 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin nup84 (83 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin nup157 (156 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin nup49/nsp49 (49 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin seh1 (39 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin nup120 (120 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin nup159 (159 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin nup82 (82 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin asm4 (58 kDa, Protein from Saccharomyces cerevisiae)
- Orphans bound to nup192 ntd (3 kDa, Protein from Saccharomyces cerevisiae)