RecBCD-DNA in complex with the phage protein Abc2
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8b1t
Q-score: 0.463
Wilkinson M, Wilkinson OJ, Feyerherm C, Fletcher EE, Wigley DB, Dillingham MS
eLife (2022) 11 [ DOI: doi:10.7554/eLife.83409 Pubmed: 36533901 ]
- Dna (70-mer) (Complex from synthetic construct)
- Dna (70-mer) (21 kDa, DNA from synthetic construct)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Recbcd enzyme subunit recc (128 kDa, Protein from Escherichia coli)
- Recbcd-abc2-dna complex (Complex)
- Recbcd enzyme subunit recd (66 kDa, Protein from Escherichia coli)
- Recbcd enzyme subunit recb, recc and recd (Complex from Escherichia coli)
- Recbcd enzyme subunit recb (134 kDa, Protein from Escherichia coli)
- Anti-recbcd protein 2 (11 kDa, Protein from Salmonella phage P22)
- Magnesium ion (24 Da, Ligand)
- Anti-recbcd protein 2 (Complex from Salmonella phage P22)
RecBCD-DNA in complex with the phage protein Abc2 and host PpiB
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8b1u
Q-score: 0.387
Wilkinson M, Wilkinson OJ, Feyerherm C, Fletcher EE, Wigley DB, Dillingham MS
eLife (2022) 11 [ DOI: doi:10.7554/eLife.83409 Pubmed: 36533901 ]
- Recbcd enzyme subunits recb, recc and recd (Complex from Escherichia coli)
- Dna (70-mer) (Complex from synthetic construct)
- Dna (70-mer) (21 kDa, DNA from synthetic construct)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Recbcd enzyme subunit recc (128 kDa, Protein from Escherichia coli)
- Recbcd enzyme subunit recd (66 kDa, Protein from Escherichia coli)
- Recbcd-abc2-ppib-dna complex (Complex)
- Recbcd enzyme subunit recb (134 kDa, Protein from Escherichia coli)
- Anti-recbcd protein 2 (11 kDa, Protein from Salmonella phage P22)
- Magnesium ion (24 Da, Ligand)
- Anti-recbcd protein 2 (Complex from Salmonella phage P22)
Cryo-EM structure of the RecBCD Chi recognised complex
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6sjb
Q-score: 0.388
Cheng K, Wilkinson M, Chaban Y, Wigley DB
Nat Struct Mol Biol (2020) 27 pp. 71-77 [ DOI: doi:10.1038/s41594-019-0355-2 Pubmed: 31907455 ]
- Recbcd enzyme subunit recb (134 kDa, Protein from Escherichia coli)
- Dna fork substrate (26 kDa, DNA from synthetic construct)
- Recbcd bound to a forked chi-containing dna substrate (355 kDa, Complex)
- Dna substrate (Complex from synthetic construct)
- Recbcd enzyme subunit recc (128 kDa, Protein from Escherichia coli)
- Recbcd enzyme subunit recd (66 kDa, Protein from Escherichia coli)
- Recbcd (Complex from Escherichia coli)
Cryo-EM structure of the RecBCD Chi partially-recognised complex
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6sje
Q-score: 0.315
Cheng K, Wilkinson M, Chaban Y, Wigley DB
Nat Struct Mol Biol (2020) 27 pp. 71-77 [ DOI: doi:10.1038/s41594-019-0355-2 Pubmed: 31907455 ]
- Recbcd enzyme subunit recb (134 kDa, Protein from Escherichia coli)
- Dna fork substrate (26 kDa, DNA from synthetic construct)
- Recbcd bound to a forked chi-containing dna substrate (355 kDa, Complex)
- Recbcd enzyme subunit recc (128 kDa, Protein from Escherichia coli)
- Recbcd enzyme subunit recd (66 kDa, Protein from Escherichia coli)
- Forked chi-containing dna substrate (Complex from synthetic construct)
- Recbcd (Complex from Escherichia coli)
Cryo-EM structure of the RecBCD Chi unrecognised complex
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6sjf
Q-score: 0.353
Cheng K, Wilkinson M, Chaban Y, Wigley DB
Nat Struct Mol Biol (2020) 27 pp. 71-77 [ DOI: doi:10.1038/s41594-019-0355-2 Pubmed: 31907455 ]
- Recbcd enzyme subunit recb (134 kDa, Protein from Escherichia coli)
- Dna (Complex from synthetic construct)
- Recbcd bound to a forked chi-containing dna substrate (355 kDa, Complex)
- Forked dna substrate (26 kDa, DNA from synthetic construct)
- Recbcd enzyme subunit recc (128 kDa, Protein from Escherichia coli)
- Recbcd enzyme subunit recd (66 kDa, Protein from Escherichia coli)
- Recbcd (Complex from Escherichia coli)
Cryo-EM structure of the RecBCD no Chi negative control complex
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6sjg
Q-score: 0.306
Cheng K, Wilkinson M, Chaban Y, Wigley DB
Nat Struct Mol Biol (2020) 27 pp. 71-77 [ DOI: doi:10.1038/s41594-019-0355-2 Pubmed: 31907455 ]
- Recbcd enzyme subunit recb (134 kDa, Protein from Escherichia coli)
- Recbcd bound to a forked dna substrate that does not contain chi (355 kDa, Complex)
- Dna (Complex from synthetic construct)
- Forked dna substrate (26 kDa, DNA from synthetic construct)
- Recbcd enzyme subunit recc (128 kDa, Protein from Escherichia coli)
- Recbcd enzyme subunit recd (66 kDa, Protein from Escherichia coli)
- Recbcd (Complex from Escherichia coli)
Cryo-EM structure of the RecBCD in complex with Chi-minus2 substrate
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6t2u
Q-score: 0.329
Cheng K, Wilkinson M, Chaban Y, Wigley DB
Nat Struct Mol Biol (2020) 27 pp. 71-77 [ DOI: doi:10.1038/s41594-019-0355-2 Pubmed: 31907455 ]
- Recbcd bound to a forked dna substrate that contain chi-minus2 (355 kDa, Complex)
- Recbcd enzyme subunit recc (128 kDa, Protein from Escherichia coli)
- Chi-minus2 (Complex from synthetic construct)
- Recbcd enzyme subunit recd (66 kDa, Protein from Escherichia coli)
- Dna (chi-minus2) (25 kDa, DNA from synthetic construct)
- Recbcd (Complex from Escherichia coli)
- Recbcd enzyme subunit recb (134 kDa, Protein from Escherichia coli)
Cryo-EM structure of the RecBCD in complex with Chi-plus2 substrate
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6t2v
Q-score: 0.28
Cheng K, Wilkinson M, Chaban Y, Wigley DB
Nat Struct Mol Biol (2020) 27 pp. 71-77 [ DOI: doi:10.1038/s41594-019-0355-2 Pubmed: 31907455 ]
- Recbcd enzyme subunit recb (134 kDa, Protein from Escherichia coli)
- Dna (chi-plus2) (26 kDa, DNA from synthetic construct)
- Recbcd enzyme subunit recc (128 kDa, Protein from Escherichia coli)
- Recbcd enzyme subunit recd (66 kDa, Protein from Escherichia coli)
- Recbdcd (Complex from Escherichia coli)
- Recbcd bound to a forked dna substrate that contain chi-plus2 (355 kDa, Complex)
- Chi-plus2 (Complex from synthetic construct)
High resolution INO80 core complex
Resolution: 4.06 Å
EM Method: Single-particle
Fitted PDBs: 5oaf
Q-score: 0.315
Aramayo RJ, Willhoft O, Ayala R, Bythell-Douglas R, Wigley DB, Zhang X
Nat Struct Mol Biol (2018) 25 pp. 37-44 [ Pubmed: 29323271 DOI: doi:10.1038/s41594-017-0003-7 ]
- Ruvb-like 1 (50 kDa, Protein from Homo sapiens)
- Human ino80 core complex (800 kDa, Complex from Homo sapiens)
- Ruvb-like 2 (51 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Cryo-EM structure of Lambda Phage protein GamS bound to RecBCD
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 5mbv
Q-score: 0.334
Wilkinson M, Troman L, Wan Nur Ismah WA, Chaban Y, Avison MB, Dillingham MS, Wigley DB
eLife (2016) 5 [ DOI: doi:10.7554/eLife.22963 Pubmed: 28009252 ]
- Recbcd complex bound to inhibitory protein lambda gams (350 kDa, Complex)
- Recbcd helicase/nuclease complex (330 kDa, Complex from Escherichia coli)
- Recbcd enzyme subunit recc (128 kDa, Protein from Escherichia coli)
- Lambda gams (20 kDa, Complex from Enterobacteria phage lambda)
- Host-nuclease inhibitor protein gam (11 kDa, Protein from Enterobacteria phage lambda)
- Recbcd enzyme subunit recd (67 kDa, Protein from Escherichia coli)
- Recbcd enzyme subunit recb (134 kDa, Protein from Escherichia coli)