Gq bound FZD1 in ligand-free state
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8j9n
Q-score: 0.386
Zhang Z, Lin X, Wei L, Wu Y, Xu L, Wu L, Wei X, Zhao S, Zhu X, Xu F
Cell Discov (2024) 10 pp. 3-3 [ DOI: doi:10.1038/s41421-023-00627-y Pubmed: 38182578 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Frizzled-1 (64 kDa, Protein from Homo sapiens)
- Nanobody nb35 (16 kDa, Protein from Vicugna pacos)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,guanine nucleotide-binding protein g(q) subunit alpha (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Fzd1-gq complex in the ligand-free state (Complex from Homo sapiens)
Cryo-EM structure of inactive FZD1
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8j9o
Q-score: 0.347
Zhang Z, Lin X, Wei L, Wu Y, Xu L, Wu L, Wei X, Zhao S, Zhu X, Xu F
Cell Discov (2024) 10 pp. 3-3 [ DOI: doi:10.1038/s41421-023-00627-y Pubmed: 38182578 ]
- Anti-bril fab light chain (24 kDa, Protein from Mus)
- Anti-bril fab heavy chain (28 kDa, Protein from Mus)
- Frizzled-1,soluble cytochrome b562 (75 kDa, Protein from Homo sapiens)
- Anti-fab nanobody (13 kDa, Protein from Vicugna pacos)
- Frizzled-1 with icl3-bril complex with anti-bril fab (Complex from Homo sapiens)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8jh7
Q-score: 0.354
Zhang Z, Lin X, Wei L, Wu Y, Xu L, Wu L, Wei X, Zhao S, Zhu X, Xu F
Cell Discov (2024) 10 pp. 3-3 [ DOI: doi:10.1038/s41421-023-00627-y Pubmed: 38182578 ]
- Anti-bril fab heavy chain (25 kDa, Protein from Homo sapiens)
- Anti-fab nanobody (14 kDa, Protein from synthetic construct)
- Complex of human fzd6 in inactivate state with accessory proteins. (Complex from Homo sapiens)
- Frizzled-6,soluble cytochrome b562 (73 kDa, Protein from Homo sapiens)
- Anti-bril fab light chain (23 kDa, Protein from Homo sapiens)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8jhb
Q-score: 0.334
Zhang Z, Lin X, Wei L, Wu Y, Xu L, Wu L, Wei X, Zhao S, Zhu X, Xu F
Cell Discov (2024) 10 pp. 3-3 [ DOI: doi:10.1038/s41421-023-00627-y Pubmed: 38182578 ]
- Nb35 (14 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms xlas (31 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (8 kDa, Protein from Homo sapiens)
- Frizzled-6 (61 kDa, Protein from Homo sapiens)
- Fzd6-gs complex with nb35. (Complex from Homo sapiens)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8jhc
Q-score: 0.387
Zhang Z, Lin X, Wei L, Wu Y, Xu L, Wu L, Wei X, Zhao S, Zhu X, Xu F
Cell Discov (2024) 10 pp. 3-3 [ DOI: doi:10.1038/s41421-023-00627-y Pubmed: 38182578 ]
- Frizzled-3,soluble cytochrome b562 (74 kDa, Protein from Homo sapiens)
- Anti-bril fab heavy chain (25 kDa, Protein from Homo sapiens)
- Complex of fzd3 with anti-bril fab and anti-fab nanobody (Complex from Homo sapiens)
- Anti-fab nanobody (14 kDa, Protein from synthetic construct)
- Anti-bril fab light chain (23 kDa, Protein from Homo sapiens)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8jhi
Q-score: 0.423
Zhang Z, Lin X, Wei L, Wu Y, Xu L, Wu L, Wei X, Zhao S, Zhu X, Xu F
Cell Discov (2024) 10 pp. 3-3 [ DOI: doi:10.1038/s41421-023-00627-y Pubmed: 38182578 ]
- Complex of fzd3-gs heterotrimer with nb35. (Complex from Homo sapiens)
- Nb35 (13 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms xlas (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Frizzled-3 (60 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
Orphan GPR20 in complex with Fab046
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 8hs2
Q-score: 0.479
Lin X, Jiang S, Wu Y, Wei X, Han GW, Wu L, Liu J, Chen B, Zhang Z, Zhao S, Cherezov V, Xu F
Cell Discov (2023) 9 pp. 23-23 [ DOI: doi:10.1038/s41421-023-00520-8 Pubmed: 36849514 ]
- Soluble cytochrome b562,g-protein coupled receptor 20 (50 kDa, Protein from Homo sapiens)
- The complex of gpr20 with fab046 (Complex from Homo sapiens)
- Heavy chain of fab046 (26 kDa, Protein from Rattus)
- Light chain of fab046 (25 kDa, Protein from Rattus)
Gi bound orphan GPR20 in ligand-free state
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 8hs3
Q-score: 0.483
Lin X, Jiang S, Wu Y, Wei X, Han GW, Wu L, Liu J, Chen B, Zhang Z, Zhao S, Cherezov V, Xu F
Cell Discov (2023) 9 pp. 23-23 [ DOI: doi:10.1038/s41421-023-00520-8 Pubmed: 36849514 ]
- Scfv16 (31 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Orphan gpr20 complex with gi trimer in the absence of a ligand (Complex from Homo sapiens)
- Ggama (8 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,g-protein coupled receptor 20 (56 kDa, Protein from Homo sapiens)
Gi bound Orphan GPR20 complex with Fab046 in ligand-free state
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 8hsc
Q-score: 0.467
Lin X, Jiang S, Wu Y, Wei X, Han GW, Wu L, Liu J, Chen B, Zhang Z, Zhao S, Cherezov V, Xu F
Cell Discov (2023) 9 pp. 23-23 [ DOI: doi:10.1038/s41421-023-00520-8 Pubmed: 36849514 ]
- The complex of gi bound orphan gpr20 with fab046 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Scfv16 (31 kDa, Protein from Vicugna pacos)
- Light chain of fab046 (25 kDa, Protein from Rattus)
- Soluble cytochrome b562,g-protein coupled receptor 20 (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Heavy chain of fab046 (25 kDa, Protein from Rattus)