Resolution: 3.53 Å
EM Method: Single-particle
Fitted PDBs: 8dhb
Q-score: 0.297
Jansen RM, Peruzzo R, Fromm SA, Yokom AL, Zoncu R, Hurley JH
Sci Adv (2022) 8 pp. eadd2926-eadd2926 [ DOI: doi:10.1126/sciadv.add2926 Pubmed: 36103527 ]
- [(2~{r},3~{s},4~{r},5~{r})-5-[2,6-bis(oxidanylidene)-3~{h}-purin-9-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl phosphono hydrogen phosphate (444 Da, Ligand)
- Ras-related gtp-binding protein c (44 kDa, Protein from Homo sapiens)
- Glutathione s-transferase class-mu 26 kda isozyme,sodium-coupled neutral amino acid transporter 9 chimera (41 kDa, Protein from Homo sapiens)
- Flcn:slc-fnip2 (Complex from Homo sapiens)
- Ras-related gtp-binding protein a (39 kDa, Protein from Homo sapiens)
- Folliculin-interacting protein 2 (149 kDa, Protein from Homo sapiens)
- Beryllium trifluoride ion (66 Da, Ligand)
- Ragulator complex protein lamtor1 (18 kDa, Protein from Homo sapiens)
- Ragulator complex protein lamtor2 (13 kDa, Protein from Homo sapiens)
- Cryo-em structure of the active flcn gap compex (Complex from Homo sapiens)
- Ragulator complex protein lamtor3 (13 kDa, Protein from Homo sapiens)
- Ragulator complex protein lamtor4 (10 kDa, Protein from Homo sapiens)
- Ragulator complex protein lamtor5 (18 kDa, Protein from Homo sapiens)
- Folliculin (69 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Rag-ragulator (Complex from Homo sapiens)
Cryo-EM Structure of the Lysosomal Folliculin Complex (FLCN-FNIP2-RagA-RagC-Ragulator)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6nzd
Q-score: 0.326
Lawrence RE, Fromm SA, Fu Y, Yokom AL, Kim DJ, Thelen AM, Young LN, Lim CY, Samelson AJ, Hurley JH, Zoncu R
Science (2019) 366 pp. 971-977 [ Pubmed: 31672913 DOI: doi:10.1126/science.aax0364 ]
- Flcn-fnip2-raga-ragc-ragulator complex (340 kDa, Complex from Homo sapiens)
- Ras-related gtp-binding protein c (44 kDa, Protein from Homo sapiens)
- Ras-related gtp-binding protein a (36 kDa, Protein from Homo sapiens)
- Ragulator complex protein lamtor1 (18 kDa, Protein from Homo sapiens)
- 9-{5-o-[(s)-hydroxy{[(r)-hydroxy(thiophosphonooxy)phosphoryl]oxy}phosphoryl]-alpha-l-arabinofuranosyl}-3,9-dihydro-1h-purine-2,6-dione (540 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Ragulator complex protein lamtor2 (13 kDa, Protein from Homo sapiens)
- Hepatitis b virus x interacting protein (18 kDa, Protein from Homo sapiens)
- Folliculin (69 kDa, Protein from Homo sapiens)
- Ragulator complex protein lamtor4 (10 kDa, Protein from Homo sapiens)
- Folliculin-interacting protein 2 (122 kDa, Protein from Homo sapiens)
- Ragulator complex protein lamtor3 (13 kDa, Protein from Homo sapiens)
Cryo-EM Map of the active Ragulator-RagA-RagC Complex
Lawrence RE, Fromm SA, Fu Y, Yokom AL, Kim DJ, Thelen AM, Young LN, Lim CY, Samelson AJ, Hurley JH, Zoncu R
Science (2019) 366 pp. 971-977 [ Pubmed: 31672913 DOI: doi:10.1126/science.aax0364 ]
- Active raga-ragc-ragulator complex (150 kDa, Complex from Homo sapiens)
Inactive State of Manduca sexta soluble guanylate cyclase
Resolution: 5.1 Å
EM Method: Single-particle
Fitted PDBs: 6pas
Q-score: 0.284
Horst BG, Yokom AL, Rosenberg DJ, Morris KL, Hammel M, Hurley JH, Marletta MA
eLife (2019) 8 [ DOI: doi:10.7554/eLife.50634 Pubmed: 31566566 ]
- Soluble guanylyl cyclase beta-1 subunit (68 kDa, Protein from Manduca sexta)
- Soluble guanylyl cyclase alpha-1 subunit (78 kDa, Protein from Manduca sexta)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Inactive state of heterodimeric ms sgc (Complex from Manduca sexta)
Active State of Manduca sexta soluble Guanylate Cyclase
Resolution: 5.8 Å
EM Method: Single-particle
Fitted PDBs: 6pat
Q-score: 0.323
Horst BG, Yokom AL, Rosenberg DJ, Morris KL, Hammel M, Hurley JH, Marletta MA
eLife (2019) 8 [ DOI: doi:10.7554/eLife.50634 Pubmed: 31566566 ]
- Soluble guanylyl cyclase beta-1 subunit (68 kDa, Protein from Manduca sexta)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Soluble guanylyl cyclase alpha-1 subunit (78 kDa, Protein from Manduca sexta)
- Active state of heterodimeric ms sgc (Complex from Manduca sexta)
S. cerevisiae Hsp104:casein complex, Middle Domain Conformation
Resolution: 6.7 Å
EM Method: Single-particle
Fitted PDBs: 5vy9
Q-score: 0.161
Gates SN, Yokom AL, Lin J, Jackrel ME, Rizo AN, Kendsersky NM, Buell CE, Sweeny EA, Mack KL, Chuang E, Torrente MP, Su M, Shorter J, Southworth DR
Science (2017) 357 pp. 273-279 [ Pubmed: 28619716 DOI: doi:10.1126/science.aan1052 ]
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Hsp104 (Complex)
- Heat shock protein 104 (102 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- S. cerevisiae hsp104:casein complex, middle domain conformation (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Casein (Complex from Bos taurus)
- Alpha-s1-casein (2 kDa, Protein from Bos taurus)
Closed State CryoEM Reconstruction of Hsp104:ATPyS and FITC casein
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 5vjh
Q-score: 0.328
Gates SN, Yokom AL, Lin J, Jackrel ME, Rizo AN, Kendsersky NM, Buell CE, Sweeny EA, Mack KL, Chuang E, Torrente MP, Su M, Shorter J, Southworth DR
Science (2017) 357 pp. 273-279 [ Pubmed: 28619716 DOI: doi:10.1126/science.aan1052 ]
- Fitc casein (2 kDa, Protein from bovine)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Hsp104 atpys and fitc casein in closed state (Complex from Saccharomyces cerevisiae)
- Heat shock protein 104 (102 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
S. cerevisiae Hsp104-ADP complex
Resolution: 5.6 Å
EM Method: Single-particle
Fitted PDBs: 5vy8
Q-score: 0.187
Gates SN, Yokom AL, Lin J, Jackrel ME, Rizo AN, Kendsersky NM, Buell CE, Sweeny EA, Mack KL, Chuang E, Torrente MP, Su M, Shorter J, Southworth DR
Science (2017) 357 pp. 273-279 [ DOI: doi:10.1126/science.aan1052 Pubmed: 28619716 ]
S. cerevisiae Hsp104:casein complex, Extended Conformation
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 5vya
Q-score: 0.313
Gates SN, Yokom AL, Lin J, Jackrel ME, Rizo AN, Kendsersky NM, Buell CE, Sweeny EA, Mack KL, Chuang E, Torrente MP, Su M, Shorter J, Southworth DR
Science (2017) 357 pp. 273-279 [ DOI: doi:10.1126/science.aan1052 Pubmed: 28619716 ]
- S. cerevisiae hsp104:casein complex, extended conformation (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Heat shock protein 104 (102 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Alpha-s1-casein (2 kDa, Protein from Bovine)