Cryo-EM structure of the transcription activation complex NtcA-TAC
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8h40
Q-score: 0.353
Han SJ, Jiang YL, You LL, Shen LQ, Wu X, Yang F, Cui N, Kong WW, Sun H, Zhou K, Meng HC, Chen ZP, Chen Y, Zhang Y, Zhou CZ
Nat Struct Mol Biol (2024) 31 pp. 293-299 [ Pubmed: 38177666 DOI: doi:10.1038/s41594-023-01149-7 ]
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Anabaena)
- Transcription activation complex with the global regulator ntca (580 kDa, Complex from Anabaena)
- Dna-directed rna polymerase subunit gamma (70 kDa, Protein from Anabaena)
- Dna (125-mer) (38 kDa, DNA from Anabaena)
- Ntca (24 kDa, Protein from Anabaena)
- Rna polymerase sigma factor siga (45 kDa, Protein from Anabaena)
- Dna-directed rna polymerase subunit omega (9 kDa, Protein from Anabaena)
- Dna (125-mer) (38 kDa, DNA from Anabaena)
- Dna-directed rna polymerase subunit beta' (147 kDa, Protein from Anabaena)
- Dna-directed rna polymerase subunit beta (126 kDa, Protein from Anabaena)
Cryo-EM structure of Synechocystis sp. PCC 6803 RPitc
Resolution: 3.13 Å
EM Method: Single-particle
Fitted PDBs: 8gzg
Q-score: 0.488
PNAS (2023) [ ]
- Nontemplate strand dna (20 kDa, DNA from synthetic construct)
- Rna (5'-r(*up*cp*gp*a)-3') (1 kDa, RNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Template strand dna (20 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit gamma (71 kDa, Protein from Synechocystis sp. PCC 6803)
- Dna-directed rna polymerase subunit beta' (145 kDa, Protein from Synechocystis sp. PCC 6803)
- The complex of synechocystis sp. pcc 6803 rpitc (500 kDa, Complex from Synechocystis sp. PCC 6803)
- Dna-directed rna polymerase subunit omega (8 kDa, Protein from Synechocystis sp. PCC 6803)
- Dna-directed rna polymerase subunit beta (123 kDa, Protein from Synechocystis sp. PCC 6803)
- Rna polymerase sigma factor siga (50 kDa, Protein from Synechocystis sp. PCC 6803)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Synechocystis sp. PCC 6803)
The cryo-EM structure of A. thaliana Pol IV-RDR2 backtracked complex
Resolution: 3.16 Å
EM Method: Single-particle
Fitted PDBs: 7eu0
Q-score: 0.487
Huang K, Wu XX, Fang CL, Xu ZG, Zhang HW, Gao J, Zhou CM, You LL, Gu ZX, Mu WH, Feng Y, Wang JW, Zhang Y
Science (2021) 374 pp. 1579-1586 [ Pubmed: 34941388 DOI: doi:10.1126/science.abj9184 ]
- Rna-dependent rna polymerase 2 (129 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 6a (16 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii and iv subunit 5a (24 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 3 (35 kDa, Protein from Arabidopsis thaliana)
- A. thaliana pol iv-rdr2 backtracked complex (550 kDa, Complex from Arabidopsis thaliana)
- Dna-directed rna polymerases iv and v subunit 2 (132 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 8b (16 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 12 (5 kDa, Protein from Arabidopsis thaliana)
- Dna (33-mer) (9 kDa, DNA from synthetic construct)
- Dna-directed rna polymerases ii, iv and v subunit 9a (13 kDa, Protein from Arabidopsis thaliana)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerases ii, iv and v subunit 10 (8 kDa, Protein from Arabidopsis thaliana)
- Dna (5'-d(*cp*tp*gp*tp*gp*cp*tp*tp*ap*tp*cp*gp*gp*tp*ap*gp*ap*gp*tp*g)-3') (6 kDa, DNA from synthetic construct)
- Rna (5'-r(*cp*cp*gp*a)-3') (1 kDa, RNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Rna (39-mer) (12 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase iv subunit 1 (169 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 11 (13 kDa, Protein from Arabidopsis thaliana)
The cryo-EM structure of A. thaliana Pol IV-RDR2 holoenzyme
Resolution: 3.86 Å
EM Method: Single-particle
Fitted PDBs: 7eu1
Q-score: 0.434
Huang K, Wu XX, Fang CL, Xu ZG, Zhang HW, Gao J, Zhou CM, You LL, Gu ZX, Mu WH, Feng Y, Wang JW, Zhang Y
Science (2021) 374 pp. 1579-1586 [ Pubmed: 34941388 DOI: doi:10.1126/science.abj9184 ]
- Dna-directed rna polymerases ii, iv and v subunit 9a (13 kDa, Protein from Arabidopsis thaliana)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerases ii, iv and v subunit 11 (13 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 8b (16 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 10 (8 kDa, Protein from Arabidopsis thaliana)
- Rna-dependent rna polymerase 2 (129 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 6a (16 kDa, Protein from Arabidopsis thaliana)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerases ii, iv and v subunit 3 (35 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerase iv subunit 1 (169 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 12 (5 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases iv and v subunit 2 (132 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii and iv subunit 5a (24 kDa, Protein from Arabidopsis thaliana)
- A. thaliana pol iv-rdr2 holoenzyme (Complex from Arabidopsis thaliana)