Electron Microscopy Data Bank

EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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primary_citation_author:"Yu L" AND microscope_name:"FEI TALOS ARCTICA" AND author_orcid:"0000-0003-0190-6528"

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EMD-24424

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Cryo-EM structure of Thermus thermophilus reiterative transcription complex with 11nt oligo-G RNA

Release date: Feb. 2, 2022
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7rdq
Q-score: 0.516

Liu Y, Yu L, Pukhrambam C, Winkelman JT, Firlar E, Kaelber JT, Zhang Y, Nickels BE, Ebright RH

PNAS (2022) 119 [ DOI: doi:10.1073/pnas.2115746119 Pubmed: 35082149 ]

Sample:

Rna (5'-r(p*gp*gp*gp*gp*gp*gp*gp*gp*gp*gp*g)-3') (3 kDa, RNA from synthetic construct)
Dna (31-mer) template strand (10 kDa, DNA from synthetic construct)
Dna-directed rna polymerase subunit beta' (170 kDa, Protein from Thermus thermophilus HB8)
Dna-directed rna polymerase subunit omega (11 kDa, Protein from Thermus thermophilus HB8)
Dna-directed rna polymerase subunit alpha (35 kDa, Protein from Thermus thermophilus HB8)
Rna polymerase sigma factor siga (48 kDa, Protein from Thermus thermophilus HB8)
Reiterative transcription initiation complex with 11nt oligo-g rna (425 kDa, Complex from Thermus thermophilus HB8)
Magnesium ion (24 Da, Ligand)
Zinc ion (65 Da, Ligand)
Dna-directed rna polymerase subunit beta (125 kDa, Protein from Thermus thermophilus HB8)
Dna (31-mer) nontemplate strand (10 kDa, DNA from synthetic construct)

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