Cryo-EM structure of the EfPiwi (N959K)-piRNA-target ternary complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7yfq
Q-score: 0.435
Li Z, Li Z, Zhang Y, Zhou L, Xu Q, Li L, Zeng L, Xue J, Niu H, Zhong J, Yu Q, Li D, Gui M, Huang Y, Tu S, Zhang Z, Song CQ, Wu J, Shen EZ
Nat Struct Mol Biol (2024) [ Pubmed: 38658622 DOI: doi:10.1038/s41594-024-01287-6 ]
- Pirna (7 kDa, RNA from Homo sapiens)
- Piwi (92 kDa, Protein from Ephydatia fluviatilis)
- Rna (5'-r(*up*cp*cp*ap*up*gp*up*up*gp*ap*up*gp*gp*up*ap*a)-3') (5 kDa, RNA from Homo sapiens)
- Rna (Complex)
- Efpiwi(n959k)-pirna-16nt target (Complex)
- Efpiwi(n959k) (Complex from Ephydatia fluviatilis)
- Magnesium ion (24 Da, Ligand)
Cryo-EM structure of Hili in complex with piRNA
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7yfx
Q-score: 0.425
Li Z, Li Z, Zhang Y, Zhou L, Xu Q, Li L, Zeng L, Xue J, Niu H, Zhong J, Yu Q, Li D, Gui M, Huang Y, Tu S, Zhang Z, Song CQ, Wu J, Shen EZ
Nat Struct Mol Biol (2024) [ Pubmed: 38658622 DOI: doi:10.1038/s41594-024-01287-6 ]
- Pirna (Complex)
- Magnesium ion (24 Da, Ligand)
- Pirna (8 kDa, RNA from Homo sapiens)
- Piwi-like protein 2 (114 kDa, Protein from Homo sapiens)
- Hili-pirna (Complex)
- Hili (Complex from Homo sapiens)
Cryo-EM structure of the Mili-piRNA- target ternary complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7yfy
Q-score: 0.406
Li Z, Li Z, Zhang Y, Zhou L, Xu Q, Li L, Zeng L, Xue J, Niu H, Zhong J, Yu Q, Li D, Gui M, Huang Y, Tu S, Zhang Z, Song CQ, Wu J, Shen EZ
Nat Struct Mol Biol (2024) [ DOI: doi:10.1038/s41594-024-01287-6 Pubmed: 38658622 ]
- Mili-pirna-15nt target (Complex)
- Rna (Complex)
- Rna (5'-r(p*cp*cp*ap*up*gp*up*up*gp*ap*up*gp*gp*up*ap*a)-3') (4 kDa, RNA from Homo sapiens)
- Pirna (8 kDa, RNA from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Mili (Complex from Mus musculus)
- Piwi-like protein 2 (91 kDa, Protein from Mus musculus)
Cryo-EM structure of the EfPiwi(N959K) in complex with piRNA
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7yg6
Q-score: 0.399
Li Z, Li Z, Zhang Y, Zhou L, Xu Q, Li L, Zeng L, Xue J, Niu H, Zhong J, Yu Q, Li D, Gui M, Huang Y, Tu S, Zhang Z, Song CQ, Wu J, Shen EZ
Nat Struct Mol Biol (2024) [ Pubmed: 38658622 DOI: doi:10.1038/s41594-024-01287-6 ]
- Pirna (7 kDa, RNA from Homo sapiens)
- Piwi (92 kDa, Protein from Ephydatia fluviatilis)
- Pirna (Complex)
- Efpiwi(n959k) (Complex from Ephydatia fluviatilis)
- Magnesium ion (24 Da, Ligand)
- Efpiwi(n959k)-pirna (Complex)
Cryo-EM structure of the Mili in complex with piRNA
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7ygn
Q-score: 0.5
Li Z, Li Z, Zhang Y, Zhou L, Xu Q, Li L, Zeng L, Xue J, Niu H, Zhong J, Yu Q, Li D, Gui M, Huang Y, Tu S, Zhang Z, Song CQ, Wu J, Shen EZ
Nat Struct Mol Biol (2024) [ Pubmed: 38658622 DOI: doi:10.1038/s41594-024-01287-6 ]
- Pirna (8 kDa, RNA from Homo sapiens)
- Mili-pirna (Complex)
- Mili (Complex from Mus musculus)
- Pirna (Complex)
- Magnesium ion (24 Da, Ligand)
- Piwi-like protein 2 (91 kDa, Protein from Mus musculus)