6-Deoxyerythronolide B synthase (DEBS) hybrid module (M3/1) in complex with antibody fragment 1B2
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7m7e
Q-score: 0.449
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ Pubmed: 34735239 DOI: doi:10.1126/science.abi8358 ]
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
- 6-deoxyerythronolide-b synthase erya2, modules 3 and 4,eryai,6-deoxyerythronolide-b synthase erya3, modules 5 and 6 chimera (187 kDa, Protein from Saccharopolyspora erythraea)
- Complex between debs m3/1te and antibody fragment 1b2 (430 kDa, Complex from Saccharopolyspora erythraea)
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)
6-Deoxyerythronolide B synthase (DEBS) module 1 in complex with antibody fragment 1B2: State 1
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7m7f
Q-score: 0.403
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ Pubmed: 34735239 DOI: doi:10.1126/science.abi8358 ]
- N~3~-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-n-(2-sulfanylethyl)-beta-alaninamide (358 Da, Ligand)
- Eryai,6-deoxyerythronolide-b synthase erya3, modules 5 and 6 chimera (188 kDa, Protein from Saccharopolyspora erythraea)
- Complex between debs (3)m1te and antibody fragment 1b2 (480 kDa, Complex from Saccharopolyspora erythraea)
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)
6-Deoxyerythronolide B synthase (DEBS) module 1 in complex with antibody fragment 1B2: State 2
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7m7g
Q-score: 0.181
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ Pubmed: 34735239 DOI: doi:10.1126/science.abi8358 ]
- Complex between debs (3)m1te and antibody fragment 1b2 (480 kDa, Complex from Saccharopolyspora erythraea)
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
- Eryai,6-deoxyerythronolide-b synthase erya3, modules 5 and 6 chimera (188 kDa, Protein from Saccharopolyspora erythraea)
6-Deoxyerythronolide B synthase (DEBS) module 1 in complex with antibody fragment 1B2: State 1'
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7m7h
Q-score: 0.19
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ Pubmed: 34735239 DOI: doi:10.1126/science.abi8358 ]
- Complex between debs (3)m1te and antibody fragment 1b2 (480 kDa, Complex from Saccharopolyspora erythraea)
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
- Eryai,6-deoxyerythronolide-b synthase erya3, modules 5 and 6 chimera (188 kDa, Protein from Saccharopolyspora erythraea)
6-Deoxyerythronolide B synthase (DEBS) module 1 in complex with antibody fragment 1B2 (TE-free)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7m7i
Q-score: 0.304
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ Pubmed: 34735239 DOI: doi:10.1126/science.abi8358 ]
- 1b2 (heavy chain) (25 kDa, Protein from Homo sapiens)
- N~3~-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-n-(2-sulfanylethyl)-beta-alaninamide (358 Da, Ligand)
- Eryai (167 kDa, Protein from Saccharopolyspora erythraea)
- 1b2 (light chain) (26 kDa, Protein from Saccharopolyspora erythraea)
- Complex between debs (3)m1(2) and antibody fragment 1b2 (440 kDa, Complex from Saccharopolyspora erythraea)
Resolution: 1.27 Å
EM Method: Single-particle
Fitted PDBs: 7rrp
Q-score: 0.908
Zhang K, Pintilie GD, Li S, Schmid MF, Chiu W
Cell Res (2020) 30 pp. 1136-1139 [ DOI: doi:10.1038/s41422-020-00432-2 Pubmed: 33139928 ]
- Apoferritin (480 kDa, Complex from Homo sapiens)
- Sodium ion (22 Da, Ligand)
- Water (18 Da, Ligand)
- Ferritin heavy chain (20 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
Resolution: 3.39 Å
EM Method: Single-particle
Fitted PDBs: 7kip
Q-score: 0.472
Zhang K, Li S, Pintilie G, Chmielewski D, Schmid MF, Simmons G, Jin J, Chiu W
bioRxiv (2020) [ Pubmed: 32817943 DOI: doi:10.1101/2020.08.11.245696 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Hcov-nl63 spike trimer (600 kDa, Complex from Human coronavirus NL63)
- Spike glycoprotein (149 kDa, Protein from HCoV-NL63)
Human Drosha and DGCR8 in complex with Primary MicroRNA (MP/RNA complex) - Active state
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6v5b
Q-score: 0.337
Partin AC, Zhang K, Jeong BC, Herrell E, Li S, Chiu W, Nam Y
Mol Cell (2020) 78 pp. 411- [ Pubmed: 32220646 DOI: doi:10.1016/j.molcel.2020.02.016 ]
- Human drosha and dgcr8 in complex with primary microrna (mp/rna complex) - active state (40 kDa, Complex from Homo sapiens)
- Ribonuclease 3 (118 kDa, Protein from Homo sapiens)
- Microprocessor complex subunit dgcr8 (60 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Pri-mir-16-2 (78-mer) (33 kDa, RNA from Homo sapiens)
Human Drosha and DGCR8 in complex with Primary MicroRNA (MP/RNA complex) - partially docked state
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 6v5c
Q-score: 0.294
Partin AC, Zhang K, Jeong BC, Herrell E, Li S, Chiu W, Nam Y
Mol Cell (2020) 78 pp. 411- [ DOI: doi:10.1016/j.molcel.2020.02.016 Pubmed: 32220646 ]
- Ribonuclease 3 (118 kDa, Protein from Homo sapiens)
- Microprocessor complex subunit dgcr8 (60 kDa, Protein from Homo sapiens)
- Pri-mir-16-2 (66-mer) (33 kDa, RNA from Homo sapiens)
- Human drosha and dgcr8 in complex with primary microrna (mp/rna complex) - partially docked state (40 kDa, Complex from Homo sapiens)
Type I-F CRISPR-Csy complex with its inhibitor AcrF9
Resolution: 2.57 Å
EM Method: Single-particle
Fitted PDBs: 6vqv
Q-score: 0.495
Zhang K, Wang S, Li S, Zhu Y, Pintilie GD, Mou TC, Schmid MF, Huang Z, Chiu W
PNAS (2020) 117 pp. 7176-7182 [ DOI: doi:10.1073/pnas.1922638117 Pubmed: 32170016 ]
- Crispr-associated protein csy1 (49 kDa, Protein from Pseudomonas aeruginosa)
- Crispr-associated protein csy3 (39 kDa, Protein from Pseudomonas aeruginosa)
- Acrf9 (7 kDa, Protein from Pseudomonas aeruginosa)
- Crrna (60-mer) (19 kDa, RNA from Pseudomonas aeruginosa)
- Crispr-associated endonuclease cas6/csy4 (21 kDa, Protein from Pseudomonas aeruginosa)
- Type i-f crispr-csy complex with its inhibitor acrf9 (350 kDa, Complex from Pseudomonas aeruginosa)
- Crispr-associated protein csy2 (36 kDa, Protein from Pseudomonas aeruginosa)