6-Deoxyerythronolide B synthase (DEBS) hybrid module (M3/1) in complex with antibody fragment 1B2
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7m7e
Q-score: 0.449
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ Pubmed: 34735239 DOI: doi:10.1126/science.abi8358 ]
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
- 6-deoxyerythronolide-b synthase erya2, modules 3 and 4,eryai,6-deoxyerythronolide-b synthase erya3, modules 5 and 6 chimera (187 kDa, Protein from Saccharopolyspora erythraea)
- Complex between debs m3/1te and antibody fragment 1b2 (430 kDa, Complex from Saccharopolyspora erythraea)
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)
6-Deoxyerythronolide B synthase (DEBS) module 1 in complex with antibody fragment 1B2: State 1
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7m7f
Q-score: 0.403
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ Pubmed: 34735239 DOI: doi:10.1126/science.abi8358 ]
- N~3~-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-n-(2-sulfanylethyl)-beta-alaninamide (358 Da, Ligand)
- Eryai,6-deoxyerythronolide-b synthase erya3, modules 5 and 6 chimera (188 kDa, Protein from Saccharopolyspora erythraea)
- Complex between debs (3)m1te and antibody fragment 1b2 (480 kDa, Complex from Saccharopolyspora erythraea)
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)
6-Deoxyerythronolide B synthase (DEBS) module 1 in complex with antibody fragment 1B2: State 2
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7m7g
Q-score: 0.181
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ Pubmed: 34735239 DOI: doi:10.1126/science.abi8358 ]
- Complex between debs (3)m1te and antibody fragment 1b2 (480 kDa, Complex from Saccharopolyspora erythraea)
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
- Eryai,6-deoxyerythronolide-b synthase erya3, modules 5 and 6 chimera (188 kDa, Protein from Saccharopolyspora erythraea)
6-Deoxyerythronolide B synthase (DEBS) module 1 in complex with antibody fragment 1B2: State 1'
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7m7h
Q-score: 0.19
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ Pubmed: 34735239 DOI: doi:10.1126/science.abi8358 ]
- Complex between debs (3)m1te and antibody fragment 1b2 (480 kDa, Complex from Saccharopolyspora erythraea)
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
- Eryai,6-deoxyerythronolide-b synthase erya3, modules 5 and 6 chimera (188 kDa, Protein from Saccharopolyspora erythraea)
6-Deoxyerythronolide B synthase (DEBS) module 1 in complex with antibody fragment 1B2 (TE-free)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7m7i
Q-score: 0.304
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ Pubmed: 34735239 DOI: doi:10.1126/science.abi8358 ]
- 1b2 (heavy chain) (25 kDa, Protein from Homo sapiens)
- N~3~-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-n-(2-sulfanylethyl)-beta-alaninamide (358 Da, Ligand)
- Eryai (167 kDa, Protein from Saccharopolyspora erythraea)
- 1b2 (light chain) (26 kDa, Protein from Saccharopolyspora erythraea)
- Complex between debs (3)m1(2) and antibody fragment 1b2 (440 kDa, Complex from Saccharopolyspora erythraea)
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 7m7j
Q-score: 0.166
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ Pubmed: 34735239 DOI: doi:10.1126/science.abi8358 ]
- Complex between debs (3)m1(2) and antibody fragment 1b2 (440 kDa, Complex from Saccharopolyspora erythraea)
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
- Eryai (167 kDa, Protein from Saccharopolyspora erythraea)
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)
Resolution: 1.27 Å
EM Method: Single-particle
Fitted PDBs: 7rrp
Q-score: 0.908
Zhang K, Pintilie GD, Li S, Schmid MF, Chiu W
Cell Res (2020) 30 pp. 1136-1139 [ DOI: doi:10.1038/s41422-020-00432-2 Pubmed: 33139928 ]
- Apoferritin (480 kDa, Complex from Homo sapiens)
- Sodium ion (22 Da, Ligand)
- Water (18 Da, Ligand)
- Ferritin heavy chain (20 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
Molecular basis for the ATPase-powered substrate translocation by the Lon AAA+ protease
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6wqh
Q-score: 0.431
Li S, Hsieh KY, Su SC, Pintilie GD, Zhang K, Chang CI
J Biol Chem (2021) 297 pp. 101239-101239 [ DOI: doi:10.1016/j.jbc.2021.101239 Pubmed: 34563541 ]
- Lon protease (88 kDa, Protein from Meiothermus taiwanensis)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Ig2 substrate (954 Da, Protein from Dictyostelium discoideum)
- Ig2 substrate (Complex from Dictyostelium discoideum)
- Meiothermus taiwanensis lona (mtalona) in a substrate-engaged state (360 kDa, Complex)
- Lon protease (Complex from Meiothermus taiwanensis)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- N-[(1r)-1-(dihydroxyboranyl)-2-phenylethyl]-nalpha-(pyrazin-2-ylcarbonyl)-l-phenylalaninamide (418 Da, Ligand)
Resolution: 1.34 Å
EM Method: Single-particle
Fitted PDBs: 7k3v
Q-score: 0.899
Zhang K, Pintilie GD, Li S, Schmid MF, Chiu W
Cell Res (2020) 30 pp. 1136-1139 [ DOI: doi:10.1038/s41422-020-00432-2 Pubmed: 33139928 ]
- Apoferritin (480 kDa, Complex from Homo sapiens)
- Ferritin heavy chain (20 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Sodium ion (22 Da, Ligand)
Resolution: 1.36 Å
EM Method: Single-particle
Fitted PDBs: 7k3w
Q-score: 0.9
Zhang K, Pintilie GD, Li S, Schmid MF, Chiu W
Cell Res (2020) 30 pp. 1136-1139 [ DOI: doi:10.1038/s41422-020-00432-2 Pubmed: 33139928 ]
- Apoferritin (480 kDa, Complex from Homo sapiens)
- Sodium ion (22 Da, Ligand)
- Water (18 Da, Ligand)
- Ferritin heavy chain (20 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)