Type I-F CRISPR-Csy complex with its inhibitor AcrF9
Resolution: 2.57 Å
EM Method: Single-particle
Fitted PDBs: 6vqv
Q-score: 0.495
Zhang K, Wang S, Li S, Zhu Y, Pintilie GD, Mou TC, Schmid MF, Huang Z, Chiu W
PNAS (2020) 117 pp. 7176-7182 [ DOI: doi:10.1073/pnas.1922638117 Pubmed: 32170016 ]
- Crispr-associated protein csy1 (49 kDa, Protein from Pseudomonas aeruginosa)
- Crispr-associated protein csy3 (39 kDa, Protein from Pseudomonas aeruginosa)
- Acrf9 (7 kDa, Protein from Pseudomonas aeruginosa)
- Crrna (60-mer) (19 kDa, RNA from Pseudomonas aeruginosa)
- Crispr-associated endonuclease cas6/csy4 (21 kDa, Protein from Pseudomonas aeruginosa)
- Type i-f crispr-csy complex with its inhibitor acrf9 (350 kDa, Complex from Pseudomonas aeruginosa)
- Crispr-associated protein csy2 (36 kDa, Protein from Pseudomonas aeruginosa)
Type I-F CRISPR-Csy complex with its inhibitor AcrF8
Resolution: 3.42 Å
EM Method: Single-particle
Fitted PDBs: 6vqw
Q-score: 0.462
Zhang K, Wang S, Li S, Zhu Y, Pintilie GD, Mou TC, Schmid MF, Huang Z, Chiu W
PNAS (2020) 117 pp. 7176-7182 [ DOI: doi:10.1073/pnas.1922638117 Pubmed: 32170016 ]
- Crrna (40-mer) (19 kDa, RNA from Pseudomonas aeruginosa)
- Acrf8 (10 kDa, Protein from Pseudomonas aeruginosa)
- Crispr-associated protein csy1 (49 kDa, Protein from Pseudomonas aeruginosa)
- Crispr-associated protein csy3 (39 kDa, Protein from Pseudomonas aeruginosa)
- Type i-f crispr-associated protein csy2 (36 kDa, Protein from Pseudomonas aeruginosa)
- Type i-f crispr-csy complex with its inhibitor acrf8 (350 kDa, Complex from Pseudomonas aeruginosa)
- Crispr-associated endonuclease cas6/csy4 (21 kDa, Protein from Pseudomonas aeruginosa)
Type I-F CRISPR-Csy complex with its inhibitor AcrF6
Resolution: 3.15 Å
EM Method: Single-particle
Fitted PDBs: 6vqx
Q-score: 0.433
Zhang K, Wang S, Li S, Zhu Y, Pintilie GD, Mou TC, Schmid MF, Huang Z, Chiu W
PNAS (2020) 117 pp. 7176-7182 [ DOI: doi:10.1073/pnas.1922638117 Pubmed: 32170016 ]
- Crispr-associated endonuclease cas6/csy4 (21 kDa, Protein from Pseudomonas aeruginosa)
- Acrf6 (10 kDa, Protein from Pseudomonas aeruginosa)
- Type i-f crispr-csy complex with its inhibitor acrf6 (350 kDa, Complex from Pseudomonas aeruginosa)
- Crispr-associated protein csy1 (49 kDa, Protein from Pseudomonas aeruginosa)
- Crrna (60-mer) (19 kDa, RNA from Pseudomonas aeruginosa)
- Crispr-associated protein csy3 (39 kDa, Protein from Pseudomonas aeruginosa)
- Type i-f crispr-associated protein csy2 (36 kDa, Protein from Pseudomonas aeruginosa)
Small conformation of ssRNA-bound CRISPR_Csm complex
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6nud
Q-score: 0.254
Guo M, Zhang K, Zhu Y, Pintilie GD, Guan X, Li S, Schmid MF, Ma Z, Chiu W, Huang Z
Cell Res (2019) 29 pp. 305-312 [ DOI: doi:10.1038/s41422-019-0151-x Pubmed: 30814678 ]
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Small conformation of ssrna-bound crispr_csm complex (Complex from Streptococcus thermophilus)
- Crispr type iii-associated ramp protein csm4 (33 kDa, Protein from Streptococcus thermophilus)
- Target ssrna (15 kDa, RNA from Streptococcus thermophilus)
- Crispr type iii-associated ramp protein csm3 (24 kDa, Protein from Streptococcus thermophilus)
- Crispr system cms protein csm2 (14 kDa, Protein from Streptococcus thermophilus)
- Crispr system single-strand-specific deoxyribonuclease cas10/csm1 (subtype iii-a) (86 kDa, Protein from Streptococcus thermophilus)
- Crrna (22 kDa, RNA from Streptococcus thermophilus)
Small conformation of apo CRISPR_Csm complex
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6nue
Q-score: 0.305
Guo M, Zhang K, Zhu Y, Pintilie GD, Guan X, Li S, Schmid MF, Ma Z, Chiu W, Huang Z
Cell Res (2019) 29 pp. 305-312 [ Pubmed: 30814678 DOI: doi:10.1038/s41422-019-0151-x ]
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Crispr system cms protein csm2 (14 kDa, Protein from Streptococcus thermophilus)
- Crispr type iii-associated ramp protein csm4 (33 kDa, Protein from Streptococcus thermophilus)
- Crrna (22 kDa, RNA from Streptococcus thermophilus)
- Crispr system single-strand-specific deoxyribonuclease cas10/csm1 (subtype iii-a) (86 kDa, Protein from Streptococcus thermophilus)
- Crispr type iii-associated ramp protein csm3 (24 kDa, Protein from Streptococcus thermophilus)
- Small conformation of apo crispr_csm complex (Complex from Streptococcus thermophilus)