Resolution: 2.44 Å
EM Method: Single-particle
Fitted PDBs: 7fid
Q-score: 0.399
Li S, Hsieh KY, Kuo CI, Su SC, Huang KF, Zhang K, Chang CI
Sci Adv (2021) 7 pp. eabj9537-eabj9537 [ Pubmed: 34757797 DOI: doi:10.1126/sciadv.abj9537 ]
- Lon protease (90 kDa, Protein from Meiothermus taiwanensis)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Unknown endogenous substrate (1 kDa, Protein from Meiothermus taiwanensis)
- Lon bound to endogenous substrate (600 kDa, Complex from Meiothermus taiwanensis WR-220)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Resolution: 2.36 Å
EM Method: Single-particle
Fitted PDBs: 7fie
Q-score: 0.4
Li S, Hsieh KY, Kuo CI, Su SC, Huang KF, Zhang K, Chang CI
Sci Adv (2021) 7 pp. eabj9537-eabj9537 [ DOI: doi:10.1126/sciadv.abj9537 Pubmed: 34757797 ]
- Unknown endogenous substrate (1 kDa, Protein from Meiothermus taiwanensis WR-220)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Lon bound to endogenous substrate (600 kDa, Complex from Meiothermus taiwanensis WR-220)
- Lon protease (90 kDa, Protein from Meiothermus taiwanensis)
Resolution: 3.28 Å
EM Method: Single-particle
Fitted PDBs: 7fiz
Q-score: 0.311
Li S, Hsieh KY, Kuo CI, Su SC, Huang KF, Zhang K, Chang CI
Sci Adv (2021) 7 pp. eabj9537-eabj9537 [ DOI: doi:10.1126/sciadv.abj9537 Pubmed: 34757797 ]
- Unknown endogenous substrate (1 kDa, Protein from Meiothermus taiwanensis WR-220)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Lon bound to endogenous substrate (600 kDa, Complex from Meiothermus taiwanensis WR-220)
- Lon protease (90 kDa, Protein from Meiothermus taiwanensis)
6-Deoxyerythronolide B synthase (DEBS) hybrid module (M3/1) in complex with antibody fragment 1B2
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7m7e
Q-score: 0.449
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ Pubmed: 34735239 DOI: doi:10.1126/science.abi8358 ]
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
- 6-deoxyerythronolide-b synthase erya2, modules 3 and 4,eryai,6-deoxyerythronolide-b synthase erya3, modules 5 and 6 chimera (187 kDa, Protein from Saccharopolyspora erythraea)
- Complex between debs m3/1te and antibody fragment 1b2 (430 kDa, Complex from Saccharopolyspora erythraea)
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)
6-Deoxyerythronolide B synthase (DEBS) module 1 in complex with antibody fragment 1B2: State 1
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7m7f
Q-score: 0.403
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ Pubmed: 34735239 DOI: doi:10.1126/science.abi8358 ]
- N~3~-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-n-(2-sulfanylethyl)-beta-alaninamide (358 Da, Ligand)
- Eryai,6-deoxyerythronolide-b synthase erya3, modules 5 and 6 chimera (188 kDa, Protein from Saccharopolyspora erythraea)
- Complex between debs (3)m1te and antibody fragment 1b2 (480 kDa, Complex from Saccharopolyspora erythraea)
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)
6-Deoxyerythronolide B synthase (DEBS) module 1 in complex with antibody fragment 1B2: State 2
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7m7g
Q-score: 0.181
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ Pubmed: 34735239 DOI: doi:10.1126/science.abi8358 ]
- Complex between debs (3)m1te and antibody fragment 1b2 (480 kDa, Complex from Saccharopolyspora erythraea)
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
- Eryai,6-deoxyerythronolide-b synthase erya3, modules 5 and 6 chimera (188 kDa, Protein from Saccharopolyspora erythraea)
6-Deoxyerythronolide B synthase (DEBS) module 1 in complex with antibody fragment 1B2: State 1'
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7m7h
Q-score: 0.19
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ Pubmed: 34735239 DOI: doi:10.1126/science.abi8358 ]
- Complex between debs (3)m1te and antibody fragment 1b2 (480 kDa, Complex from Saccharopolyspora erythraea)
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
- Eryai,6-deoxyerythronolide-b synthase erya3, modules 5 and 6 chimera (188 kDa, Protein from Saccharopolyspora erythraea)
6-Deoxyerythronolide B synthase (DEBS) module 1 in complex with antibody fragment 1B2 (TE-free)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7m7i
Q-score: 0.304
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ Pubmed: 34735239 DOI: doi:10.1126/science.abi8358 ]
- 1b2 (heavy chain) (25 kDa, Protein from Homo sapiens)
- N~3~-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-n-(2-sulfanylethyl)-beta-alaninamide (358 Da, Ligand)
- Eryai (167 kDa, Protein from Saccharopolyspora erythraea)
- 1b2 (light chain) (26 kDa, Protein from Saccharopolyspora erythraea)
- Complex between debs (3)m1(2) and antibody fragment 1b2 (440 kDa, Complex from Saccharopolyspora erythraea)
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 7m7j
Q-score: 0.166
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ Pubmed: 34735239 DOI: doi:10.1126/science.abi8358 ]
- Complex between debs (3)m1(2) and antibody fragment 1b2 (440 kDa, Complex from Saccharopolyspora erythraea)
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
- Eryai (167 kDa, Protein from Saccharopolyspora erythraea)
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7fd4
Q-score: 0.4
Li S, Hsieh KY, Kuo CI, Lee SH, Pintilie GD, Zhang K, Chang CI
Sci Adv (2021) 7 pp. eabj7835-eabj7835 [ Pubmed: 34652947 DOI: doi:10.1126/sciadv.abj7835 ]
- Alpha-s1-casein (1 kDa, Protein from Bos taurus)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Alpha-s1-casein (Complex)
- Lon protease (Complex from Bos taurus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- A complete three-dimensional structure of the lon protease translocating a protein substrate (conformation 1) (370 kDa, Complex from Meiothermus taiwanensis)
- Lon protease (88 kDa, Protein from Meiothermus taiwanensis)
- N-[(1r)-1-(dihydroxyboranyl)-2-phenylethyl]-nalpha-(pyrazin-2-ylcarbonyl)-l-phenylalaninamide (418 Da, Ligand)