cryo-EM structure of NTSR1-GRK2-Galpha(q) complexes 1
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 8jpb
Q-score: 0.448
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ DOI: doi:10.1038/s41586-023-06395-9 Pubmed: 37532940 ]
- Tetrafluoroaluminate ion (102 Da, Ligand)
- Staurosporine (466 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Ntsr1-grk2-galpha(q) complexes 1 (Complex from Homo sapiens)
- Beta-adrenergic receptor kinase 1 (79 kDa, Protein from Bos taurus)
- Nts(8-13) (819 Da, Protein from synthetic construct)
- 2-[{2-(1-fluorocyclopropyl)-4-[4-(2-methoxyphenyl)piperidin-1-yl]quinazolin-6-yl}(methyl)amino]ethan-1-ol (450 Da, Ligand)
- Guanine nucleotide-binding protein g(q) subunit alpha (41 kDa, Protein from Homo sapiens)
- Neurotensin receptor type 1 (46 kDa, Protein from Homo sapiens)
cryo-EM structure of NTSR1-GRK2-Galpha(q) complexes 2
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 8jpc
Q-score: 0.458
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ DOI: doi:10.1038/s41586-023-06395-9 Pubmed: 37532940 ]
- Tetrafluoroaluminate ion (102 Da, Ligand)
- Staurosporine (466 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Ntsr1-grk2-galpha(q) complexes 2 (Complex from Homo sapiens)
- Beta-adrenergic receptor kinase 1 (79 kDa, Protein from Bos taurus)
- Nts(8-13) (819 Da, Protein from synthetic construct)
- 2-[{2-(1-fluorocyclopropyl)-4-[4-(2-methoxyphenyl)piperidin-1-yl]quinazolin-6-yl}(methyl)amino]ethan-1-ol (450 Da, Ligand)
- Guanine nucleotide-binding protein g(q) subunit alpha (41 kDa, Protein from Homo sapiens)
- Neurotensin receptor type 1 (46 kDa, Protein from Homo sapiens)
Focused refinement structure of GRK2 in NTSR1-GRK2-Galpha(q) complexes
Resolution: 2.81 Å
EM Method: Single-particle
Fitted PDBs: 8jpd
Q-score: 0.497
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ DOI: doi:10.1038/s41586-023-06395-9 Pubmed: 37532940 ]
- Staurosporine (466 Da, Ligand)
- Beta-adrenergic receptor kinase 1 (79 kDa, Protein from Bos taurus)
- Focused refinement structure of grk2 in ntsr1-grk2-galpha(q) complexes (Complex from Bos taurus)
Focused refinement structure of Galpha(q) in NTSR1-GRK2-Galpha(q) complexes
Resolution: 2.91 Å
EM Method: Single-particle
Fitted PDBs: 8jpe
Q-score: 0.447
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ DOI: doi:10.1038/s41586-023-06395-9 Pubmed: 37532940 ]
- Tetrafluoroaluminate ion (102 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Focused refinement structure of galpha(q) in ntsr1-grk2-galpha(q) complexes (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(q) subunit alpha (41 kDa, Protein from Homo sapiens)
Focused refiment structure of NTSR1 in NTSR1-GRK2-Galpha(q) complexes
Resolution: 3.02 Å
EM Method: Single-particle
Fitted PDBs: 8jpf
Q-score: 0.479
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ DOI: doi:10.1038/s41586-023-06395-9 Pubmed: 37532940 ]
- Focused refiment structure of ntsr1 in ntsr1-grk2-galpha(q) complexes (Complex from Homo sapiens)
- Nts (819 Da, Protein from synthetic construct)
- 2-[{2-(1-fluorocyclopropyl)-4-[4-(2-methoxyphenyl)piperidin-1-yl]quinazolin-6-yl}(methyl)amino]ethan-1-ol (450 Da, Ligand)
- Neurotensin receptor type 1 (46 kDa, Protein from Homo sapiens)
Resolution: 3.34 Å
EM Method: Single-particle
Fitted PDBs: 8dt8
Q-score: 0.443
Misson Mindrebo L, Liu H, Ozorowski G, Tran Q, Woehl J, Khalek I, Smith JM, Barman S, Zhao F, Keating C, Limbo O, Verma M, Liu J, Stanfield RL, Zhu X, Turner HL, Sok D, Huang PS, Burton DR, Ward AB, Wilson IA, Jardine JG
PNAS (2023) 120 pp. e2216612120-e2216612120 [ Pubmed: 37276407 DOI: doi:10.1073/pnas.2216612120 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Lm18 nanobody (14 kDa, Protein from synthetic construct)
- Lm18/nb136 bispecific tetra-nanobody immunoglobulin in complex with sars-cov-2-6p-mut7 s protein (Complex from Severe acute respiratory syndrome coronavirus 2)
- Spike glycoprotein (141 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Nb136 nanobody (13 kDa, Protein from synthetic construct)
Misson Mindrebo L, Liu H, Ozorowski G, Tran Q, Woehl J, Khalek I, Smith JM, Barman S, Zhao F, Keating C, Limbo O, Verma M, Liu J, Stanfield RL, Zhu X, Turner HL, Sok D, Huang PS, Burton DR, Ward AB, Wilson IA, Jardine JG
PNAS (2023) 120 pp. e2216612120-e2216612120 [ Pubmed: 37276407 DOI: doi:10.1073/pnas.2216612120 ]
Cryo-EM structure of the INSL5-bound human relaxin family peptidereceptor 4 (RXFP4)-Gi complex
Resolution: 3.19 Å
EM Method: Single-particle
Fitted PDBs: 7yj4
Q-score: 0.429
Chen Y, Zhou Q, Wang J, Xu Y, Wang Y, Yan J, Wang Y, Zhu Q, Zhao F, Li C, Chen CW, Cai X, Bathgate RAD, Shen C, Eric Xu H, Yang D, Liu H, Wang MW
Nat Commun (2023) 14 pp. 492-492 [ Pubmed: 36717591 DOI: doi:10.1038/s41467-023-36182-z ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i) subunit alpha-2 (40 kDa, Protein from Homo sapiens)
- Insulin-like peptide insl5 a chain (2 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- G protein (Complex from Homo sapiens)
- Cryo-em structure of the human relaxin family peptide receptor 4 in complex with insl5 and g protein (Complex)
- Insulin-like peptide insl5 b chain (2 kDa, Protein from Homo sapiens)
- Human relaxin family peptide receptor 4 (Complex from Homo sapiens)
- Relaxin-3 receptor 2 (41 kDa, Protein from Homo sapiens)
- Insl5 (Complex from Homo sapiens)
- Scfv16 (Complex from synthetic construct)
Cryo-EM structure of the compound 4-bound human relaxin family peptide receptor 4 (RXFP4)-Gi complex
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 7yk6
Q-score: 0.419
Chen Y, Zhou Q, Wang J, Xu Y, Wang Y, Yan J, Wang Y, Zhu Q, Zhao F, Li C, Chen CW, Cai X, Bathgate RAD, Shen C, Eric Xu H, Yang D, Liu H, Wang MW
Nat Commun (2023) 14 pp. 492-492 [ DOI: doi:10.1038/s41467-023-36182-z Pubmed: 36717591 ]
- Relaxin-3 receptor 2 (41 kDa, Protein from Homo sapiens)
- Cryo-em structure of the human relaxin family peptide receptor 4 in complex with compound 4 and g protein (Complex)
- 1-[2-(4-chlorophenyl)ethyl]-3-[(7-ethyl-5-oxidanyl-1h-indol-3-yl)methylideneamino]guanidine (383 Da, Ligand)
- Relaxin family peptide receptor 4 (Complex from Homo sapiens)
- Scfv16 (26 kDa, Protein from synthetic construct)
- Scfv16 (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i) subunit alpha-2 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- G protein (Complex from Homo sapiens)
Cryo-EM structure of the DC591053-bound human relaxin family peptide receptor 4 (RXFP4)-Gi complex
Resolution: 2.75 Å
EM Method: Single-particle
Fitted PDBs: 7yk7
Q-score: 0.451
Chen Y, Zhou Q, Wang J, Xu Y, Wang Y, Yan J, Wang Y, Zhu Q, Zhao F, Li C, Chen CW, Cai X, Bathgate RAD, Shen C, Eric Xu H, Yang D, Liu H, Wang MW
Nat Commun (2023) 14 pp. 492-492 [ DOI: doi:10.1038/s41467-023-36182-z Pubmed: 36717591 ]
- Cryo-em structure of the human relaxin family peptide receptor 4 in complex with dc591053 and g protein (Complex)
- [(1s)-7-ethoxy-6-methoxy-1-[2-(5-methoxy-1h-indol-3-yl)ethyl]-3,4-dihydro-1h-isoquinolin-2-yl]-morpholin-4-yl-methanone (493 Da, Ligand)
- Relaxin family peptide receptor 4 (Complex from Homo sapiens)
- G protein (Complex from Bos taurus)
- Scfv16 (26 kDa, Protein from synthetic construct)
- Scfv16 (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i) subunit alpha-2 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Relaxin-3 receptor 2 (41 kDa, Protein from Homo sapiens)