Electron Microscopy Data Bank

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sample_name:"23S Ribosomal RNA" AND sample_type:"ligand" AND release_date:[2022-01-01T00:00:00Z TO 2022-12-31T23:59:59Z] AND citation_journal_name:"NAT COMMUN"

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EMD-26630

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Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A)

Release date: June 22, 2022
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7unr
Q-score: 0.561

Basu RS, Sherman MB, Gagnon MG

Nat Commun (2022) 13 pp. 3388- [ DOI: doi:10.1038/s41467-022-31129-2 ]

Sample:

30s ribosomal protein s21 (8 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s2 (27 kDa, Protein from Pseudomonas aeruginosa PAO1)
Translation initiation factor if-2 (91 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l4 (21 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l34 (5 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s5 (17 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l23 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
Pseudomonas aeruginosa 70s ribosome initiation complex bound to compact if2-gdp (composite structure i-a) (Complex from Pseudomonas aeruginosa PAO1)
16s ribosomal rna (497 kDa, RNA from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s9 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l6 (19 kDa, Protein from Pseudomonas aeruginosa PAO1)
Water (18 Da, Ligand)
50s ribosomal protein l24 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
Mrna (7 kDa, RNA from Bacteriophage sp.)
23s ribosomal rna (937 kDa, RNA from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l33 (6 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l32 (6 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s15 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l16 (15 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s7 (17 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s12 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s16 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l36 (4 kDa, Protein from Pseudomonas aeruginosa PAO1)
Zinc ion (65 Da, Ligand)
50s ribosomal protein l11 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l14 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s10 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l35 (7 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l17 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
N-formylmethionine (177 Da, Ligand)
50s ribosomal protein l28 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s19 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s6 (16 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l9 (15 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l19 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l13 (16 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s11 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l29 (7 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l18 (12 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s8 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l5 (20 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l25 (21 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l20 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
5s ribosomal rna (38 kDa, RNA from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s4 (23 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s13 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l21 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
Guanosine-5'-diphosphate (443 Da, Ligand)
50s ribosomal protein l30 (6 kDa, Protein from Pseudomonas aeruginosa PAO1)
Magnesium ion (24 Da, Ligand)
30s ribosomal protein s3 (25 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s17 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l3 (22 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l2 (29 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s14 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l27 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l31 (7 kDa, Protein from Pseudomonas aeruginosa PAO1)
P-site initiator trna (24 kDa, RNA from Escherichia coli)
50s ribosomal protein l22 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s18 (8 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s20 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l15 (15 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l10 (17 kDa, Protein from Pseudomonas aeruginosa PAO1)

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EMD-26633

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Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B)

Release date: June 22, 2022
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7unu
Q-score: 0.577

Basu RS, Sherman MB, Gagnon MG

Nat Commun (2022) 13 pp. 3388- [ DOI: doi:10.1038/s41467-022-31129-2 ]

Sample:

30s ribosomal protein s21 (8 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s2 (27 kDa, Protein from Pseudomonas aeruginosa PAO1)
Pseudomonas aeruginosa 70s ribosome initiation complex bound to compact if2-gdp (composite structure i-b) (Complex from Pseudomonas aeruginosa PAO1)
Translation initiation factor if-2 (91 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l4 (21 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l34 (5 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s5 (17 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l23 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
16s ribosomal rna (497 kDa, RNA from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s9 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l6 (19 kDa, Protein from Pseudomonas aeruginosa PAO1)
Water (18 Da, Ligand)
50s ribosomal protein l24 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
Mrna (7 kDa, RNA from Bacteriophage sp.)
23s ribosomal rna (937 kDa, RNA from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l33 (6 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l32 (6 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s15 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l16 (15 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s7 (17 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s12 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s16 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l36 (4 kDa, Protein from Pseudomonas aeruginosa PAO1)
Zinc ion (65 Da, Ligand)
50s ribosomal protein l11 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l14 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s10 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l35 (7 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l17 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
N-formylmethionine (177 Da, Ligand)
50s ribosomal protein l28 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s19 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s6 (16 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l9 (15 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l19 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l13 (16 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s11 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l29 (7 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l18 (12 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s8 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l5 (20 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l25 (21 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l20 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
5s ribosomal rna (38 kDa, RNA from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s4 (23 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s13 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l21 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
Guanosine-5'-diphosphate (443 Da, Ligand)
50s ribosomal protein l30 (6 kDa, Protein from Pseudomonas aeruginosa PAO1)
Magnesium ion (24 Da, Ligand)
30s ribosomal protein s3 (25 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s17 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l3 (22 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l2 (29 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s14 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l27 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l31 (7 kDa, Protein from Pseudomonas aeruginosa PAO1)
P-site initiator trna (24 kDa, RNA from Escherichia coli)
50s ribosomal protein l22 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s18 (8 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s20 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l15 (15 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l10 (17 kDa, Protein from Pseudomonas aeruginosa PAO1)

[Show more]

EMD-26634

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Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A)

Release date: June 22, 2022
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7unv
Q-score: 0.554

Basu RS, Sherman MB, Gagnon MG

Nat Commun (2022) 13 pp. 3388- [ DOI: doi:10.1038/s41467-022-31129-2 ]

Sample:

30s ribosomal protein s10 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l36 (4 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s14 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l31 (7 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l15 (15 kDa, Protein from Pseudomonas aeruginosa PAO1)
Mrna (7 kDa, RNA from Bacteriophage sp.)
30s ribosomal protein s21 (8 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s6 (16 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l32 (6 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s3 (25 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l28 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s17 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s9 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l34 (5 kDa, Protein from Pseudomonas aeruginosa PAO1)
Pseudomonas aeruginosa 70s ribosome initiation complex bound to if2-gdpcp (structure ii-a) (Complex from Pseudomonas aeruginosa PAO1)
N-formylmethionine (177 Da, Ligand)
30s ribosomal protein s4 (23 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s8 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
Magnesium ion (24 Da, Ligand)
50s ribosomal protein l2 (29 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l23 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l19 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l29 (7 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l13 (16 kDa, Protein from Pseudomonas aeruginosa PAO1)
Translation initiation factor if-2 (91 kDa, Protein from Pseudomonas aeruginosa PAO1)
Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
30s ribosomal protein s13 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
16s ribosomal rna (497 kDa, RNA from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s20 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l6 (19 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s19 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s7 (17 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l4 (21 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l10 (17 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l11 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
P-site initiator trna (24 kDa, RNA from Escherichia coli)
30s ribosomal protein s5 (17 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s2 (27 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s15 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s18 (8 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l3 (22 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l18 (12 kDa, Protein from Pseudomonas aeruginosa PAO1)
Zinc ion (65 Da, Ligand)
30s ribosomal protein s12 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l9 (15 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l24 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
23s ribosomal rna (937 kDa, RNA from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l27 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s16 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l16 (15 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l35 (7 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l22 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
Water (18 Da, Ligand)
30s ribosomal protein s11 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l25 (21 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l33 (6 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l14 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l30 (6 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l21 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l17 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l20 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
5s ribosomal rna (38 kDa, RNA from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l5 (20 kDa, Protein from Pseudomonas aeruginosa PAO1)

[Show more]

EMD-26635

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Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B)

Release date: June 22, 2022
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 7unw
Q-score: 0.554

Basu RS, Sherman MB, Gagnon MG

Nat Commun (2022) 13 pp. 3388- [ DOI: doi:10.1038/s41467-022-31129-2 ]

Sample:

30s ribosomal protein s10 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l36 (4 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s14 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l31 (7 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l15 (15 kDa, Protein from Pseudomonas aeruginosa PAO1)
Mrna (7 kDa, RNA from Bacteriophage sp.)
30s ribosomal protein s21 (8 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s6 (16 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l32 (6 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s3 (25 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l28 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s17 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s9 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l34 (5 kDa, Protein from Pseudomonas aeruginosa PAO1)
N-formylmethionine (177 Da, Ligand)
30s ribosomal protein s4 (23 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s8 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
Magnesium ion (24 Da, Ligand)
50s ribosomal protein l2 (29 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l23 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l19 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l29 (7 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l13 (16 kDa, Protein from Pseudomonas aeruginosa PAO1)
Translation initiation factor if-2 (91 kDa, Protein from Pseudomonas aeruginosa PAO1)
Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
30s ribosomal protein s13 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
16s ribosomal rna (497 kDa, RNA from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s20 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l6 (19 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s19 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s7 (17 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l4 (21 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l10 (17 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l11 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
P-site initiator trna (24 kDa, RNA from Escherichia coli)
30s ribosomal protein s5 (17 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s2 (27 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s15 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s18 (8 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l3 (22 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l18 (12 kDa, Protein from Pseudomonas aeruginosa PAO1)
Zinc ion (65 Da, Ligand)
30s ribosomal protein s12 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l9 (15 kDa, Protein from Pseudomonas aeruginosa PAO1)
Pseudomonas aeruginosa 70s ribosome initiation complex bound to if2-gdpcp (structure ii-b) (Complex from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l24 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
23s ribosomal rna (937 kDa, RNA from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l27 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
30s ribosomal protein s16 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l16 (15 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l35 (7 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l22 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
Water (18 Da, Ligand)
30s ribosomal protein s11 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l25 (21 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l33 (6 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l14 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l30 (6 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l21 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l17 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l20 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
5s ribosomal rna (38 kDa, RNA from Pseudomonas aeruginosa PAO1)
50s ribosomal protein l5 (20 kDa, Protein from Pseudomonas aeruginosa PAO1)

[Show more]

EMD-12261

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Structure of the HigB1 toxin mutant K95A from Mycobacterium tuberculosis (Rv1955) and its target, the cspA mRNA, on the E. coli Ribosome.

Release date: March 2, 2022
Resolution: 3.11 Å
EM Method: Single-particle
Fitted PDBs: 7nbu
Q-score: 0.521

Mansour M, Giudice E, Xu X, Akarsu H, Bordes P, Guillet V, Bigot DJ, Slama N, D'urso G, Chat S, Redder P, Falquet L, Mourey L, Gillet R, Genevaux P

Nat Commun (2022) 13 pp. 2641-2641 [ DOI: doi:10.1038/s41467-022-30373-w Pubmed: 35552387 ]

Sample:

23s and 5s rrna (Complex from Escherichia coli K-12)
30s ribosomal proteins (Complex from Escherichia coli K-12)
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli K-12)
E-site trna (589 Da, RNA from Escherichia coli K-12)
30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
50s ribosomal proteins (Complex from Escherichia coli K-12)
M. tuberculosis higb1_k95a mutant toxin and its target, the cspa mrna, on the e. coli ribosome. (Complex from Escherichia coli K-12)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli K-12)
23s ribosomal rna (941 kDa, RNA from Escherichia coli K-12)
E-site trna (Complex from Escherichia coli K-12)
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli K-12)
Probable endoribonuclease higb1 (14 kDa, Protein from Mycobacterium tuberculosis H37Rv)
5s ribosomal rna (38 kDa, RNA from Escherichia coli K-12)
30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l9 (4 kDa, Protein from Escherichia coli K-12)
50s ribosomal subunit (Complex from Escherichia coli K-12)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli K-12)
P-site fmet-trna(fmet) (Complex from Escherichia coli K-12)
P-site fmet-trna(fmet) (24 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli K-12)
30s ribosomal subunit (Complex from Escherichia coli K-12)
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli K-12)
Zinc ion (65 Da, Ligand)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli K-12)
N-formylmethionine (177 Da, Ligand)
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli K-12)
16s ribosomal rna (498 kDa, RNA from Escherichia coli)
Higb1 toxin (Complex from Mycobacterium tuberculosis)
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l33 (5 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli K-12)
Magnesium ion (24 Da, Ligand)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli K-12)
16s rrna (Complex from Escherichia coli K-12)
30s ribosomal protein s2 (24 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli K-12)
Cspa mrna (3 kDa, RNA from Mycobacterium tuberculosis H37Rv)
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l16,50s ribosomal protein l31 (16 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s6 (11 kDa, Protein from Escherichia coli K-12)
Cspa mrna (Complex from Escherichia coli K-12)
50s ribosomal protein l16 (5 kDa, Protein from Escherichia coli K-12)

[Show more]

EMD-13936

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Cryo-EM structure of the ribosome from Encephalitozoon cuniculi

Release date: Feb. 9, 2022
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7qep
Q-score: 0.577

Nicholson D, Salamina M, Panek J, Helena-Bueno K, Brown CR, Hirt RP, Ranson NA, Melnikov SV

Nat Commun (2022) 13 pp. 591- [ DOI: doi:10.1038/s41467-022-28281-0 ]

Sample:

60s ribosomal protein l34 (12 kDa, Protein from Encephalitozoon cuniculi GB-M1)
40s ribosomal protein s10 (11 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l37a (l43) (9 kDa, Protein from Encephalitozoon cuniculi GB-M1)
40s ribosomal protein s20 (14 kDa, Protein from Encephalitozoon cuniculi GB-M1)
40s ribosomal protein s8 (19 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l21 (18 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l6 (19 kDa, Protein from Encephalitozoon cuniculi GB-M1)
Ribosome from encephalitozoon cuniculi (Complex from Encephalitozoon cuniculi)
60s ribosomal protein l23 (16 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l7 (27 kDa, Protein from Encephalitozoon cuniculi GB-M1)
40s ribosomal protein s23 (15 kDa, Protein from Encephalitozoon cuniculi GB-M1)
40s ribosomal protein s12 (14 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l36 (10 kDa, Protein from Encephalitozoon cuniculi GB-M1)
40s ribosomal protein s4 (30 kDa, Protein from Encephalitozoon cuniculi GB-M1)
40s ribosomal protein s18 (17 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l35a (l33) (12 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l20 (21 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l44 (12 kDa, Protein from Encephalitozoon cuniculi GB-M1)
Ecu06_1135 protein (9 kDa, Protein from Encephalitozoon cuniculi GB-M1)
40s ribosomal protein s28 (7 kDa, Protein from Encephalitozoon cuniculi GB-M1)
40s ribosomal protein s3 (24 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l13 (18 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l31 (13 kDa, Protein from Encephalitozoon cuniculi GB-M1)
40s ribosomal protein s0 (27 kDa, Protein from Encephalitozoon cuniculi GB-M1)
Small subunit (Complex from Encephalitozoon cuniculi)
18s ribosomal rna (422 kDa, RNA from Encephalitozoon cuniculi GB-M1)
5s ribosomal rna (38 kDa, RNA from Encephalitozoon cuniculi GB-M1)
40s ribosomal protein s7 (19 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l30 (11 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l8 (25 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l13a (l16) (22 kDa, Protein from Encephalitozoon cuniculi GB-M1)
40s ribosomal protein s24 (15 kDa, Protein from Encephalitozoon cuniculi GB-M1)
Ecu11_0225 protein (7 kDa, Protein from Encephalitozoon cuniculi GB-M1)
40s ribosomal protein s16 (16 kDa, Protein from Encephalitozoon cuniculi GB-M1)
Ribosomal protein l15 (23 kDa, Protein from Encephalitozoon cuniculi GB-M1)
40s ribosomal protein s29 (7 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l22 (12 kDa, Protein from Encephalitozoon cuniculi GB-M1)
40s ribosomal protein s5 (20 kDa, Protein from Encephalitozoon cuniculi GB-M1)
Similarity to monoubiquitin/carboxy-extension protein fusion (17 kDa, Protein from Encephalitozoon cuniculi GB-M1)
Uncharacterized protein ecu01_0250 (19 kDa, Protein from Encephalitozoon cuniculi GB-M1)
40s ribosomal protein s1 (26 kDa, Protein from Encephalitozoon cuniculi GB-M1)
Guanine nucleotide binding protein beta subunit (36 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l5 (32 kDa, Protein from Encephalitozoon cuniculi GB-M1)
Ribosomal protein s15 (16 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l35-1 (14 kDa, Protein from Encephalitozoon cuniculi GB-M1)
Similarity to 60s ribosomal protein l24 (11 kDa, Protein from Encephalitozoon cuniculi GB-M1)
40s ribosomal protein s27 (9 kDa, Protein from Encephalitozoon cuniculi GB-M1)
40s ribosomal protein s26 (11 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l37 (10 kDa, Protein from Encephalitozoon cuniculi GB-M1)
40s ribosomal protein s14 (14 kDa, Protein from Encephalitozoon cuniculi GB-M1)
Ubiquitin/ l40 ribosomal protein fusion (14 kDa, Protein from Encephalitozoon cuniculi GB-M1)
40s ribosomal protein s17 (14 kDa, Protein from Encephalitozoon cuniculi GB-M1)
40s ribosomal protein s2 (26 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l32 (16 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l23a (11 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l3 (42 kDa, Protein from Encephalitozoon cuniculi GB-M1)
40s ribosomal protein s13 (16 kDa, Protein from Encephalitozoon cuniculi GB-M1)
40s ribosomal protein s19 (15 kDa, Protein from Encephalitozoon cuniculi GB-M1)
Ecu06_1215 protein (12 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l18 (22 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l1 (24 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l39 (6 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l27a (17 kDa, Protein from Encephalitozoon cuniculi GB-M1)
Adenosine monophosphate (347 Da, Ligand)
40s ribosomal protein s11 (17 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l4 (37 kDa, Protein from Encephalitozoon cuniculi GB-M1)
5.8s-23s ribosomal rna (807 kDa, RNA from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l10 (25 kDa, Protein from Encephalitozoon cuniculi GB-M1)
40s ribosomal protein s9 (20 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l11 (19 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l9 (20 kDa, Protein from Encephalitozoon cuniculi GB-M1)
40s ribosomal protein s25 (12 kDa, Protein from Encephalitozoon cuniculi GB-M1)
Zinc ion (65 Da, Ligand)
60s ribosomal protein l29 (6 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l19 (20 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l26 (16 kDa, Protein from Encephalitozoon cuniculi GB-M1)
40s ribosomal protein s6 (25 kDa, Protein from Encephalitozoon cuniculi GB-M1)
Spermidine (145 Da, Ligand)
60s ribosomal protein l17 (20 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l7a (23 kDa, Protein from Encephalitozoon cuniculi GB-M1)
60s ribosomal protein l27 (14 kDa, Protein from Encephalitozoon cuniculi GB-M1)
40s ribosomal protein s15a (s22 in yeast) (14 kDa, Protein from Encephalitozoon cuniculi GB-M1)
Large subunit (Complex from Encephalitozoon cuniculi)

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