Bacillus subtilis MutS2-collided disome complex (stalled 70S)
Resolution: 3.57 Å
EM Method: Single-particle
Fitted PDBs: 8r55
Q-score: 0.386
Park EN, Mackens-Kiani T, Berhane R, Esser H, Erdenebat C, Burroughs AM, Berninghausen O, Aravind L, Beckmann R, Green R, Buskirk AR
EMBO J (2024) 43 pp. 484-506 [ Pubmed: 38177497 DOI: doi:10.1038/s44318-023-00010-3 ]
- 30s ribosomal protein s5 (17 kDa, Protein from Bacillus subtilis)
- 16s rrna (1533-mer) (496 kDa, RNA from Bacillus subtilis)
- Large ribosomal subunit protein ul16 (15 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl28 (6 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s9 (14 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s4 (22 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl35 (7 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s12 (15 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul5 (20 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul23 (10 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul6 (19 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul3 (22 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l18 (12 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s18 (8 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s2 (28 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul4 (22 kDa, Protein from Bacillus subtilis)
- Muts2-disome complex from b. subtilis, focus refined, collided 70s (Complex from Bacillus subtilis)
- 30s ribosomal protein s3 (24 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s14 type z (7 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s13 (13 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s17 (10 kDa, Protein from Bacillus subtilis)
- 5s rrna (112-mer) (36 kDa, RNA from Bacillus subtilis)
- 50s ribosomal protein l19 (13 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s7 (17 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul22 (12 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl17 (13 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul29 (7 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l36 (4 kDa, Protein from Bacillus subtilis)
- Trna (77-mer) (24 kDa, RNA from Bacillus subtilis)
- 50s ribosomal protein l15 (15 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl27 (8 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl31 (7 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul2 (30 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl20 (13 kDa, Protein from Bacillus subtilis)
- Trna (73-mer) (23 kDa, RNA from Bacillus subtilis)
- 23s rna (2887-mer) (949 kDa, RNA from Bacillus subtilis)
- Large ribosomal subunit protein bl33 (5 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s15 (10 kDa, Protein from Bacillus subtilis)
- B. subtilis endonuclease muts2 binding the collided ribosome interface (Complex from Bacillus subtilis)
- 30s ribosomal protein s11 (13 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul13 (16 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s10 (11 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul24 (10 kDa, Protein from Bacillus subtilis)
- Endonuclease muts2 (87 kDa, Protein from Bacillus subtilis)
- Mrna (33-mer) (10 kDa, RNA from Bacillus subtilis)
- 50s ribosomal protein l32 (6 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s16 (10 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l14 (13 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul30 (6 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s6 (11 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s8 (14 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s20 (9 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl21 (11 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl34 (5 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s19 (10 kDa, Protein from Bacillus subtilis)
Bacillus subtilis MutS2-collided disome complex (stalled 70S)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8qpp
Q-score: 0.415
Park EN, Mackens-Kiani T, Berhane R, Esser H, Erdenebat C, Burroughs AM, Berninghausen O, Aravind L, Beckmann R, Green R, Buskirk AR
EMBO J (2024) 43 pp. 484-506 [ Pubmed: 38177497 DOI: doi:10.1038/s44318-023-00010-3 ]
- Large ribosomal subunit protein bl34 (5 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l19 (13 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl20 (13 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul30 (6 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l15 (15 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl27 (8 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul3 (22 kDa, Protein from Bacillus subtilis)
- Mrna (33-mer) (10 kDa, RNA from Bacillus subtilis)
- 50s ribosomal protein l36 (4 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s11 (13 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s20 (9 kDa, Protein from Bacillus subtilis)
- Endonuclease muts2 (87 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s9 (14 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s17 (10 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s3 (24 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul16 (15 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l9 (16 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l14 (13 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl21 (11 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s14 type z (7 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s18 (8 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s16 (10 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul29 (7 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s4 (22 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul13 (16 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl17 (13 kDa, Protein from Bacillus subtilis)
- 5s rrna (112-mer) (36 kDa, RNA from Bacillus subtilis)
- Large ribosomal subunit protein ul23 (10 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s5 (17 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s7 (17 kDa, Protein from Bacillus subtilis)
- 16s rrna (1533-mer) (496 kDa, RNA from Bacillus subtilis)
- 30s ribosomal protein s19 (10 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s13 (13 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl35 (7 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s15 (10 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul5 (20 kDa, Protein from Bacillus subtilis)
- Trna (77-mer) (24 kDa, RNA from Bacillus subtilis)
- 30s ribosomal protein s12 (15 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s6 (11 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s10 (11 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl28 (6 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s2 (28 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl31 (7 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l18 (12 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul22 (12 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul4 (22 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul24 (10 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl33 (5 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l32 (6 kDa, Protein from Bacillus subtilis)
- 23s ribosomal rna (949 kDa, RNA from Bacillus subtilis)
- Large ribosomal subunit protein ul6 (19 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul2 (30 kDa, Protein from Bacillus subtilis)
- Muts2-disome complex from b. subtilis, focus refined, stalled 70s (Complex from Bacillus subtilis)
- 30s ribosomal protein s8 (14 kDa, Protein from Bacillus subtilis)
Cryo-EM structure of the E.coli 70S ribosome in complex with the antibiotic Myxovalargin B.
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8b7y
Q-score: 0.556
Koller TO, Scheid U, Kosel T, Herrmann J, Krug D, Boshoff HIM, Beckert B, Evans JC, Schlemmer J, Sloan B, Weiner DM, Via LE, Moosa A, Ioerger TR, Graf M, Zinshteyn B, Abdelshahid M, Nguyen F, Arenz S, Gille F, Siebke M, Seedorf T, Plettenburg O, Green R, Warnke AL, Ullrich J, Warrass R, Barry 3rd CE, Warner DF, Mizrahi V, Kirschning A, Wilson DN, Muller R
J Am Chem Soc (2023) 145 pp. 851-863 [ DOI: doi:10.1021/jacs.2c08816 Pubmed: 36603206 ]
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli K-12)
- 16s ribosomal rna (496 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli K-12)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli K-12)
- N-formylmethionine (177 Da, Ligand)
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli K-12)
- Cryo-em structure of the e.coli 70s ribosome in complex with the antibiotic myxovalargin b. (2 MDa, Complex from Escherichia coli BW25113)
- 30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli K-12)
- P-trna fmet (24 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli K-12)
- Myxovalargin b (1 kDa, Protein from Myxococcus fulvus)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli K-12)
- Spermidine (145 Da, Ligand)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli K-12)
- Water (18 Da, Ligand)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli K-12)
- Messenger rna (935 Da, RNA from Escherichia coli K-12)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli K-12)
Structure of the SmrB-bound E. coli disome - collided 70S ribosome
Resolution: 5.7 Å
EM Method: Single-particle
Fitted PDBs: 7qgr
Q-score: 0.112
Saito K, Kratzat H, Campbell A, Buschauer R, Burroughs AM, Berninghausen O, Aravind L, Green R, Beckmann R, Buskirk AR
Nature (2022) 603 pp. 503-508 [ DOI: doi:10.1038/s41586-022-04416-7 Pubmed: 35264790 ]
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s3 (24 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
- Upf0115 protein yfcn (20 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
- 23s rrna (941 kDa, RNA from Escherichia coli)
- 16s rrna (498 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s6, non-modified isoform (15 kDa, Protein from Escherichia coli)
- Potassium ion (39 Da, Ligand)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l1 (23 kDa, Protein from Escherichia coli)
- P-site trna (24 kDa, RNA from Escherichia coli)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli)
- Water (18 Da, Ligand)
- A-site trna (23 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
- Mrna (221 kDa, RNA from Vibrio alginolyticus)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s1 (61 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
- 5s rrna (38 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l36 (6 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
- Collided ribosome of the smrb-bound disome structure (Complex from Escherichia coli)
- 50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli)
Structure of the SmrB-bound E. coli disome - stalled 70S ribosome
Resolution: 3.37 Å
EM Method: Single-particle
Fitted PDBs: 7qgn
Q-score: 0.181
Saito K, Kratzat H, Campbell A, Buschauer R, Burroughs AM, Berninghausen O, Aravind L, Green R, Beckmann R, Buskirk AR
Nature (2022) 603 pp. 503-508 [ DOI: doi:10.1038/s41586-022-04416-7 Pubmed: 35264790 ]
- Potassium ion (39 Da, Ligand)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli)
- 23s rrna (941 kDa, RNA from Escherichia coli)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s3 (24 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)
- Ribosomal protein s7 (17 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l36 (6 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
- P-site trna (24 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l1 (23 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
- Vemp nascent chain (20 kDa, Protein from Vibrio alginolyticus)
- 30s ribosomal protein s21 (10 kDa, Protein from Escherichia coli)
- 16s rrna (498 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
- Ribosomal protein l17 (14 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
- 5s rrna (38 kDa, RNA from Escherichia coli)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli)
- Stalled/leading ribosome of the smrb-bound disome structure (Complex from Escherichia coli)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli)
- Upf0115 protein yfcn (20 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
- Water (18 Da, Ligand)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli UMEA 3162-1)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli)
- A-site trna (23 kDa, RNA from Escherichia coli)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli)
Structure of the collided E. coli disome - VemP-stalled 70S ribosome
Resolution: 3.97 Å
EM Method: Single-particle
Fitted PDBs: 7qg8
Q-score: 0.145
Saito K, Kratzat H, Campbell A, Buschauer R, Burroughs AM, Berninghausen O, Aravind L, Green R, Beckmann R, Buskirk AR
Nature (2022) 603 pp. 503-508 [ DOI: doi:10.1038/s41586-022-04416-7 Pubmed: 35264790 ]
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli K-12)
- P-site trna (24 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli K-12)
- 16s rrna (498 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli K-12)
- Vemp nascent chain (20 kDa, Protein from Vibrio alginolyticus)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s21 (10 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli K-12)
- Stalled/leading ribosome of the collided disome structure (Complex)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l36 (6 kDa, Protein from Escherichia coli K-12)
- 23s rrna (941 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli K-12)
- Potassium ion (39 Da, Ligand)
- 50s ribosomal protein l1 (23 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s6, non-modified isoform (15 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli K-12)
- Water (18 Da, Ligand)
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s3 (24 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
- A-site trna (23 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli K-12)
- 5s rrna (38 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli K-12)
Structure of the E. coli disome - collided 70S ribosome
Resolution: 4.48 Å
EM Method: Single-particle
Fitted PDBs: 7qgh
Q-score: 0.126
Saito K, Kratzat H, Campbell A, Buschauer R, Burroughs AM, Berninghausen O, Aravind L, Green R, Beckmann R, Buskirk AR
Nature (2022) 603 pp. 503-508 [ DOI: doi:10.1038/s41586-022-04416-7 Pubmed: 35264790 ]
- 30s ribosomal protein s1 (61 kDa, Protein from Escherichia coli K-12)
- P-site trna (24 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli K-12)
- 16s rrna (498 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s2 (27 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s16 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s21 (10 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli K-12)
- 23s rrna (941 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l36 (6 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli K-12)
- Potassium ion (39 Da, Ligand)
- 30s ribosomal protein s10 (12 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l1 (23 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
- Mrna (221 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s6, non-modified isoform (15 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli K-12)
- Water (18 Da, Ligand)
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli K-12)
- Collided ribosome of the disome structure (Complex from Escherichia coli K-12)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l2 (30 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli K-12)
- A-site trna (23 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli K-12)
- 5s rrna (38 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli K-12)
Structure of the B. subtilis disome - stalled 70S ribosome
Resolution: 4.75 Å
EM Method: Single-particle
Fitted PDBs: 7qgu
Q-score: 0.225
Saito K, Kratzat H, Campbell A, Buschauer R, Burroughs AM, Berninghausen O, Aravind L, Green R, Beckmann R, Buskirk AR
Nature (2022) 603 pp. 503-508 [ DOI: doi:10.1038/s41586-022-04416-7 Pubmed: 35264790 ]
- 50s ribosomal protein l24 (11 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l3 (22 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l30 (6 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s5 (17 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l13 (16 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l11 (14 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l35 (7 kDa, Protein from Bacillus subtilis)
- 23s rrna (949 kDa, RNA from Bacillus subtilis)
- 50s ribosomal protein l21 (11 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l20 (13 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s4 (22 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s9 (14 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s12 (15 kDa, Protein from Bacillus subtilis)
- 5s rrna (38 kDa, RNA from Bacillus subtilis)
- P-site trna (24 kDa, RNA from Bacillus subtilis)
- 50s ribosomal protein l33 (5 kDa, Protein from Bacillus subtilis)
- Ribosomal protein l6 (19 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l18 (12 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s18 (8 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l16 (16 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s2 (28 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s3 (24 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l19 (13 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s14 type z (7 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s13 (13 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s17 (10 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l31 (7 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l4 (22 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s7 (17 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l9 (16 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l2 (30 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l36 (4 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l5 (20 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l15 (15 kDa, Protein from Bacillus subtilis)
- Stalled ribosome of the disome structure (Complex from Bacillus subtilis)
- 16s rrna (503 kDa, RNA from Bacillus subtilis)
- 30s ribosomal protein s19 (10 kDa, Protein from Bacillus subtilis)
- Yqzj (11 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l17 (13 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s15 (10 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l29 (7 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s11 (13 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l34 (5 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s10 (11 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l14 (13 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l32 (6 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l22 (12 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l10 (18 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s16 (10 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s6 (11 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s8 (14 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l23 (10 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s20 (9 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l27 (10 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l28 (6 kDa, Protein from Bacillus subtilis)
Structure of the B. subtilis disome - collided 70S ribosome
Resolution: 5.45 Å
EM Method: Single-particle
Fitted PDBs: 7qh4
Q-score: 0.168
Saito K, Kratzat H, Campbell A, Buschauer R, Burroughs AM, Berninghausen O, Aravind L, Green R, Beckmann R, Buskirk AR
Nature (2022) 603 pp. 503-508 [ DOI: doi:10.1038/s41586-022-04416-7 Pubmed: 35264790 ]
- 50s ribosomal protein l24 (11 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l3 (22 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l30 (6 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s5 (17 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l13 (16 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l11 (14 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l35 (7 kDa, Protein from Bacillus subtilis)
- 23s rrna (949 kDa, RNA from Bacillus subtilis)
- 50s ribosomal protein l21 (11 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l20 (13 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s4 (22 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s9 (14 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s12 (15 kDa, Protein from Bacillus subtilis)
- 5s rrna (38 kDa, RNA from Bacillus subtilis)
- 50s ribosomal protein l33 (5 kDa, Protein from Bacillus subtilis)
- Ribosomal protein l6 (19 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l18 (12 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s18 (8 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l16 (16 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s2 (28 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s3 (24 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l19 (13 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s14 type z (7 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s13 (13 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s17 (10 kDa, Protein from Bacillus subtilis)
- Collided ribosome of the disome structure (Complex from Bacillus subtilis)
- 50s ribosomal protein l4 (22 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s7 (17 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l2 (30 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l36 (4 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l5 (20 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l15 (15 kDa, Protein from Bacillus subtilis)
- 16s rrna (503 kDa, RNA from Bacillus subtilis)
- Trna (24 kDa, RNA from Bacillus subtilis)
- 30s ribosomal protein s19 (10 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l17 (13 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s15 (10 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l29 (7 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s11 (13 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l34 (5 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s10 (11 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l14 (13 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l32 (6 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l22 (12 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l10 (18 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s16 (10 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s6 (11 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s8 (14 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l23 (10 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s20 (9 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l27 (10 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l28 (6 kDa, Protein from Bacillus subtilis)