Release factor-dependent ribosome rescue by BrfA in the Gram-positive bacterium Bacillus subtilis
Resolution: 3.06 Å
EM Method: Single-particle
Fitted PDBs: 6szs
Q-score: 0.535
Shimokawa-Chiba N, Muller C, Fujiwara K, Beckert B, Ito K, Wilson DN, Chiba S
Nat Commun (2019) 10 pp. 5397-5397 [ DOI: doi:10.1038/s41467-019-13408-7 Pubmed: 31776341 ]
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli (strain K12))
- Escherichia coli ribosome with p-site trna-pro(cgg, carrying the nascent chain (Complex from Escherichia coli K-12)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli (strain K12))
- Mrna (1 kDa, RNA from Escherichia coli K-12)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli (strain K12))
- Uncharacterized protein yqkk (8 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli (strain K12))
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli (strain K12))
- Bacillus subtilis ribosome rescue factor (brfa; former yqkk) and peptide chain release factor 2 (rf2) (Complex from Bacillus subtilis (strain 168))
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli (strain K12))
- P-site trna-pro(cgg) (24 kDa, RNA from Escherichia coli (strain K12))
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli (strain K12))
- E. coli 70s stalled on truncated mrna in complex bsbrfa and bsrf2-q251p (Complex)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli (strain K12))
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli (strain K12))
- Peptide chain release factor 2 (41 kDa, Protein from Bacillus subtilis (strain 168))
Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate
Resolution: 2.89 Å
EM Method: Single-particle
Fitted PDBs: 6spf
Q-score: 0.461
Halfon Y, Jimenez-Fernandez A, La Rosa R, Espinosa Portero R, Krogh Johansen H, Matzov D, Eyal Z, Bashan A, Zimmerman E, Belousoff M, Molin S, Yonath A
PNAS (2019) 116 pp. 22275-22281 [ Pubmed: 31611393 DOI: doi:10.1073/pnas.1909831116 ]
- 50s ribosomal protein l13 (15 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s2 (25 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l15 (15 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s7 (17 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s5 (15 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l35 (7 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s10 (10 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l24 (11 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l9 (8 kDa, Protein from Pseudomonas aeruginosa)
- 5s rrna (37 kDa, RNA from Pseudomonas aeruginosa)
- 30s ribosomal protein s16 (8 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l5 (19 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l18 (12 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s13 (12 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l22 (11 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s11 (12 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l2 (29 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l30 (6 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s3 (23 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s9 (14 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l17 (13 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s19 (9 kDa, Protein from Pseudomonas aeruginosa)
- 16s rrna (493 kDa, RNA from Pseudomonas aeruginosa)
- 50s ribosomal protein l23 (10 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l20 (13 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s15 (9 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s12 (13 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l36 (4 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l33 (5 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l25 (20 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l11 (14 kDa, Protein from Pseudomonas aeruginosa)
- Ribosomal protein ul29 (6 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l16 (15 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s6 (11 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l14 (13 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s4 (23 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s21 (4 kDa, Protein from Pseudomonas aeruginosa)
- Pseudomonas aeruginosa 70s ribosome from a clinical isolate (Complex from Pseudomonas aeruginos)
- Pseudomonas aeruginosa strain pao1 23s ribosomal rna (935 kDa, RNA from Pseudomonas aeruginosa)
- 30s ribosomal protein s17 (8 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l32 (6 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s20 (9 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l31 (3 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s18 (6 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l27 (8 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l34 (5 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l19 (12 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s8 (14 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s14 (11 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l28 (8 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l21 (11 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l4 (21 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l3 (22 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l6 (18 kDa, Protein from Pseudomonas aeruginosa)
Pseudomonas aeruginosa 30s ribosome from an aminoglycoside resistant clinical isolate
Resolution: 2.95 Å
EM Method: Single-particle
Fitted PDBs: 6spc
Q-score: 0.444
Halfon Y, Jimenez-Fernandez A, La Rosa R, Espinosa Portero R, Krogh Johansen H, Matzov D, Eyal Z, Bashan A, Zimmerman E, Belousoff M, Molin S, Yonath A
PNAS (2019) 116 pp. 22275-22281 [ Pubmed: 31611393 DOI: doi:10.1073/pnas.1909831116 ]
- 30s ribosomal protein s5 (15 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s7 (17 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s10 (10 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s16 (8 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s8 (13 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s11 (12 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s3 (23 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s9 (14 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s13 (12 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s19 (9 kDa, Protein from Pseudomonas aeruginosa)
- 16s rrna (492 kDa, RNA from Pseudomonas aeruginosa)
- 30s ribosomal protein s4 (23 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s15 (9 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s12 (13 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s6 (11 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s21 (4 kDa, Protein from Pseudomonas aeruginosa)
- Pseudomonas aeruginosa 70s ribosome from a clinical isolate (Complex from Pseudomonas aeruginos)
- 30s ribosomal protein s17 (8 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s2 (24 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s20 (9 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s18 (6 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s14 (11 kDa, Protein from Pseudomonas aeruginosa)
Pseudomonas aeruginosa 30s ribosome from a clinical isolate
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6spe
Q-score: 0.46
Halfon Y, Jimenez-Fernandez A, La Rosa R, Espinosa Portero R, Krogh Johansen H, Matzov D, Eyal Z, Bashan A, Zimmerman E, Belousoff M, Molin S, Yonath A
PNAS (2019) 116 pp. 22275-22281 [ Pubmed: 31611393 DOI: doi:10.1073/pnas.1909831116 ]
- 30s ribosomal protein s7 (17 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s10 (10 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s20 (9 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s16 (8 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s13 (12 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s11 (12 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s6 (12 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s9 (14 kDa, Protein from Pseudomonas aeruginosa)
- 16s ribosomal rna (494 kDa, RNA from Pseudomonas aeruginosa)
- 30s ribosomal protein s19 (9 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s5 (16 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s21 (7 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s3 (23 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s18 (8 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s2 (26 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s4 (23 kDa, Protein from Pseudomonas aeruginosa)
- Pseudomonas aeruginosa 70s ribosome from a clinical isolate (Complex from Pseudomonas aeruginos)
- 30s ribosomal protein s17 (8 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s8 (14 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s15 (9 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s14 (11 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s12 (13 kDa, Protein from Pseudomonas aeruginosa)
Pseudomonas aeruginosa 70s ribosome from a clinical isolate
Resolution: 3.34 Å
EM Method: Single-particle
Fitted PDBs: 6spg
Q-score: 0.468
Halfon Y, Jimenez-Fernandez A, La Rosa R, Espinosa Portero R, Krogh Johansen H, Matzov D, Eyal Z, Bashan A, Zimmerman E, Belousoff M, Molin S, Yonath A
PNAS (2019) 116 pp. 22275-22281 [ DOI: doi:10.1073/pnas.1909831116 Pubmed: 31611393 ]
- Pseudomonas aeruginosa 70s ribosome from a clinical isolate (Complex from Pseudomonas aeruginos)
- 50s ribosomal protein l4 (21 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l31 (3 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l33 (5 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l32 (6 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l2 (29 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s2 (26 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l11 (14 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s21 (7 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s5 (16 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s8 (14 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s17 (8 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l17 (13 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s10 (10 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l21 (11 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l9 (8 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l23 (10 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s20 (9 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s7 (17 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s19 (9 kDa, Protein from Pseudomonas aeruginosa)
- Ribosomal protein ul29 (6 kDa, Protein from Pseudomonas aeruginosa)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l14 (13 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l6 (18 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l27 (8 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l25 (20 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l15 (15 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l5 (19 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l34 (5 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l36 (4 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l19 (12 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s11 (12 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s16 (8 kDa, Protein from Pseudomonas aeruginosa)
- 5s ribosomal rna (37 kDa, RNA from Pseudomonas aeruginosa)
- 50s ribosomal protein l13 (15 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l22 (11 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s4 (23 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s14 (11 kDa, Protein from Pseudomonas aeruginosa)
- 16s ribosomal rna (494 kDa, RNA from Pseudomonas aeruginosa)
- 30s ribosomal protein s15 (9 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s6 (12 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s18 (8 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l20 (13 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l35 (7 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s12 (13 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l24 (11 kDa, Protein from Pseudomonas aeruginosa)
- 23s ribosomal rna (935 kDa, RNA from Pseudomonas aeruginosa)
- 30s ribosomal protein s9 (14 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l18 (12 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l16 (15 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s13 (12 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l3 (22 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l30 (6 kDa, Protein from Pseudomonas aeruginosa)
- 30s ribosomal protein s3 (23 kDa, Protein from Pseudomonas aeruginosa)
- 50s ribosomal protein l28 (8 kDa, Protein from Pseudomonas aeruginosa)
Non-rotated ribosome (Structure I)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6ofx
Q-score: 0.335
Svidritskiy E, Demo G, Loveland AB, Xu C, Korostelev AA
eLife (2019) 8 [ DOI: doi:10.7554/eLife.46850 Pubmed: 31513010 ]
- 30s ribosomal protein s2 (25 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli (strain K12))
- 16s ribosomal rna (498 kDa, RNA from Escherichia coli)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli (strain K12))
- Mrna (8 kDa, RNA from synthetic construct)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l27 (8 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli (strain K12))
- N-formylmethionine (177 Da, Ligand)
- 30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli (strain K12))
- Trnafmet (24 kDa, RNA from Escherichia coli)
- Trnafmet bound to a non-rotated e. coli ribosome (2 MDa, Complex from Escherichia coli (strain K12))
- 30s ribosomal protein s6 (11 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s21 (7 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli (strain K12))
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l1 (23 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l33 (5 kDa, Protein from Escherichia coli (strain K12))
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli (strain K12))
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6og7
Q-score: 0.349
Svidritskiy E, Demo G, Loveland AB, Xu C, Korostelev AA
eLife (2019) 8 [ Pubmed: 31513010 DOI: doi:10.7554/eLife.46850 ]
- Peptide chain release factor rf2 (42 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l33 (5 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l27 (8 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli (strain K12))
- Trnafmet and rf2 bound to a non-rotated e. coli ribosome (2 MDa, Complex from Escherichia coli (strain K12))
- 30s ribosomal protein s2 (25 kDa, Protein from Escherichia coli (strain K12))
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l1 (23 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli (strain K12))
- Mrna (8 kDa, RNA from synthetic construct)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s21 (7 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli (strain K12))
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli (strain K12))
- 16s ribosomal rna (498 kDa, RNA from Escherichia coli)
- 30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli (strain K12))
- Trnafmet (24 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s6 (11 kDa, Protein from Escherichia coli (strain K12))
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6ogf
Q-score: 0.328
Svidritskiy E, Demo G, Loveland AB, Xu C, Korostelev AA
eLife (2019) 8 [ Pubmed: 31513010 DOI: doi:10.7554/eLife.46850 ]
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
- Peptide chain release factor 2 (42 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
- Mrna (8 kDa, RNA from synthetic construct)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
- Trnafmet and rf2 bound to a non-rotated e. coli ribosome (2 MDa, Complex from Escherichia coli)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l1 (24 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
- Trnafmet (24 kDa, RNA from Escherichia coli)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli)
- 16s ribosomal rna (498 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 6ogg
Q-score: 0.224
Svidritskiy E, Demo G, Loveland AB, Xu C, Korostelev AA
eLife (2019) 8 [ DOI: doi:10.7554/eLife.46850 Pubmed: 31513010 ]
- Peptide chain release factor 2 (42 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
- Trnafmet and rf2 bound to a rotated e. coli ribosome (2 MDa, Complex from Escherichia coli)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli)
- Mrna (8 kDa, RNA from synthetic construct)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l1 (24 kDa, Protein from Escherichia coli)
- 16s ribosomal rna (498 kDa, RNA from Escherichia coli)
- Trnafmet (24 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
70S termination complex with RF2 bound to the UAG codon. Rotated ribosome conformation (Structure V)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6ogi
Q-score: 0.321
Svidritskiy E, Demo G, Loveland AB, Xu C, Korostelev AA
eLife (2019) 8 [ DOI: doi:10.7554/eLife.46850 Pubmed: 31513010 ]
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli)
- Mrna (8 kDa, RNA from synthetic construct)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli)
- Trnafmet bound to a rotated e. coli ribosome (2 MDa, Complex from Escherichia coli)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l1 (24 kDa, Protein from Escherichia coli)
- 16s ribosomal rna (498 kDa, RNA from Escherichia coli)
- Trnafmet (24 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)