Bacillus subtilis MutS2-collided disome complex (stalled 70S)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8qpp
Q-score: 0.415
Park EN, Mackens-Kiani T, Berhane R, Esser H, Erdenebat C, Burroughs AM, Berninghausen O, Aravind L, Beckmann R, Green R, Buskirk AR
EMBO J (2024) 43 pp. 484-506 [ Pubmed: 38177497 DOI: doi:10.1038/s44318-023-00010-3 ]
- Large ribosomal subunit protein bl34 (5 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l19 (13 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl20 (13 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul30 (6 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l15 (15 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl27 (8 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul3 (22 kDa, Protein from Bacillus subtilis)
- Mrna (33-mer) (10 kDa, RNA from Bacillus subtilis)
- 50s ribosomal protein l36 (4 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s11 (13 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s20 (9 kDa, Protein from Bacillus subtilis)
- Endonuclease muts2 (87 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s9 (14 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s17 (10 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s3 (24 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul16 (15 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l9 (16 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l14 (13 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl21 (11 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s14 type z (7 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s18 (8 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s16 (10 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul29 (7 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s4 (22 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul13 (16 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl17 (13 kDa, Protein from Bacillus subtilis)
- 5s rrna (112-mer) (36 kDa, RNA from Bacillus subtilis)
- Large ribosomal subunit protein ul23 (10 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s5 (17 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s7 (17 kDa, Protein from Bacillus subtilis)
- 16s rrna (1533-mer) (496 kDa, RNA from Bacillus subtilis)
- 30s ribosomal protein s19 (10 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s13 (13 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl35 (7 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s15 (10 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul5 (20 kDa, Protein from Bacillus subtilis)
- Trna (77-mer) (24 kDa, RNA from Bacillus subtilis)
- 30s ribosomal protein s12 (15 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s6 (11 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s10 (11 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl28 (6 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s2 (28 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl31 (7 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l18 (12 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul22 (12 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul4 (22 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul24 (10 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl33 (5 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l32 (6 kDa, Protein from Bacillus subtilis)
- 23s ribosomal rna (949 kDa, RNA from Bacillus subtilis)
- Large ribosomal subunit protein ul6 (19 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul2 (30 kDa, Protein from Bacillus subtilis)
- Muts2-disome complex from b. subtilis, focus refined, stalled 70s (Complex from Bacillus subtilis)
- 30s ribosomal protein s8 (14 kDa, Protein from Bacillus subtilis)
Methyltransferase RmtC bound to the 30S ribosomal subunit
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8ghu
Q-score: 0.259
Srinivas P, Nosrati M, Zelinskaya N, Dey D, Comstock LR, Dunham CM, Conn GL
bioRxiv (2023) [ Pubmed: 36993224 DOI: doi:10.1101/2023.03.13.532395 ]
- 30s ribosomal protein s18 (6 kDa, Protein from Escherichia coli)
- 16s rrna (guanine(1405)-n(7))-methyltransferase (Complex from Proteus mirabilis)
- 30s ribosomal protein s5 (15 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
- 16s rrna (guanine(1405)-n(7))-methyltransferase (32 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- 16s rrna (496 kDa, RNA from Escherichia coli)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
- 16s rrna methyltransferase rmtc bound to the 30s ribosomal subunit (Complex from Escherichia coli)
- 30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s13 (10 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s6, non-modified isoform (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
Translating 70S ribosome in the unrotated state (P and E, tRNAs)
Resolution: 1.55 Å
EM Method: Single-particle
Fitted PDBs: 8b0x
Q-score: 0.767
Fromm SA, O'Connor KM, Purdy M, Bhatt PR, Loughran G, Atkins JF, Jomaa A, Mattei S
Nat Commun (2023) 14 pp. 1095-1095 [ Pubmed: 36841832 DOI: doi:10.1038/s41467-023-36742-3 ]
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli B)
- Potassium ion (39 Da, Ligand)
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli B)
- 23s rrna (948 kDa, RNA from Escherichia coli B)
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli B)
- 16s rrna (503 kDa, RNA from Escherichia coli B)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli B)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli B)
- Mrna (1 kDa, RNA from Escherichia coli B)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli B)
- 5s rrna (38 kDa, RNA from Escherichia coli B)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli B)
- 70s ribosome (2 MDa, Complex from Escherichia coli B)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli B)
- Zinc ion (65 Da, Ligand)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli B)
- P-site trna (24 kDa, RNA from Escherichia coli B)
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli B)
- Water (18 Da, Ligand)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli B)
PoxtA-EQ2 antibiotic resistance ABCF bound to E. faecalis 70S ribosome, state I
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7p7r
Q-score: -0.017
Crowe-McAuliffe C, Murina V, Turnbull KJ, Huch S, Kasari M, Takada H, Nersisyan L, Sundsfjord A, Hegstad K, Atkinson GC, Pelechano V, Wilson DN, Hauryliuk V
Nat Commun (2022) 13 pp. 1860-1860 [ DOI: doi:10.1038/s41467-022-29274-9 Pubmed: 35387982 ]
- 50s ribosomal protein l5 (20 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l28 (7 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- Potassium ion (39 Da, Ligand)
- 30s ribosomal protein s4 (23 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- Spectinomycin (332 Da, Ligand)
- 30s ribosomal protein s5 (17 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 1,4-diaminobutane (88 Da, Ligand)
- 30s ribosomal protein s2 (29 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s19 (10 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l15 (15 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l20 (13 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l13 (16 kDa, Protein from Enterococcus faecalis V583)
- 5s rrna (37 kDa, RNA from Enterococcus faecalis V583)
- 50s ribosomal protein l31 type b (10 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s18 (9 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 30s ribosomal protein s17 (10 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- Complex of poxta-eq2 bound to the 70s ribosome from e. faecalis (initiation complex), state i. (2 MDa, Complex)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s7 (17 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l29 (7 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l19 (13 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l33 3 (5 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l4 (22 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s10 (11 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 23s rrna (944 kDa, RNA from Enterococcus faecalis V583)
- Trna-fmet (24 kDa, RNA from Enterococcus faecalis V583)
- Mrna (6 kDa, RNA from Enterococcus faecalis V583)
- Are-abc-f family resistance factor poxta (70 kDa, Complex from Enterococcus faecalis)
- 50s ribosomal protein l6 (19 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l18 (12 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s16 (10 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l34 (5 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s6 (11 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s9 (14 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s20 (8 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 16s rrna (504 kDa, RNA from Enterococcus faecalis V583)
- 50s ribosomal protein l30 (6 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l35 (7 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s13 (13 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l24 (11 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 70s ribosome (2 MDa, Complex from Enterococcus faecalis)
- 50s ribosomal protein l17 (14 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l2 (30 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l27 (10 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l22 (12 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s11 (13 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s14 type z (7 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l1 (24 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l32-3 (6 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- Are-abc-f family resistance factor poxta (67 kDa, Protein from Enterococcus faecalis V583)
- 50s ribosomal protein l21 (11 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s12 (15 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s3 (24 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s8 (14 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s15 (10 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l36 (4 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l14 (13 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l23 (11 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l3 (22 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l16 (16 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
E. faecalis 70S ribosome bound by PoxtA-EQ2, high-resolution combined volume
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7p7q
Q-score: 0.572
Crowe-McAuliffe C, Murina V, Turnbull KJ, Huch S, Kasari M, Takada H, Nersisyan L, Sundsfjord A, Hegstad K, Atkinson GC, Pelechano V, Wilson DN, Hauryliuk V
Nat Commun (2022) 13 pp. 1860-1860 [ DOI: doi:10.1038/s41467-022-29274-9 Pubmed: 35387982 ]
- 30s ribosomal protein s19 (10 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s5 (17 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l33 3 (6 kDa, Protein from Enterococcus faecalis)
- Fmet-trna (24 kDa, RNA from Enterococcus faecalis)
- Are-abc-f family resistance factor poxta (67 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s16 (10 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l22 (12 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l34 (5 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l13 (16 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s7 (17 kDa, Protein from Enterococcus faecalis)
- Complex of poxta-eq2 bound to the 70s ribosome from e. faecalis (initiation complex), state i. (Complex)
- 30s ribosomal protein s17 (10 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l29 (7 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l14 (13 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l24 (11 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s2 (29 kDa, Protein from Enterococcus faecalis)
- 70s ribosome (Complex from Enterococcus faecalis)
- 30s ribosomal protein s8 (14 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s11 (13 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s15 (10 kDa, Protein from Enterococcus faecalis)
- Potassium ion (39 Da, Ligand)
- 50s ribosomal protein l31 type b (10 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l15 (15 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l30 (6 kDa, Protein from Enterococcus faecalis)
- Zinc ion (65 Da, Ligand)
- Mrna (6 kDa, RNA from Enterococcus faecalis)
- 30s ribosomal protein s13 (13 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l6 (19 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l21 (11 kDa, Protein from Enterococcus faecalis)
- 1,4-diaminobutane (88 Da, Ligand)
- 50s ribosomal protein l16 (16 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l28 (7 kDa, Protein from Enterococcus faecalis)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 50s ribosomal protein l23 (11 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l19 (13 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l35 (7 kDa, Protein from Enterococcus faecalis)
- 5s rrna (37 kDa, RNA from Enterococcus faecalis)
- 30s ribosomal protein s18 (9 kDa, Protein from Enterococcus faecalis)
- Water (18 Da, Ligand)
- 50s ribosomal protein l2 (30 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l18 (12 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l3 (22 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s3 (24 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s12 (15 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l5 (20 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l32-3 (6 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s10 (11 kDa, Protein from Enterococcus faecalis)
- Magnesium ion (24 Da, Ligand)
- Spectinomycin (332 Da, Ligand)
- 50s ribosomal protein l1 (24 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s4 (23 kDa, Protein from Enterococcus faecalis)
- 16s rrna (504 kDa, RNA from Enterococcus faecalis)
- 50s ribosomal protein l36 (4 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s6 (11 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s14 type z (7 kDa, Protein from Enterococcus faecalis)
- 23s rrna (943 kDa, RNA from Enterococcus faecalis)
- 30s ribosomal protein s9 (14 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l4 (22 kDa, Protein from Enterococcus faecalis)
- Poxa (Complex from Enterococcus faecalis)
- 50s ribosomal protein l27 (10 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l20 (13 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s20 (8 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l17 (14 kDa, Protein from Enterococcus faecalis)
PoxtA-EQ2 antibiotic resistance ABCF bound to E. faecalis 70S ribosome, state II
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7p7s
Q-score: 0.526
Crowe-McAuliffe C, Murina V, Turnbull KJ, Huch S, Kasari M, Takada H, Nersisyan L, Sundsfjord A, Hegstad K, Atkinson GC, Pelechano V, Wilson DN, Hauryliuk V
Nat Commun (2022) 13 pp. 1860-1860 [ DOI: doi:10.1038/s41467-022-29274-9 Pubmed: 35387982 ]
- 30s ribosomal protein s19 (10 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s11 (13 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s5 (17 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l33 (6 kDa, Protein from Enterococcus faecalis)
- Are-abc-f family resistance factor poxta (67 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s16 (10 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l22 (12 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l34 (5 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l13 (16 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s7 (17 kDa, Protein from Enterococcus faecalis)
- 16s rrna (504 kDa, RNA from Enterococcus faecalis)
- 30s ribosomal protein s17 (10 kDa, Protein from Streptococcus faecalis)
- 23s rrna (944 kDa, RNA from Enterococcus faecalis)
- 50s ribosomal protein l29 (7 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l14 (13 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l24 (11 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s2 (29 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s8 (14 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s15 (10 kDa, Protein from Enterococcus faecalis)
- Potassium ion (39 Da, Ligand)
- 50s ribosomal protein l31 type b (10 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l15 (15 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l30 (6 kDa, Protein from Enterococcus faecalis)
- Zinc ion (65 Da, Ligand)
- Mrna (6 kDa, RNA from Enterococcus faecalis)
- 30s ribosomal protein s13 (13 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l6 (19 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l21 (11 kDa, Protein from Enterococcus faecalis)
- Are-abc-f family resistance factor poxta (Complex from Enterococcus faecalis)
- 1,4-diaminobutane (88 Da, Ligand)
- 50s ribosomal protein l28 (7 kDa, Protein from Enterococcus faecalis)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 50s ribosomal protein l23 (11 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l19 (13 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l35 (7 kDa, Protein from Enterococcus faecalis)
- 70s ribosome (Complex from Enterococcus faecalis)
- 5s rrna (37 kDa, RNA from Enterococcus faecalis)
- 30s ribosomal protein s18 (9 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l32 (6 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l16 (16 kDa, Protein from Streptococcus faecalis)
- 50s ribosomal protein l18 (12 kDa, Protein from Enterococcus faecalis)
- Complex of poxta-eq2 bound to the 70s ribosome from e. faecalis (initiation complex), state ii. (Complex)
- 50s ribosomal protein l3 (22 kDa, Protein from Enterococcus faecalis)
- Trna-fmet (25 kDa, RNA from Enterococcus faecalis)
- 50s ribosomal protein l5 (20 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s3 (24 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s10 (11 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l2 (30 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s12 (15 kDa, Protein from Enterococcus faecalis)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l1 (24 kDa, Protein from Enterococcus faecalis)
- Spectinomycin (332 Da, Ligand)
- 30s ribosomal protein s4 (23 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l36 (4 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s6 (11 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s14 type z (7 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s9 (14 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l4 (22 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l27 (10 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l20 (13 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s20 (8 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l17 (14 kDa, Protein from Enterococcus faecalis)
PoxtA-EQ2 antibiotic resistance ABCF bound to E. faecalis 70S ribosome, state III
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7p7t
Q-score: 0.534
Crowe-McAuliffe C, Murina V, Turnbull KJ, Huch S, Kasari M, Takada H, Nersisyan L, Sundsfjord A, Hegstad K, Atkinson GC, Pelechano V, Wilson DN, Hauryliuk V
Nat Commun (2022) 13 pp. 1860-1860 [ DOI: doi:10.1038/s41467-022-29274-9 Pubmed: 35387982 ]
- 30s ribosomal protein s19 (10 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s11 (13 kDa, Protein from Enterococcus faecalis)
- A-site trna (24 kDa, RNA from Escherichia coli)
- 30s ribosomal protein s5 (17 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l33 (6 kDa, Protein from Enterococcus faecalis)
- Are-abc-f family resistance factor poxta (67 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s16 (10 kDa, Protein from Enterococcus faecalis)
- Mrna (6 kDa, RNA from Enterococcus faecalis)
- 50s ribosomal protein l22 (12 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l34 (5 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l13 (16 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s7 (17 kDa, Protein from Enterococcus faecalis)
- Trna-fmet (24 kDa, RNA from Enterococcus faecalis)
- 70s ribosome (Cellular component from Enterococcus faecalis)
- 16s rrna (504 kDa, RNA from Enterococcus faecalis)
- 30s ribosomal protein s17 (10 kDa, Protein from Enterococcus faecalis)
- 23s rrna (944 kDa, RNA from Enterococcus faecalis)
- 50s ribosomal protein l29 (7 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l14 (13 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l24 (11 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s2 (29 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s8 (14 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s15 (10 kDa, Protein from Enterococcus faecalis)
- A-site trna (Complex from Escherichia coli)
- 50s ribosomal protein l31 type b (10 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l15 (15 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l30 (6 kDa, Protein from Enterococcus faecalis)
- Potassium ion (39 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Complex of poxta-eq2 bound to the 70s ribosome from e. faecalis (initiation complex), state iii. (Complex)
- 30s ribosomal protein s13 (13 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l6 (19 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l21 (11 kDa, Protein from Enterococcus faecalis)
- Are-abc-f family resistance factor poxta (Complex from Enterococcus faecalis)
- 1,4-diaminobutane (88 Da, Ligand)
- 50s ribosomal protein l16 (16 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l28 (7 kDa, Protein from Enterococcus faecalis)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 50s ribosomal protein l23 (11 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l19 (13 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l35 (7 kDa, Protein from Enterococcus faecalis)
- 5s rrna (37 kDa, RNA from Enterococcus faecalis)
- 30s ribosomal protein s18 (9 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l32 (6 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l18 (12 kDa, Protein from Enterococcus faecalis)
- Water (18 Da, Ligand)
- 50s ribosomal protein l3 (22 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s3 (24 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s12 (15 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l5 (20 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s10 (11 kDa, Protein from Enterococcus faecalis)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l2 (30 kDa, Protein from Enterococcus faecalis)
- Spectinomycin (332 Da, Ligand)
- 50s ribosomal protein l1 (24 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s4 (23 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l36 (4 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s6 (11 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s14 type z (7 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s9 (14 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l4 (22 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l27 (10 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l20 (13 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s20 (8 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l17 (14 kDa, Protein from Enterococcus faecalis)
E. faecalis 70S ribosome with P-tRNA, state IV
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7p7u
Q-score: 0.526
Crowe-McAuliffe C, Murina V, Turnbull KJ, Huch S, Kasari M, Takada H, Nersisyan L, Sundsfjord A, Hegstad K, Atkinson GC, Pelechano V, Wilson DN, Hauryliuk V
Nat Commun (2022) 13 pp. 1860-1860 [ DOI: doi:10.1038/s41467-022-29274-9 Pubmed: 35387982 ]
- 30s ribosomal protein s19 (10 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s11 (13 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s5 (17 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l33 (6 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s16 (10 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l22 (12 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l34 (5 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l13 (16 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s7 (17 kDa, Protein from Enterococcus faecalis)
- Trna-fmet (24 kDa, RNA from Enterococcus faecalis)
- 16s rrna (504 kDa, RNA from Enterococcus faecalis)
- 30s ribosomal protein s17 (10 kDa, Protein from Streptococcus faecalis)
- 23s rrna (944 kDa, RNA from Enterococcus faecalis)
- 50s ribosomal protein l29 (7 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l14 (13 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l24 (11 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s2 (29 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s8 (14 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s15 (10 kDa, Protein from Enterococcus faecalis)
- Potassium ion (39 Da, Ligand)
- 50s ribosomal protein l31 type b (10 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l15 (15 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l30 (6 kDa, Protein from Enterococcus faecalis)
- Zinc ion (65 Da, Ligand)
- Mrna (6 kDa, RNA from Enterococcus faecalis)
- 30s ribosomal protein s13 (13 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l6 (19 kDa, Protein from Enterococcus faecalis)
- 70s ribosome from e. faecalis with p-trna (initiation complex), state iv. (Complex from Enterococcus faecalis)
- 50s ribosomal protein l21 (11 kDa, Protein from Enterococcus faecalis)
- 1,4-diaminobutane (88 Da, Ligand)
- 50s ribosomal protein l16 (16 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l28 (7 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l23 (11 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l19 (13 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l35 (7 kDa, Protein from Enterococcus faecalis)
- 5s rrna (37 kDa, RNA from Enterococcus faecalis)
- 30s ribosomal protein s18 (9 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l32 (6 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l18 (12 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l3 (22 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s3 (24 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s12 (15 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l5 (20 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s10 (11 kDa, Protein from Enterococcus faecalis)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l2 (30 kDa, Protein from Enterococcus faecalis)
- Spectinomycin (332 Da, Ligand)
- 30s ribosomal protein s4 (23 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l36 (4 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s6 (11 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s14 type z (7 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s9 (14 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l4 (22 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l27 (10 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l20 (13 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s20 (8 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l17 (14 kDa, Protein from Enterococcus faecalis)