Electron Microscopy Data Bank

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Wheat 80S ribosome pausing on AUG-Stop with cycloheximide

Release date: Feb. 21, 2024
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8ipb
Q-score: 0.466

Tanaka M, Yokoyama T, Saito H, Nishimoto M, Tsuda K, Sotta N, Shigematsu H, Shirouzu M, Iwasaki S, Ito T, Fujiwara T

Nat Chem Biol (2024) 20 pp. 605-614 [ DOI: doi:10.1038/s41589-023-01513-0 Pubmed: 38267667 ]

Sample:

60s ribosomal protein ul30 (28 kDa, Protein from Triticum aestivum)
60s ribosomal protein el40 (14 kDa, Protein from Triticum aestivum)
60s ribosomal protein el14 (15 kDa, Protein from Triticum aestivum)
60s ribosomal protein ul22 (19 kDa, Protein from Triticum aestivum)
40s ribosomal protein es6 (28 kDa, Protein from Triticum aestivum)
60s ribosomal protein el24 (18 kDa, Protein from Triticum aestivum)
40s ribosomal protein us3 (25 kDa, Protein from Triticum aestivum)
60s ribosomal protein ul29 (14 kDa, Protein from Triticum aestivum)
40s ribosomal protein es25 (12 kDa, Protein from Triticum aestivum)
60s ribosomal protein ul23 (16 kDa, Protein from Triticum aestivum)
40s ribosomal protein es30 (6 kDa, Protein from Triticum aestivum)
60s ribosomal protein el33 (12 kDa, Protein from Triticum aestivum)
40s ribosomal protein us10 (14 kDa, Protein from Triticum aestivum)
Rack1 (36 kDa, Protein from Triticum aestivum)
60s ribosomal protein el34 (13 kDa, Protein from Triticum aestivum)
60s ribosomal protein el13 (24 kDa, Protein from Triticum aestivum)
60s ribosomal rna (1 MDa, RNA from Triticum aestivum)
60s ribosomal protein el27 (15 kDa, Protein from Triticum aestivum)
60s ribosomal protein el39 (9 kDa, Protein from Triticum aestivum)
40s ribosomal protein us17 (17 kDa, Protein from Triticum aestivum)
5s ribosomal rna (38 kDa, RNA from Triticum aestivum)
60s ribosomal protein ul6 (21 kDa, Protein from Triticum aestivum)
40s ribosomal protein es4 (30 kDa, Protein from Triticum aestivum)
40s ribosomal protein us7 (22 kDa, Protein from Triticum aestivum)
4-{(2r)-2-[(1s,3s,5s)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione (281 Da, Ligand)
40s ribosomal protein us14 (6 kDa, Protein from Triticum aestivum)
60s ribosomal protein el29 (6 kDa, Protein from Triticum aestivum)
40s ribosomal protein es28 (9 kDa, Protein from Triticum aestivum)
40s ribosomal protein es26 (16 kDa, Protein from Triticum aestivum)
40s ribosomal protein us5 (29 kDa, Protein from Triticum aestivum)
60s ribosomal protein el6 (24 kDa, Protein from Triticum aestivum)
40s ribosomal protein es8 (25 kDa, Protein from Triticum aestivum)
60s ribosomal protein el38 (8 kDa, Protein from Triticum aestivum)
Mrna (2 kDa, RNA from Triticum aestivum)
60s ribosomal protein ul15 (16 kDa, Protein from Triticum aestivum)
60s ribosomal protein el43 (10 kDa, Protein from Triticum aestivum)
40s ribosomal protein us13 (17 kDa, Protein from Triticum aestivum)
40s ribosomal protein us12 (15 kDa, Protein from Triticum aestivum)
40s ribosomal protein es19 (17 kDa, Protein from Triticum aestivum)
Trnai (24 kDa, RNA from Triticum aestivum)
60s ribosomal protein ul14 (15 kDa, Protein from Triticum aestivum)
60s ribosomal protein ul3 (44 kDa, Protein from Triticum aestivum)
40s ribosomal protein es24 (15 kDa, Protein from Triticum aestivum)
Wheat 80s ribosome pausing on aug-stop with cycloheximide (Complex from Triticum aestivum)
60s ribosomal protein el20 (21 kDa, Protein from Triticum aestivum)
60s ribosomal protein ul4 (44 kDa, Protein from Triticum aestivum)
60s ribosomal protein el30 (12 kDa, Protein from Triticum aestivum)
40s ribosomal protein us2 (33 kDa, Protein from Triticum aestivum)
5.8s ribosomal rna (51 kDa, RNA from Triticum aestivum)
40s ribosomal protein us11 (16 kDa, Protein from Triticum aestivum)
60s ribosomal protein ul24 (17 kDa, Protein from Triticum aestivum)
60s ribosomal protein el41 (3 kDa, Protein from Oryza sativa)
40s ribosomal protein us19 (17 kDa, Protein from Triticum aestivum)
40s ribosomal protein es1 (30 kDa, Protein from Triticum aestivum)
60s ribosomal protein el18 (21 kDa, Protein from Triticum aestivum)
Zinc ion (65 Da, Ligand)
40s ribosomal protein us4 (22 kDa, Protein from Triticum aestivum)
40s ribosomal protein us8 (14 kDa, Protein from Triticum aestivum)
60s ribosomal protein ul18 (34 kDa, Protein from Triticum aestivum)
40s ribosomal protein us9 (16 kDa, Protein from Triticum aestivum)
40s ribosomal protein us15 (17 kDa, Protein from Triticum aestivum)
40s ribosomal protein es27 (9 kDa, Protein from Triticum aestivum)
60s ribosomal protein el19 (24 kDa, Protein from Triticum aestivum)
40s ribosomal protein es17 (16 kDa, Protein from Triticum aestivum)
60s ribosomal protein el32 (15 kDa, Protein from Triticum aestivum)
40s ribosomal protein es21 (9 kDa, Protein from Triticum aestivum)
60s ribosomal protein ul2 (28 kDa, Protein from Triticum aestivum)
40s ribosomal protein es10 (20 kDa, Protein from Triticum aestivum)
60s ribosomal protein ul13 (23 kDa, Protein from Triticum aestivum)
60s ribosomal protein el22 (14 kDa, Protein from Triticum aestivum)
Magnesium ion (24 Da, Ligand)
60s ribosomal protein el42 (12 kDa, Protein from Triticum aestivum)
60s ribosomal protein ul16 (24 kDa, Protein from Triticum aestivum)
18s ribosomal rna (583 kDa, RNA from Triticum aestivum)
40s ribosomal protein es7 (22 kDa, Protein from Triticum aestivum)
60s ribosomal protein el37 (10 kDa, Protein from Triticum aestivum)
60s ribosomal protein ul5 (20 kDa, Protein from Triticum aestivum)
60s ribosomal protein el36 (12 kDa, Protein from Triticum aestivum)
40s ribosomal protein el8 (29 kDa, Protein from Triticum aestivum)
60s ribosomal protein el21 (18 kDa, Protein from Triticum aestivum)
60s ribosomal protein el31 (14 kDa, Protein from Triticum aestivum)
60s ribosomal protein el15 (24 kDa, Protein from Triticum aestivum)
60s ribosomal protein el28 (16 kDa, Protein from Triticum aestivum)

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