Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6n7w
Q-score: 0.324
Gao Y, Cui Y, Fox T, Lin S, Wang H, de Val N, Zhou ZH, Yang W
Science (2019) 363 [ Pubmed: 30679383 DOI: doi:10.1126/science.aav7003 ]
- Dna-directed dna polymerase (79 kDa, Protein from Enterobacteria phage T7)
- Dna (25-mer) (7 kDa, DNA from Enterobacteria phage T7)
- Thymidine-5'-triphosphate (482 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna (77-mer) (23 kDa, DNA from Enterobacteria phage T7)
- Trxa (11 kDa, Protein from Escherichia coli)
- D5ae7a mutant gp5 dna polymerase complexed with trx, fork dna, and incoming dttp (from gp4-gp5 leading-strand complexes) (Complex from Enterobacteria phage T7)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6n9u
Q-score: 0.477
Gao Y, Cui Y, Fox T, Lin S, Wang H, de Val N, Zhou ZH, Yang W
Science (2019) 363 [ Pubmed: 30679383 DOI: doi:10.1126/science.aav7003 ]
- Dna primase/helicase (62 kDa, Protein from Enterobacteria phage T7)
- Thymidine-5'-triphosphate (482 Da, Ligand)
- Rna (5'-r(*ap*cp*cp*ap*g)-d(p*(doc))-3') (1 kDa, RNA from Enterobacteria phage T7)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dna-directed dna polymerase (79 kDa, Protein from Enterobacteria phage T7)
- Gp5 dna polymerase and two gp4 primase subunits complexed with trx, rna/dna hybrid, and incoming dttp (from gp4-gp5 lagging-strand complex lags1) (Complex from Enterobacteria phage T7)
- Dna (44-mer) (13 kDa, DNA from Enterobacteria phage T7)
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6n9v
Q-score: 0.408
Gao Y, Cui Y, Fox T, Lin S, Wang H, de Val N, Zhou ZH, Yang W
Science (2019) 363 [ Pubmed: 30679383 DOI: doi:10.1126/science.aav7003 ]
- Gp5 dna polymerase and two gp4 primase subunits complexed with trx, rna/dna hybrid, and incoming dttp (from gp4-gp5 lagging-strand complex lags1) (Complex from Enterobacteria phage T7)
- Dna primase/helicase (62 kDa, Protein from Enterobacteria phage T7)
- Primer (1 kDa, RNA from Enterobacteria phage T7)
- Template (13 kDa, DNA from Enterobacteria phage T7)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Thymidine-5'-triphosphate (482 Da, Ligand)
- Dna-directed dna polymerase (79 kDa, Protein from Enterobacteria phage T7)
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6n9w
Q-score: 0.334
Gao Y, Cui Y, Fox T, Lin S, Wang H, de Val N, Zhou ZH, Yang W
Science (2019) 363 [ Pubmed: 30679383 DOI: doi:10.1126/science.aav7003 ]
- Dna primase/helicase (62 kDa, Protein from Enterobacteria phage T7)
- Thymidine-5'-triphosphate (482 Da, Ligand)
- Template (13 kDa, DNA from Enterobacteria phage T7)
- Primer (1 kDa, RNA from Enterobacteria phage T7)
- Gp5 dna polymerase binding to a and b subunits of gp4 primase-helicase hexamer complexed with trx, rna/dna hybrid, and incoming dttp (lags2) (Complex from Enterobacteria phage T7)
- Magnesium ion (24 Da, Ligand)
- Dna-directed dna polymerase (79 kDa, Protein from Enterobacteria phage T7)
- Zinc ion (65 Da, Ligand)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6n9x
Q-score: 0.371
Gao Y, Cui Y, Fox T, Lin S, Wang H, de Val N, Zhou ZH, Yang W
Science (2019) 363 [ Pubmed: 30679383 DOI: doi:10.1126/science.aav7003 ]
- Dna primase/helicase (62 kDa, Protein from Enterobacteria phage T7)
- Thymidine-5'-triphosphate (482 Da, Ligand)
- Template (13 kDa, DNA from Enterobacteria phage T7)
- Primer (1 kDa, RNA from Enterobacteria phage T7)
- Magnesium ion (24 Da, Ligand)
- Dna-directed dna polymerase (79 kDa, Protein from Enterobacteria phage T7)
- Zinc ion (65 Da, Ligand)
- Gp5 dna polymerase binding to b and c subunits of gp4 primase-helicase hexamer complexed with trx, rna/dna hybrid, and incoming dttp (lags3) (Complex from Enterobacteria phage T7)
Gao Y, Cui Y, Fox T, Lin S, Wang H, de Val N, Zhou ZH, Yang W
Science (2019) 363 [ Pubmed: 30679383 DOI: doi:10.1126/science.aav7003 ]
- Dna (DNA from Enterobacteria phage T7)
- Two t7 dna polymerase/trx complexes interacting with primase domains of t7 helicase (gp4), the first with primase a and b in complex with rna/dna hybrid and dttp, and the second one with only primase c and d (from gp4-gp5 lagging-strand complex lags4) (Complex from Enterobacteria phage T7)
- Gene product 4 helicase-primase (Protein from Enterobacteria phage T7)
- Rna primer (RNA from Enterobacteria phage T7)
- Gene product 5 dna polymerase (Protein from Enterobacteria phage T7)
Gao Y, Cui Y, Fox T, Lin S, Wang H, de Val N, Zhou ZH, Yang W
Science (2019) 363 [ Pubmed: 30679383 DOI: doi:10.1126/science.aav7003 ]
- Primer (DNA from Enterobacteria phage T7)
- Template (DNA from Enterobacteria phage T7)
- Gene product 4 helicase-primase (Protein from Enterobacteria phage T7)
- Thioredoxin (Protein from Escherichia coli)
- T7 dna polymerase/trx interacting with two primase domains (e and f) of t7 helicase (gp4) in complex with a fork dna substrate and dttp (from gp4-gp5 lagging-strand complex lagl1) (Complex from Enterobacteria phage T7)
- Gene product 5 dna polymerase (Protein from Enterobacteria phage T7)
Gao Y, Cui Y, Fox T, Lin S, Wang H, de Val N, Zhou ZH, Yang W
Science (2019) 363 [ Pubmed: 30679383 DOI: doi:10.1126/science.aav7003 ]
- T7 dna polymerase/trx interacting with two primase domains (c and d) of t7 helicase (gp4) in complex with a fork dna substrate and dttp (from gp4-gp5 lagging-strand complex lagl2) (Complex from Enterobacteria phage T7)
- Gene product 4 helicase-primase (Protein from Enterobacteria phage T7)
- Template (DNA from Enterobacteria phage T7)
- Thioredoxin (Protein from Escherichia coli)
- Gene product 5 dna polymerase (Protein from Enterobacteria phage T7)
- Primer (DNA from Enterobacteria phage T7)
Gao Y, Cui Y, Fox T, Lin S, Wang H, de Val N, Zhou ZH, Yang W
Science (2019) 363 [ Pubmed: 30679383 DOI: doi:10.1126/science.aav7003 ]
- Thioredoxin 1 (Protein from Escherichia coli)
- Gene product 5 dna polymerase (Protein from Enterobacteria phage T7)
- Gene product 4 helicase-primase (Protein from Enterobacteria phage T7)
- T7 dna polymerase/trx interacting with two primase domains (b and c) of t7 helicase (gp4) in complex with a fork dna substrate and dttp (from gp4-gp5 lagging-strand complex lagl3) (Complex from Enterobacteria phage T7)
- Template (DNA from Enterobacteria phage T7)
- Primer (DNA from Enterobacteria phage T7)
Gao Y, Cui Y, Fox T, Lin S, Wang H, de Val N, Zhou ZH, Yang W
Science (2019) 363 [ Pubmed: 30679383 DOI: doi:10.1126/science.aav7003 ]
- Thioredoxin 1 (Protein from Escherichia coli)
- T7 dna polymerase/trx interacting with two primase domains (a and b) of t7 helicase (gp4) in complex with a fork dna substrate and dttp (from gp4-gp5 lagging-strand complex lagl4) (Complex from Enterobacteria phage T7)
- Gene product 5 dna polymerase (Protein from Enterobacteria phage T7)
- Gene product 4 helicase-primase (Protein from Enterobacteria phage T7)
- Template (DNA from Enterobacteria phage T7)
- Primer (DNA from Enterobacteria phage T7)