Cryo-EM structure of APOBEC3G-Vif complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8h0i
Q-score: 0.451
Kouno T, Shibata S, Shigematsu M, Hyun J, Kim TG, Matsuo H, Wolf M
Nat Commun (2023) 14 pp. 4037-4037 [ Pubmed: 37419875 DOI: doi:10.1038/s41467-023-39796-5 ]
- Apovec3g (Complex from Human Immunodeficiency virus 1)
- Apobec3g (43 kDa, Protein from Homo sapiens)
- Rna (5'-r(*cp*gp*gp*up*up*gp*ap*up*cp*gp*up*up*up*up*ap*ap*cp*ap*a)-3') (6 kDa, RNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Vif (Complex from Homo sapiens)
- Core binding factor beta (18 kDa, Protein from Homo sapiens)
- Chloride ion (35 Da, Ligand)
- A3g-vc-rna complex (Complex)
- Rna (Complex)
- Viral infectivity factor (18 kDa, Protein from Human immunodeficiency virus 1)
Cryo-EM structure of APOBEC3G-Vif complex
Kouno T, Shibata S, Shigematsu M, Hyun J, Kim TG, Matsuo H, Wolf M
Nat Commun (2023) 14 pp. 4037-4037 [ Pubmed: 37419875 DOI: doi:10.1038/s41467-023-39796-5 ]
Cryo-EM structure of APOBEC3G-Vif complex
Kouno T, Shibata S, Shigematsu M, Hyun J, Kim TG, Matsuo H, Wolf M
Nat Commun (2023) 14 pp. 4037-4037 [ Pubmed: 37419875 DOI: doi:10.1038/s41467-023-39796-5 ]
Cryo-EM structure of APOBEC3G-Vif complex
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 8j62
Q-score: 0.554
Kouno T, Shibata S, Shigematsu M, Hyun J, Kim TG, Matsuo H, Wolf M
Nat Commun (2023) 14 pp. 4037-4037 [ Pubmed: 37419875 DOI: doi:10.1038/s41467-023-39796-5 ]
- Apobec3g,core binding factor (Complex from Homo sapiens)
- Apobec3g (43 kDa, Protein from Homo sapiens)
- Viral infectibity factor (Complex from Human immunodeficiency virus 1)
- Zinc ion (65 Da, Ligand)
- Viral infectivity factor (18 kDa, Protein from Human immunodeficiency virus 1)
- Core binding factor beta (18 kDa, Protein from Homo sapiens)
- Rna (5'-r(*cp*gp*gp*up*up*gp*ap*up*up*gp*up*up*up*up*ap*ap*cp*ap*a)-3') (6 kDa, RNA from synthetic construct)
- A3g-vc-rna complex (Complex)
- Rna (Complex)
Cryo-EM structure of human APOBEC3G/HIV-1 Vif/CBFbeta/ELOB/ELOC monomeric complex
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8cx0
Q-score: 0.509
Li YL, Langley CA, Azumaya CM, Echeverria I, Chesarino NM, Emerman M, Cheng Y, Gross JD
Nature (2023) 615 pp. 728-733 [ DOI: doi:10.1038/s41586-023-05779-1 Pubmed: 36754086 ]
- Elongin-c (12 kDa, Protein from Homo sapiens)
- Rna (5'-r(p*ap*ap*ap*ap*ap*ap*ap*a)-3') (2 kDa, RNA from Spodoptera frugiperda)
- Elongin-b (13 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Hiv-1 vif-e3 ligase substrate receptor (vcbc) in complex with human apobec3g and rna (Complex from Homo sapiens)
- Virion infectivity factor (22 kDa, Protein from Human immunodeficiency virus 1)
- Dna dc->du-editing enzyme apobec-3g (50 kDa, Protein from Homo sapiens)
- Core-binding factor subunit beta (21 kDa, Protein from Homo sapiens)
Cryo-EM structure of human APOBEC3G/HIV-1 Vif/CBFbeta/ELOB/ELOC dimeric complex in State 1
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8cx1
Q-score: 0.366
Li YL, Langley CA, Azumaya CM, Echeverria I, Chesarino NM, Emerman M, Cheng Y, Gross JD
Nature (2023) 615 pp. 728-733 [ DOI: doi:10.1038/s41586-023-05779-1 Pubmed: 36754086 ]
- Rna (5'-r(p*up*ap*ap*ap*up*ap*up*u)-3') (2 kDa, RNA from Spodoptera frugiperda)
- Elongin-c (12 kDa, Protein from Homo sapiens)
- Rna (5'-r(p*ap*ap*ap*ap*up*ap*up*u)-3') (2 kDa, RNA from Spodoptera frugiperda)
- Elongin-b (13 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Hiv-1 vif-e3 ligase substrate receptor (vcbc) in complex with human apobec3g and rna (Complex from Homo sapiens)
- Virion infectivity factor (22 kDa, Protein from Human immunodeficiency virus 1)
- Dna dc->du-editing enzyme apobec-3g (50 kDa, Protein from Homo sapiens)
- Core-binding factor subunit beta (21 kDa, Protein from Homo sapiens)
Cryo-EM structure of human APOBEC3G/HIV-1 Vif/CBFbeta/ELOB/ELOC dimeric complex in State 2
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8cx2
Q-score: 0.375
Li YL, Langley CA, Azumaya CM, Echeverria I, Chesarino NM, Emerman M, Cheng Y, Gross JD
Nature (2023) 615 pp. 728-733 [ DOI: doi:10.1038/s41586-023-05779-1 Pubmed: 36754086 ]
- Elongin-b (13 kDa, Protein from Homo sapiens)
- Elongin-c (12 kDa, Protein from Homo sapiens)
- Rna (5'-r(p*ap*ap*up*ap*ap*ap*ap*ap*u)-3') (2 kDa, RNA from Spodoptera frugiperda)
- Dna dc->du-editing enzyme apobec-3g (50 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Rna (5'-r(p*up*up*up*ap*ap*ap*ap*ap*u)-3') (2 kDa, RNA from Spodoptera frugiperda)
- Core-binding factor subunit beta (21 kDa, Protein from Homo sapiens)
- Hiv-1 vif-e3 ligase substrate receptor (vcbc) in complex with human apobec3g and rna (Complex from Homo sapiens)
- Virion infectivity factor (22 kDa, Protein from Human immunodeficiency virus 1)
Cryo-EM map of human APOBEC3G/HIV-1 Vif/CBFbeta/ELOB/ELOC dimeric complex in State 1-prime
Li YL, Langley CA, Azumaya CM, Echeverria I, Chesarino NM, Emerman M, Cheng Y, Gross JD
Nature (2023) 615 pp. 728-733 [ Pubmed: 36754086 DOI: doi:10.1038/s41586-023-05779-1 ]
Full-length APOBEC3G in complex with HIV-1 Vif, CBF-beta, and fork RNA
Resolution: 3.57 Å
EM Method: Single-particle
Fitted PDBs: 8e40
Q-score: 0.343
Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS
Sci Adv (2023) 9 pp. eade3168-eade3168 [ Pubmed: 36598981 DOI: doi:10.1126/sciadv.ade3168 ]
- Complex of apobec3g with hiv-1 vif and cbf-beta bound to fork rna (99 kDa, Complex from Human immunodeficiency virus 1)
- Virion infectivity factor (21 kDa, Protein from Human immunodeficiency virus 1)
- Core-binding factor subunit beta (18 kDa, Protein from Homo sapiens)
- Rna (6 kDa, RNA from Escherichia coli)
- Rna (8 kDa, RNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna dc->du-editing enzyme apobec-3g (45 kDa, Protein from Macaca mulatta)
APOBEC3G in complex with HIV-1 Vif/CBF-beta/EloB/EloC/Cul5/Rbx2
Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS
Sci Adv (2023) 9 pp. eade3168-eade3168 [ Pubmed: 36598981 DOI: doi:10.1126/sciadv.ade3168 ]
- Elongin-b (Protein from Homo sapiens)
- Elongin-c (Protein from Homo sapiens)
- Cullin-5 (Protein from Homo sapiens)
- Ring-box protein 2 (Protein from Homo sapiens)
- Virion infectivity factor (Protein from Human immunodeficiency virus 1)
- Complex of apobec3g with hiv-1 vif, cbf-beta, elongin-b, elongin-c, cullin-5, and ring-box protein 2 (210 kDa, Complex from Human immunodeficiency virus 1)
- Apobec3g (Protein from Macaca mulatta)
- Core-binding factor subunit beta (Protein from Homo sapiens)