Composite structure of Dynein-Dynactin-JIP3-LIS1
Resolution: 10.0 Å
EM Method: Single-particle
Fitted PDBs: 8ptk
Q-score: 0.202
Singh K, Lau CK, Manigrasso G, Gama JB, Gassmann R, Carter AP
Science (2024) 383 pp. eadk8544-eadk8544 [ DOI: doi:10.1126/science.adk8544 Pubmed: 38547289 ]
- Arp1 actin related protein 1 homolog a (42 kDa, Protein from Sus scrofa)
- Platelet-activating factor acetylhydrolase ib subunit beta (46 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Arp11 (46 kDa, Protein from Sus scrofa)
- Dynactin subunits (Complex from Sus scrofa)
- F-actin-capping protein subunit beta (30 kDa, Protein from Sus scrofa)
- Dynein light chain tctex-type 1 (12 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- C-jun-amino-terminal kinase-interacting protein 3 (65 kDa, Protein from Homo sapiens)
- Cytoplasmic dynein 1 heavy chain 1 (533 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Dynein and jip3 (Complex from Homo sapiens)
- Dynein light chain roadblock-type 1 (10 kDa, Protein from Homo sapiens)
- Dynein light chain 1, cytoplasmic (10 kDa, Protein from Homo sapiens)
- Cytoplasmic dynein 1 intermediate chain 2 (68 kDa, Protein from Homo sapiens)
- Cytoplasmic dynein 1 light intermediate chain 2 (54 kDa, Protein from Homo sapiens)
- Dynactin subunit 2 (44 kDa, Protein from Sus scrofa)
- Capping protein (actin filament) muscle z-line, alpha 1 (33 kDa, Protein from Sus scrofa)
- Dynactin subunit 4 (52 kDa, Protein from Sus scrofa)
- Zinc ion (65 Da, Ligand)
- Dynactin 6 (20 kDa, Protein from Sus scrofa)
- Actin, cytoplasmic 1 (41 kDa, Protein from Sus scrofa)
- Dynactin subunit 3 (21 kDa, Protein from Sus scrofa)
- Dynactin subunit 1 (142 kDa, Protein from Sus scrofa)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Dynactin subunit 5 (20 kDa, Protein from Sus scrofa)
- Composite structure of dynein-dynactin-jip3-lis1 (Complex)
The barbed end complex of dynactin bound to BICDR1 and the cytoplasmic dynein tails (A2, B1, B2)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7z8i
Q-score: 0.466
Nature (2022) 610 pp. 212-216 [ Pubmed: 36071160 DOI: doi:10.1038/s41586-022-05186-y ]
- Cytoplasmic dynein 1 intermediate chain 2 (71 kDa, Protein from Homo sapiens)
- Cytoplasmic dynein 1 heavy chain 1 (533 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Bicd family-like cargo adapter 1 (65 kDa, Protein from Mus musculus)
- Arp1 actin related protein 1 homolog a (42 kDa, Protein from pig)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dynein, cytoplasmic 1 (Cellular component from Homo sapiens)
- Bicdr1 (Cellular component from Mus musculus)
- Cytoplasmic dynein 1 light intermediate chain 2 (54 kDa, Protein from Homo sapiens)
- Dynactin (Cellular component from Sus scrofa)
- F-actin capping protein beta subunit (30 kDa, Protein from pig)
- Capping protein (actin filament) muscle z-line, alpha 1 (33 kDa, Protein from pig)
- Complex of dynein, dynactin, and bicdr1 bound to microtubules (130 kDa, Complex)
Composite structure of dynein-dynactin-BICDR on microtubules
Resolution: 20.0 Å
EM Method: Single-particle
Fitted PDBs: 7z8f
Q-score: 0.215
Nature (2022) 610 pp. 212-216 [ Pubmed: 36071160 DOI: doi:10.1038/s41586-022-05186-y ]
- Arp1 actin related protein 1 homolog a (42 kDa, Protein from pig)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Arp11 (46 kDa, Protein from pig)
- Dynactin subunit 5 (20 kDa, Protein from pig)
- Dynactin subunit 1 (142 kDa, Protein from pig)
- Dynactin subunit 3 (21 kDa, Protein from pig)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Cytoplasmic dynein 1 intermediate chain 2 (71 kDa, Protein from Homo sapiens)
- Cytoplasmic dynein 1 heavy chain 1 (533 kDa, Protein from Homo sapiens)
- Bicd family-like cargo adapter 1 (65 kDa, Protein from Mus musculus)
- Magnesium ion (24 Da, Ligand)
- Complex of dynein, dynactin, and bicdr1 bound to microtubules (4 MDa, Complex)
- Dynein light chain roadblock-type 1 (10 kDa, Protein from Homo sapiens)
- Cytoplasmic dynein 1 light intermediate chain 2 (54 kDa, Protein from Homo sapiens)
- Capping protein (actin filament) muscle z-line, alpha 1 (33 kDa, Protein from pig)
- Dynactin 6 (20 kDa, Protein from pig)
- Dynactin subunit 2 (44 kDa, Protein from pig)
- Dynein, cytoplasmic 1 (1 MDa, Cellular component from Homo sapiens)
- Dynactin (1 MDa, Cellular component from Sus scrofa)
- Actin, cytoplasmic 1 (41 kDa, Protein from pig)
- Dynactin subunit 4 (52 kDa, Protein from pig)
- Zinc ion (65 Da, Ligand)
- F-actin capping protein beta subunit (30 kDa, Protein from pig)
- Bicdr1 (130 kDa, Cellular component from Mus musculus)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
Cryo-EM structure of the dynactin complex at 3.8 Angstrom resolution
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6znl
Q-score: 0.352
Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP
EMBO J (2021) 40 pp. e106164-e106164 [ DOI: doi:10.15252/embj.2020106164 Pubmed: 33734450 ]
- Dynactin subunit 5 (20 kDa, Protein from Sus scrofa)
- Dynactin subunit 4 (52 kDa, Protein from Sus scrofa)
- Dynactin subunit 2 (44 kDa, Protein from Sus scrofa)
- Dynactin subunit 3 (21 kDa, Protein from Sus scrofa)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Capping protein (actin filament) muscle z-line, alpha 1 (33 kDa, Protein from Sus scrofa)
- Actin, cytoplasmic 1 (41 kDa, Protein from Sus scrofa)
- Dynactin complex (1 MDa, Complex from Sus scrofa)
- F-actin capping protein beta subunit (30 kDa, Protein from Sus scrofa)
- Dynactin 6 (20 kDa, Protein from Sus scrofa)
- Arp11 (46 kDa, Protein from Sus scrofa)
- Dynactin subunit 1 (142 kDa, Protein from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Arp1 actin related protein 1 homolog a (42 kDa, Protein from Sus scrofa)
Cryo-EM structure of two dynein tail domains bound to dynactin and BICDR1
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6f1t
Q-score: 0.269
Urnavicius L, Lau CK, Elshenawy MM, Morales-Rios E, Motz C, Yildiz A, Carter AP
Nature (2018) 554 pp. 202-206 [ DOI: doi:10.1038/nature25462 Pubmed: 29420470 ]
- Cryo-em map of two dynein tail domains bound to dynactin and bicdr1 (Complex)
- F-actin capping protein beta subunit (30 kDa, Protein from Sus scrofa)
- Dynactin subunit 1 (4 kDa, Protein from Sus scrofa)
- Dynactin subunit 2 (7 kDa, Protein from Sus scrofa)
- Bicd family-like cargo adapter 1,bicd family-like cargo adapter 1,bicd family-like cargo adapter 1 (42 kDa, Protein from Mus musculus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Cytoplasmic dynein 1 light intermediate chain 2 (54 kDa, Protein from Homo sapiens)
- Dynein (Complex from Homo sapiens)
- Dynactin subunit 3 (5 kDa, Protein from Sus scrofa)
- Bicd family-like cargo adapter 1 (Complex from Mus musculus)
- Dynactin subunit 2 (7 kDa, Protein from Sus scrofa)
- Dynactin subunit 2 (52 kDa, Protein from Sus scrofa)
- Cytoplasmic dynein 1 intermediate chain 2 (68 kDa, Protein from Homo sapiens)
- Dynactin (Complex from Sus scrofa)
- Dynactin subunit 2 (2 kDa, Protein from Sus scrofa)
- Arp1 actin related protein 1 homolog a (42 kDa, Protein from Sus scrofa)
- Actin related protein 10 homolog (43 kDa, Protein from Sus scrofa)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Actin, cytoplasmic 1 (41 kDa, Protein from Sus scrofa)
- Dynactin subunit 2 (50 kDa, Protein from Sus scrofa)
- Dynactin 6 (20 kDa, Protein from Sus scrofa)
- Cytoplasmic dynein 1 heavy chain 1,cytoplasmic dynein 1 heavy chain 1,cytoplasmic dynein 1 heavy chain 1,dynein heavy chain,cytoplasmic dynein 1 heavy chain 1,cytoplasmic dynein 1 heavy chain 1,cytoplasmic dynein 1 heavy chain 1,cytoplasmic dynein 1 heavy chain 1,dynein heavy chain,cytoplasmic dynein 1 heavy chain 1,cytoplasmic dynein 1 heavy chain 1 (119 kDa, Protein from Homo sapiens)
- Dynein light chain roadblock-type 1 (10 kDa, Protein from Homo sapiens)
- Dynactin subunit 5 (20 kDa, Protein from Sus scrofa)
- Dynactin subunit 4 (22 kDa, Protein from Sus scrofa)
- Capping protein (actin filament) muscle z-line, alpha 1 (33 kDa, Protein from Sus scrofa)
- Dynactin subunit 2 (10 kDa, Protein from Sus scrofa)
- Bicd family-like cargo adapter 1,bicd family-like cargo adapter 1,bicd family-like cargo adapter 1,bicdr-1 (42 kDa, Protein from Mus musculus)
- Dynactin subunit 1 (4 kDa, Protein from Sus scrofa)
- Dynactin subunit 2 (5 kDa, Protein from Sus scrofa)
N terminal region of dynein tail domains in complex with dynactin filament and BICDR-1
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6f1u
Q-score: 0.374
Urnavicius L, Lau CK, Elshenawy MM, Morales-Rios E, Motz C, Yildiz A, Carter AP
Nature (2018) 554 pp. 202-206 [ DOI: doi:10.1038/nature25462 Pubmed: 29420470 ]
- Capping protein (actin filament) muscle z-line, alpha 1 (33 kDa, Protein from Pig)
- Dynactin subunit 2 (5 kDa, Protein from Pig)
- Cytoplasmic dynein 1 heavy chain 1 (136 kDa, Protein from Homo sapiens)
- Two dynein tail domains bound to dynactin and bicdr1. (Complex)
- Bicd family-like cargo adapter 1 (65 kDa, Protein from Mus musculus)
- F-actin capping protein beta subunit (30 kDa, Protein from Pig)
- Bicdr1 (Complex from Mus musculus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dynactin subunit 2 (2 kDa, Protein from Pig)
- Cytoplasmic dynein 1 intermediate chain 2 (68 kDa, Protein from Homo sapiens)
- Dynactin filament (Complex from Sus scrofa)
- Cytoplasmic dynein (Complex from Homo sapiens)
- Arp1 actin related protein 1 homolog a (42 kDa, Protein from Pig)
Cryo-EM structure of two dynein tail domains bound to dynactin and HOOK3
Resolution: 6.7 Å
EM Method: Single-particle
Fitted PDBs: 6f38
Q-score: 0.23
Urnavicius L, Lau CK, Elshenawy MM, Morales-Rios E, Motz C, Yildiz A, Carter AP
Nature (2018) 554 pp. 202-206 [ DOI: doi:10.1038/nature25462 Pubmed: 29420470 ]
- Hook3 (18 kDa, Protein from Homo sapiens)
- Dynactin subunit 2 (3 kDa, Protein from Sus scrofa)
- F-actin capping protein beta subunit (30 kDa, Protein from Sus scrofa)
- Hook3 (Complex from Homo sapiens)
- Dynactin subunit 1 (4 kDa, Protein from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Cytoplasmic dynein 1 light intermediate chain 2 (54 kDa, Protein from Homo sapiens)
- Dynein (Complex from Homo sapiens)
- Dynactin subunit 3 (5 kDa, Protein from Sus scrofa)
- Dynactin subunit 2 (7 kDa, Protein from Sus scrofa)
- Cytoplasmic dynein 1 intermediate chain 2 (68 kDa, Protein from Homo sapiens)
- Dynactin (Complex from Sus scrofa)
- Arp1 actin related protein 1 homolog a (42 kDa, Protein from Sus scrofa)
- Actin related protein 10 homolog (43 kDa, Protein from Sus scrofa)
- Dynactin subunit 2 (5 kDa, Protein from Sus scrofa)
- Dynactin subunit 2 (7 kDa, Protein from Sus scrofa)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Actin, cytoplasmic 1 (41 kDa, Protein from Sus scrofa)
- Dynactin 6 (20 kDa, Protein from Sus scrofa)
- Cytoplasmic dynein 1 heavy chain 1 (168 kDa, Protein from Homo sapiens)
- Dynactin subunit 2 (10 kDa, Protein from Sus scrofa)
- Dynein light chain roadblock-type 1 (10 kDa, Protein from Homo sapiens)
- Dynactin subunit 2 (52 kDa, Protein from Sus scrofa)
- Complex of two dynein tail domains bound to dynactin and hook3 (Complex)
- Dynactin subunit 2 (49 kDa, Protein from Sus scrofa)
- Dynactin subunit 5 (20 kDa, Protein from Sus scrofa)
- Capping protein (actin filament) muscle z-line, alpha 1 (33 kDa, Protein from Sus scrofa)
- Dynactin subunit 1 (4 kDa, Protein from Sus scrofa)
- Dynactin subunit 4 (22 kDa, Protein from Sus scrofa)
Human cytoplasmic dynein-1 bound to dynactin and an N-terminal construct of BICD2
Resolution: 8.7 Å
EM Method: Single-particle
Fitted PDBs: 5nw4
Q-score: 0.113
Zhang K, Foster HE, Rondelet A, Lacey SE, Bahi-Buisson N, Bird AW, Carter AP
Cell (2017) 169 pp. 1303-1314.e18 [ Pubmed: 28602352 DOI: doi:10.1016/j.cell.2017.05.025 ]
- Dynein heavy chain (75 kDa, Protein from Homo sapiens)
- Dynactin 6 (20 kDa, Protein from Pig)
- Dynein intermediate chain (29 kDa, Protein from Homo sapiens)
- Dynactin (Complex from Sus scrofa)
- Dynactin (7 kDa, Protein from Pig)
- Arp11 (46 kDa, Protein from Pig)
- Dynactin (2 kDa, Protein from Pig)
- Capping protein (actin filament) muscle z-line, alpha 1 (33 kDa, Protein from Pig)
- Robl (10 kDa, Protein from Homo sapiens)
- Dynein heavy chain (78 kDa, Protein from Homo sapiens)
- Dynactin shoulder complex (14 kDa, Protein from Pig)
- Arp1 (42 kDa, Protein from Pig)
- Dynein intermediate chain (29 kDa, Protein from Homo sapiens)
- P150 (4 kDa, Protein from Pig)
- Bicd2n (23 kDa, Protein from Homo sapiens)
- Dynein-1 (Complex from Homo sapiens)
- Dynactin (3 kDa, Protein from Pig)
- Dynactin shoulder complex (48 kDa, Protein from Pig)
- Dynactin subunit 5 (24 kDa, Protein from Pig)
- Dynein n-terminal dimerization domain (10 kDa, Protein from Homo sapiens)
- Dynactin shoulder complex (51 kDa, Protein from Pig)
- Dynein n-terminal dimerization domain (10 kDa, Protein from Homo sapiens)
- Dynactin pointed end p62 (20 kDa, Protein from Pig)
- Human cytoplasmic dynein-1 bound to dynactin and an n-terminal construct of bicd2 (2 MDa, Complex)
- Dynactin shoulder complex (11 kDa, Protein from Pig)
- Bicd2 (Complex from Homo sapiens)
- Beta-actin (43 kDa, Protein from Pig)
- Dynein light intermediate chain (25 kDa, Protein from Homo sapiens)
- Dynein light intermediate chain (25 kDa, Protein from Homo sapiens)
- F-actin capping protein beta subunit variant ii (33 kDa, Protein from Pig)
- Dynactin p150 (4 kDa, Protein from Pig)
- Dynactin (5 kDa, Protein from Pig)