Electron Microscopy Data Bank

EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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sample_name:"Cognate target RNA (46-mer)"

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EMD-10117

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Cryo-EM structure of the Type III-B Cmr-beta bound to cognate target RNA and AMPPnP, state 1

Release date: July 8, 2020
Resolution: 2.41 Å
EM Method: Single-particle
Fitted PDBs: 6s8b
Q-score: 0.48

Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G

Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]

Sample:

Cmr1 (55 kDa, Protein from Sulfolobus islandicus REY15A)
Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus (strain REY15A))
Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus (strain REY15A))
Crispr-associated protein cmrx (19 kDa, Protein from Sulfolobus islandicus (strain REY15A))
Manganese (ii) ion (54 Da, Ligand)
Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus (strain REY15A))
Crrna (16 kDa, RNA from Sulfolobus islandicus REY15A)
Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus (strain REY15A))
Cognate target rna (46-mer) (14 kDa, RNA from Sulfolobus islandicus REY15A)
Type iii-b cmr-beta ternary complex, cognate target rna and amppnp bound (900 kDa, Complex from Sulfolobus islandicus REY15A)
Zinc ion (65 Da, Ligand)
Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus (strain REY15A))

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