Electron Microscopy Data Bank

EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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sample_name:"D5AE7A mutant gp5 DNA polymerase complexed with trx, fork DNA, and incoming dTTP (from gp4-gp5 leading-strand complexes)"

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EMD-0365

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Structure of bacteriophage T7 leading-strand DNA polymerase (D5A/E7A)/Trx in complex with a DNA fork and incoming dTTP (from multiple lead complexes)

Release date: March 6, 2019
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6n7w
Q-score: 0.324

Gao Y, Cui Y, Fox T, Lin S, Wang H, de Val N, Zhou ZH, Yang W

Science (2019) 363 [ Pubmed: 30679383 DOI: doi:10.1126/science.aav7003 ]

Sample:

Dna-directed dna polymerase (79 kDa, Protein from Enterobacteria phage T7)
Dna (25-mer) (7 kDa, DNA from Enterobacteria phage T7)
Thymidine-5'-triphosphate (482 Da, Ligand)
Magnesium ion (24 Da, Ligand)
Dna (77-mer) (23 kDa, DNA from Enterobacteria phage T7)
Trxa (11 kDa, Protein from Escherichia coli)
D5ae7a mutant gp5 dna polymerase complexed with trx, fork dna, and incoming dttp (from gp4-gp5 leading-strand complexes) (Complex from Enterobacteria phage T7)

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