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EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Research:

perform basic research in molecular biology

Services:

offer vital services to scientists in the member states

Training

scientists, students and visitors at all levels

Transfer

and development of technology

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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sample_name:"DNA-directed RNA polymerase III subunit RPC6" AND author_name:"Hagen WJH" AND software_ft:"RELION" AND resolution:[4 TO 5}

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EMD-4181

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RNA Polymerase III initially transcribing complex

Release date: Jan. 17, 2018
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6f41
Q-score: 0.342

Vorlander MK, Khatter H, Wetzel R, Hagen WJH, Muller CW

Nature (2018) 553 pp. 295-300 [ Pubmed: 29345638 DOI: doi:10.1038/nature25440 ]

Sample:

Dna-directed rna polymerase iii subunit rpc5 (32 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc1 (162 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Non-template dna (24 kDa, DNA from Saccharomyces cerevisiae S288c)
Dna-directed rna polymerase iii subunit rpc3 (74 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc7 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Rna (1 kDa, RNA from Saccharomyces cerevisiae S288c)
Transcription factors (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc8 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc2 (129 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Template-dna (25 kDa, DNA from Saccharomyces cerevisiae S288c)
Transcription factor iiib 70 kda subunit (67 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Tata-box-binding protein (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc10 (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Rna polymerase iii initially transcribing complex (893 kDa, Complex)
Zinc ion (65 Da, Ligand)
Dna-directed rna polymerase iii subunit rpc9 (18 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc4 (46 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Nucleic acids (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Transcription factor tfiiib component b'' (67 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i and iii subunit rpac2 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc6 (36 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i and iii subunit rpac1 (37 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Rna polymerase iii (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))

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EMD-4183

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RNA Polymerase III closed complex CC2

Release date: Jan. 17, 2018
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 6f44
Q-score: 0.303

Vorlander MK, Khatter H, Wetzel R, Hagen WJH, Muller CW

Nature (2018) 553 pp. 295-300 [ DOI: doi:10.1038/nature25440 Pubmed: 29345638 ]

Sample:

Rna polymerase iii closed complex cc2 (893 kDa, Complex)
Dna-directed rna polymerase iii subunit rpc2 (129 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc10 (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc3 (74 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Non-template dna (24 kDa, DNA from Saccharomyces cerevisiae)
Nucleic acids (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc9 (18 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc8 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Zinc ion (65 Da, Ligand)
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Transcription factor tfiiib component b'' (67 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Template dna (25 kDa, DNA from Saccharomyces cerevisiae)
Transcription factor iiib 70 kda subunit (67 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Rna polymerase iii (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc5 (32 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc1 (162 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Transcription factor iiib (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i and iii subunit rpac1 (37 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i and iii subunit rpac2 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Tata-box-binding protein (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc4 (46 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc6 (36 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc7 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))

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EMDB [2]

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