Human spliceosomal PM5 C* complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8c6j
Q-score: 0.353
Dybkov O, Preussner M, El Ayoubi L, Feng VY, Harnisch C, Merz K, Leupold P, Yudichev P, Agafonov DE, Will CL, Girard C, Dienemann C, Urlaub H, Kastner B, Heyd F, Luehrmann R
Sci Adv (2023) 9 pp. eadf1785- [ DOI: doi:10.1126/sciadv.adf1785 Pubmed: 36867703 ]
- Peptidyl-prolyl cis-trans isomerase-like 3 (18 kDa, Protein from human)
- U5 snrna (36 kDa, RNA from human)
- Crooked neck-like protein 1 (83 kDa, Protein from human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from human)
- Rna-binding protein 8a (19 kDa, Protein from human)
- Pre-mrna-splicing factor isy1 homolog (33 kDa, Protein from human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Intron-binding protein aquarius (171 kDa, Protein from human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from human)
- Protein mago nashi homolog (17 kDa, Protein from human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from human)
- Inositol hexakisphosphate (660 Da, Ligand)
- U6 snrna (34 kDa, RNA from human)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from human)
- Nitric oxide synthase-interacting protein (33 kDa, Protein from human)
- Pm5 5' exon (78 kDa, RNA from Homo sapiens)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from human)
- Pleiotropic regulator 1 (57 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from human)
- U2 snrna (60 kDa, RNA from human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from human)
- Potassium ion (39 Da, Ligand)
- Pre-mrna-splicing factor cwc22 homolog (57 kDa, Protein from human)
- Zinc ion (65 Da, Ligand)
- Atp-dependent rna helicase dhx8 (139 kDa, Protein from human)
- Splicing factor cactin (88 kDa, Protein from human)
- Splicing regulator sde2 (49 kDa, Protein from human)
- Pre-mrna-splicing factor slu7 (68 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from human)
- Cell division cycle 5-like protein (92 kDa, Protein from human)
- Prkr-interacting protein 1 (21 kDa, Protein from human)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from human)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Human spliceosomal c* complex assembled on pm5 pre-mrna (Complex from Homo sapiens)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from human)
- Sting er exit protein (25 kDa, Protein from human)
- Protein fam32a (13 kDa, Protein from human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from human)
- Probable atp-dependent rna helicase ddx41 (69 kDa, Protein from human)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from human)
- Snw domain-containing protein 1 (61 kDa, Protein from human)
- Protein bud31 homolog (17 kDa, Protein from human)
- Nf-kappa-b-activating protein (47 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from human)
- Eukaryotic initiation factor 4a-iii (46 kDa, Protein from human)
- Splicing factor c9orf78 (33 kDa, Protein from human)
- Protein fam50a (40 kDa, Protein from human)
- Pm5 intron (74 kDa, RNA from Homo sapiens)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from human)
- Splicing factor ess-2 homolog (52 kDa, Protein from human)
Structure of an ALYREF-exon junction complex hexamer
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7znj
Q-score: 0.608
Pacheco-Fiallos B, Vorlander MK, Riabov-Bassat D, Fin L, O'Reilly FJ, Ayala FI, Schellhaas U, Rappsilber J, Plaschka C
Nature (2023) 616 pp. 828-835 [ DOI: doi:10.1038/s41586-023-05904-0 Pubmed: 37020021 ]
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Maltose/maltodextrin-binding periplasmic protein,tho complex subunit 4 (57 kDa, Protein from Homo sapiens)
- Rna-binding protein 8a (10 kDa, Protein from Homo sapiens)
- Rna (1 kDa, RNA from Homo sapiens)
- Hexameric complex between the trex subunit alyref and the exon junction complex (650 kDa, Complex from Homo sapiens)
- Protein mago nashi homolog (17 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Eukaryotic initiation factor 4a-iii, n-terminally processed (43 kDa, Protein from Homo sapiens)
The cryo-EM structure of human pre-C*-I complex
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7w59
Q-score: 0.268
Zhan X, Lu Y, Zhang X, Yan C, Shi Y
Mol Cell (2022) 82 pp. 2769-2778.e4 [ DOI: doi:10.1016/j.molcel.2022.05.021 Pubmed: 35705093 ]
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from human)
- Atp-dependent rna helicase dhx8 (139 kDa, Protein from human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from human)
- Cell division cycle 5-like protein (92 kDa, Protein from human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from human)
- U6 snrna (34 kDa, RNA from Human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from human)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from human)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- Crooked neck-like protein 1 (100 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from human)
- Psme3-interacting protein (28 kDa, Protein from human)
- Protein mago nashi homolog (17 kDa, Protein from human)
- Rna-binding protein 8a (19 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from human)
- Human pre-c*-i complex (Complex from Homo sapiens)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from human)
- Snw domain-containing protein 1 (61 kDa, Protein from human)
- Pre-mrna (55 kDa, RNA from unidentified adenovirus)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from human)
- Rna helicase aquarius (171 kDa, Protein from human)
- Eukaryotic initiation factor 4a-iii (46 kDa, Protein from human)
- U5 snrna (37 kDa, RNA from human)
- Pre-mrna (14 kDa, RNA from unidentified adenovirus)
- Zinc ion (65 Da, Ligand)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from human)
- U2 snrna (60 kDa, RNA from human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from human)
- Pleiotropic regulator 1 (57 kDa, Protein from human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from human)
- Protein bud31 homolog (17 kDa, Protein from human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from human)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from human)
- Protein casc3 (76 kDa, Protein from human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from human)
The cryo-EM structure of human pre-C*-II complex
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7w5a
Q-score: 0.269
Zhan X, Lu Y, Zhang X, Yan C, Shi Y
Mol Cell (2022) 82 pp. 2769-2778.e4 [ DOI: doi:10.1016/j.molcel.2022.05.021 Pubmed: 35705093 ]
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from human)
- Atp-dependent rna helicase dhx8 (139 kDa, Protein from human)
- Pre-mrna-splicing factor slu7 (68 kDa, Protein from human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from human)
- Cactin (88 kDa, Protein from human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from human)
- Cell division cycle 5-like protein (92 kDa, Protein from human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from human)
- Pre-mrna (55 kDa, RNA from unidentified adenovirus)
- Human pre-c*-ii complex (Complex from Homo sapiens)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from human)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- Crooked neck-like protein 1 (100 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from human)
- Psme3-interacting protein (28 kDa, Protein from human)
- Protein mago nashi homolog (17 kDa, Protein from human)
- Rna-binding protein 8a (19 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from human)
- Snw domain-containing protein 1 (61 kDa, Protein from human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from human)
- Rna helicase aquarius (171 kDa, Protein from human)
- Eukaryotic initiation factor 4a-iii (46 kDa, Protein from human)
- U5 snrna (37 kDa, RNA from human)
- U6 snrna (34 kDa, RNA from human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from human)
- U2 snrna (60 kDa, RNA from human)
- Zinc ion (65 Da, Ligand)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from human)
- Pleiotropic regulator 1 (57 kDa, Protein from human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from human)
- Prkr-interacting protein 1 (21 kDa, Protein from human)
- Pre-mrna (14 kDa, RNA from unidentified adenovirus)
- Protein bud31 homolog (17 kDa, Protein from human)
- Protein fam32a (13 kDa, Protein from human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from human)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from human)
- Protein casc3 (76 kDa, Protein from human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from human)
The cryo-EM structure of human C* complex
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 7w5b
Q-score: 0.192
Zhan X, Lu Y, Zhang X, Yan C, Shi Y
Mol Cell (2022) 82 pp. 2769-2778.e4 [ DOI: doi:10.1016/j.molcel.2022.05.021 Pubmed: 35705093 ]
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from human)
- Pre-mrna (14 kDa, RNA from unidentified adenovirus)
- Crooked neck-like protein 1 (100 kDa, Protein from human)
- Cactin (88 kDa, Protein from human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from human)
- Rna-binding protein 8a (19 kDa, Protein from human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-processing factor 19 (55 kDa, Protein from human)
- Protein mago nashi homolog (17 kDa, Protein from human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from human)
- Inositol hexakisphosphate (660 Da, Ligand)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from human)
- Pleiotropic regulator 1 (57 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from human)
- U2 snrna (60 kDa, RNA from human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from human)
- U6 snrna (34 kDa, RNA from human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from human)
- Zinc ion (65 Da, Ligand)
- Protein casc3 (76 kDa, Protein from human)
- Atp-dependent rna helicase dhx8 (139 kDa, Protein from human)
- Pre-mrna-splicing factor slu7 (68 kDa, Protein from human)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from human)
- Cell division cycle 5-like protein (92 kDa, Protein from human)
- Rna helicase aquarius (171 kDa, Protein from human)
- Prkr-interacting protein 1 (21 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from human)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from human)
- Protein fam32a (13 kDa, Protein from human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from human)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from human)
- Snw domain-containing protein 1 (61 kDa, Protein from human)
- Protein bud31 homolog (17 kDa, Protein from human)
- Nf-kappa-b-activating protein (47 kDa, Protein from human)
- Human pre-c*-i complex (Complex from Homo sapiens)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from human)
- Eukaryotic initiation factor 4a-iii (46 kDa, Protein from human)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from human)
- Pre-mrna (55 kDa, RNA from unidentified adenovirus)
- U5 snrna (37 kDa, RNA from human)
Cryo-EM structure of a human post-catalytic spliceosome (P complex) at 3.0 angstrom
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6icz
Q-score: 0.284
Zhang X, Zhan X, Yan C, Zhang W, Liu D, Lei J, Shi Y
Cell Res (2019) 29 pp. 274-285 [ DOI: doi:10.1038/s41422-019-0143-x Pubmed: 30728453 ]
- U2snrna (60 kDa, RNA from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Human)
- Human post_catalytic spliceosome (Complex from Homo sapiens)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Human)
- Rna helicase aquarius (171 kDa, Protein from Human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- Atp-dependent rna helicase dhx8 (139 kDa, Protein from Human)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- U6snrna (34 kDa, RNA from Human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- Rna-binding protein 8a (19 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
- Protein bud31 homolog (17 kDa, Protein from Human)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from Human)
- Pre-mrna-splicing factor slu7 (68 kDa, Protein from Human)
- Small nuclear ribonucleoprotein-associated protein (23 kDa, Protein from Human)
- Inositol hexakisphosphate (660 Da, Ligand)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
- Prkr-interacting protein 1 (21 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Zinc ion (65 Da, Ligand)
- Protein mago nashi homolog 2 (17 kDa, Protein from Human)
- Snw domain-containing protein 1 (61 kDa, Protein from Human)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from Human)
- Protein casc3 (76 kDa, Protein from Human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Pre-mrna (87 kDa, RNA from Human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- U5snrna (37 kDa, RNA from Human)
- Eukaryotic initiation factor 4a-iii (46 kDa, Protein from Human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
Human post-catalytic spliceosome (P complex) stalled with DHX8 K594A mutant, overall map
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6qdv
Q-score: 0.278
Fica SM, Oubridge C, Wilkinson ME, Newman AJ, Nagai K
Science (2019) 363 pp. 710-714 [ Pubmed: 30705154 DOI: doi:10.1126/science.aaw5569 ]
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Homo sapiens)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Homo sapiens)
- Atp-dependent rna helicase dhx8 (Complex from Homo sapiens)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein e (9 kDa, Protein from Homo sapiens)
- Protein fam32a (7 kDa, Protein from Homo sapiens)
- Ligated exons: minx mrna (4 kDa, RNA from Human adenovirus 2)
- Snw domain-containing protein 1 (33 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Intron lariat: minx rna (27 kDa, RNA from Human adenovirus 2)
- Pre-mrna-processing factor 17 (59 kDa, Protein from Homo sapiens)
- Pleiotropic regulator 1 (35 kDa, Protein from Homo sapiens)
- Inositol hexakisphosphate (660 Da, Ligand)
- Pre-mrna-processing factor 19 (55 kDa, Protein from Homo sapiens)
- Replication stress response regulator sde2 (4 kDa, Protein from Homo sapiens)
- Atp-dependent rna helicase dhx8 (139 kDa, Protein from Homo sapiens)
- Serine/arginine repetitive matrix protein 2 (2 kDa, Protein from Homo sapiens)
- Potassium ion (39 Da, Ligand)
- Pre-mrna-splicing factor slu7 (68 kDa, Protein from Homo sapiens)
- 116 kda u5 small nuclear ribonucleoprotein component (101 kDa, Protein from Homo sapiens)
- Crooked neck-like protein 1 (100 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein-associated proteins b and b' (9 kDa, Protein from Homo sapiens)
- U6 snrna (34 kDa, RNA from Homo sapiens)
- Pre-mrna-splicing factor syf2 (17 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Pre-mrna-splicing factor rbm22 (33 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Prkr-interacting protein 1 (14 kDa, Protein from Homo sapiens)
- Cell division cycle 5-like protein (92 kDa, Protein from Homo sapiens)
- Eukaryotic initiation factor 4a-iii (44 kDa, Protein from Homo sapiens)
- Cactin (15 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d3 (9 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- U2 snrna (60 kDa, RNA from Homo sapiens)
- Human post-catalytic p complex spliceosome (Complex from Homo sapiens)
- Intron-binding protein aquarius (171 kDa, Protein from Homo sapiens)
- U5 small nuclear ribonucleoprotein 40 kda protein (34 kDa, Protein from Homo sapiens)
- U2 small nuclear ribonucleoprotein b'' (10 kDa, Protein from Homo sapiens)
- Nf-kappa-b-activating protein (3 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein f (8 kDa, Protein from Homo sapiens)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Homo sapiens)
- U5 small nuclear ribonucleoprotein 200 kda helicase (197 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d1 (9 kDa, Protein from Homo sapiens)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Homo sapiens)
- U5 snrna (36 kDa, RNA from Homo sapiens)
- Human post-catalytic p complex spliceosome (3 MDa, Complex)
- Rna-binding protein 8a (10 kDa, Protein from Homo sapiens)
- Protein bud31 homolog (17 kDa, Protein from Homo sapiens)
- Minx introns and exons (Complex from Human adenovirus 2)
- U2 small nuclear ribonucleoprotein a' (18 kDa, Protein from Homo sapiens)
- Protein mago nashi homolog 2 (16 kDa, Protein from Homo sapiens)
The Cryo-EM Structure of Human Catalytic Step I Spliceosome (C complex) at 4.1 angstrom resolution
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 5yzg
Q-score: 0.205
Zhan X, Yan C, Zhang X, Lei J, Shi Y
Science (2018) 359 pp. 537-545 [ DOI: doi:10.1126/science.aar6401 Pubmed: 29301961 ]
- U2 snrna (60 kDa, RNA from Homo sapiens)
- Inositol hexakisphosphate (660 Da, Ligand)
- Rna-binding protein 8a (19 kDa, Protein from Human)
- Snw domain-containing protein 1 (61 kDa, Protein from Human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
- Coiled-coil domain-containing protein 94 (37 kDa, Protein from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (23 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
- Pre-mrna-splicing factor cwc25 homolog (49 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Intron-binding protein aquarius (171 kDa, Protein from Human)
- Peptidylprolyl isomerase domain and wd repeat-containing protein 1 (73 kDa, Protein from Human)
- Pre-mrna-splicing factor atp-dependent rna helicase prp16 (140 kDa, Protein from Human)
- Zinc ion (65 Da, Ligand)
- Unknow (2 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- Protein casc3 (76 kDa, Protein from Human)
- Pre-mrna-splicing factor isy1 homolog (35 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Human)
- Protein bud31 homolog (17 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- Pre-mrna (88 kDa, RNA from Human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- U6 snrna (34 kDa, RNA from Homo sapiens)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from Human)
- Eukaryotic initiation factor 4a-iii (46 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase g (88 kDa, Protein from Human)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from Human)
- U5 snrna (37 kDa, RNA from Homo sapiens)
- Protein mago nashi homolog 2 (17 kDa, Protein from Human)
- Human catalytic step i spliceosome (Complex from Homo sapiens)
Cryo-EM structure of the human spliceosome just prior to exon ligation at 3.6 angstrom
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 5xjc
Q-score: 0.22
Zhang X, Yan C, Hang J, Finci LI, Lei J, Shi Y
Cell (2017) 169 pp. 918-929.e14 [ DOI: doi:10.1016/j.cell.2017.04.033 Pubmed: 28502770 ]
- Prkr-interacting protein 1 (21 kDa, Protein from Human)
- Atp-dependent rna helicase dhx8 (139 kDa, Protein from Human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Rna-binding protein 8a (19 kDa, Protein from Human)
- Homo sapiens small nuclear rna (u2) gene (60 kDa, RNA from Homo sapiens)
- Snw domain-containing protein 1 (61 kDa, Protein from Human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
- Pre-mrna (87 kDa, RNA from Human adenovirus 2)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (23 kDa, Protein from Human)
- Intron-binding protein aquarius (171 kDa, Protein from Human)
- Zinc ion (65 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- Protein casc3 (76 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Human)
- Pre-mrna-splicing factor slu7 (68 kDa, Protein from Human)
- Protein bud31 homolog (17 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- U6 snrna (34 kDa, RNA from Homo sapiens)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from Human)
- Eukaryotic initiation factor 4a-iii (46 kDa, Protein from Human)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from Human)
- Human c* spliceosome (Complex from Homo sapiens)
- U5 snrna (37 kDa, RNA from Homo sapiens)
- Protein mago nashi homolog 2 (17 kDa, Protein from Human)
Cryo-EM structure of a human spliceosome activated for step 2 of splicing (C* complex)
Resolution: 5.9 Å
EM Method: Single-particle
Fitted PDBs: 5mqf
Q-score: 0.202
Bertram K, Agafonov DE, Liu WT, Dybkov O, Will CL, Hartmuth K, Urlaub H, Kastner B, Stark H, Lu Hrmann R
Nature (2017) 542 pp. 318-323 [ DOI: doi:10.1038/nature21079 Pubmed: 28076346 ]
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
- Intron-binding protein aquarius (173 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
- Homo sapiens u5 a small nuclear rna (36 kDa, RNA from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- Human c* spliceosome, masked refinement (Complex from Homo sapiens)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Human gene for small nuclear rna u2 (snrna u2) (60 kDa, RNA from Human)
- Minx pre-mrna (intron) (104 kDa, RNA from Human)
- Atp-dependent rna helicase dhx8 (139 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from Human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- Snw domain-containing protein 1 (61 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- Protein bud31 homolog (17 kDa, Protein from Human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- Homo sapiens rna, u6 small nuclear 1 (rnu6-1), small nuclear rna (34 kDa, RNA from Human)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from Human)
- Eukaryotic initiation factor 4a-iii (46 kDa, Protein from Human)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from Human)