Cryo-EM structure of an orphan GPCR-Gi protein signaling complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8g05
Q-score: 0.524
Zhang X, Wang Y, Supekar S, Cao X, Zhou J, Dang J, Chen S, Jenkins L, Marsango S, Li X, Liu G, Milligan G, Feng M, Fan H, Gong W, Zhang C
Nat Commun (2023) 14 pp. 5706-5706 [ DOI: doi:10.1038/s41467-023-41201-0 Pubmed: 37709767 ]
- Cholesterol (386 Da, Ligand)
- 6-(octylamino)pyrimidine-2,4(3h,5h)-dione (239 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- 6oau_gpr84_dngi_scfv16 (Complex from Spodoptera frugiperda)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 84 (48 kDa, Protein from Homo sapiens)
Cryo-EM structure of the 3-OH-C12-bound GPR84 receptor-Gi complex
Resolution: 2.89 Å
EM Method: Single-particle
Fitted PDBs: 8j18
Q-score: 0.499
Liu H, Zhang Q, He X, Jiang M, Wang S, Yan X, Cheng X, Liu Y, Nan FJ, Xu HE, Xie X, Yin W
Nat Commun (2023) 14 pp. 3271-3271 [ DOI: doi:10.1038/s41467-023-38985-6 Pubmed: 37277332 ]
- G-protein coupled receptor 84 (45 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (28 kDa, Protein from synthetic construct)
- (3r)-3-hydroxydodecanoic acid (216 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- 3-oh-c12-bound gpr84 receptor-gi complex (Complex from Homo sapiens)
Cryo-EM structure of the LY237-bound GPR84 receptor-Gi complex
Resolution: 3.23 Å
EM Method: Single-particle
Fitted PDBs: 8j19
Q-score: 0.49
Liu H, Zhang Q, He X, Jiang M, Wang S, Yan X, Cheng X, Liu Y, Nan FJ, Xu HE, Xie X, Yin W
Nat Commun (2023) 14 pp. 3271-3271 [ Pubmed: 37277332 DOI: doi:10.1038/s41467-023-38985-6 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (28 kDa, Protein from synthetic construct)
- 6-nonylpyridine-2,4-diol (237 Da, Ligand)
- Ly237-bound gpr84 receptor-gi complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 84 (45 kDa, Protein from Homo sapiens)
Cryo-EM structure of the GPR84 receptor-Gi complex with no ligand modeled
Resolution: 3.24 Å
EM Method: Single-particle
Fitted PDBs: 8j1a
Q-score: 0.484
Liu H, Zhang Q, He X, Jiang M, Wang S, Yan X, Cheng X, Liu Y, Nan FJ, Xu HE, Xie X, Yin W
Nat Commun (2023) 14 pp. 3271-3271 [ DOI: doi:10.1038/s41467-023-38985-6 Pubmed: 37277332 ]
- G-protein coupled receptor 84 (45 kDa, Protein from Homo sapiens)
- Gpr84 receptor-gi complex with no ligand modeled (Complex from Homo sapiens)
- Antibody fragment scfv16 (28 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)