Electron Microscopy Data Bank

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Structure of bacterial 30S-IF1-IF3-mRNA translation pre-initiation complex (state-1B)

Release date: Oct. 5, 2016
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 5lmo
Q-score: 0.281

Hussain T, Llacer JL, Wimberly BT, Kieft JS, Ramakrishnan V

Cell (2016) 167 pp. 133-144.e13 [ DOI: doi:10.1016/j.cell.2016.08.074 Pubmed: 27662086 ]

Sample:

30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
16s rrna (493 kDa, RNA from Thermus thermophilus HB8)
30s ribosomal protein s17 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein s5 (17 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein s20 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein s2 (29 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein s7 (18 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein s11 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Guanosine-5'-monophosphate (363 Da, Ligand)
30s ribosomal protein s13 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein s19 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Zinc ion (65 Da, Ligand)
Translation initiation factor if-1 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein s16 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein s18 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein thx (3 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Magnesium ion (24 Da, Ligand)
30s-if1-if3-mrna pre-initiation complex (state-1b) (826 kDa, Complex)
30s ribosomal protein s12 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Uridine-5'-monophosphate (324 Da, Ligand)
30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein s3 (26 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Mrna (12 kDa, RNA from Thermus thermophilus)
Translation initiation factor if-3 (19 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Adenosine-5'-monophosphate (347 Da, Ligand)

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