Cryo-EM structure of the yeast B*-a2 complex at an average resolution of 3.2 angstrom
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6j6g
Q-score: 0.317
Wan R, Bai R, Yan C, Lei J, Shi Y
Cell (2019) 177 pp. 339-351.e13 [ Pubmed: 30879786 DOI: doi:10.1016/j.cell.2019.02.006 ]
- Act1 pre-mrna (217 kDa, RNA from Baker's yeast)
- Pre-mrna-splicing factor slt11 (40 kDa, Protein from Baker's yeast)
- U2 snrna (376 kDa, RNA from Baker's yeast)
- U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor snt309 (20 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
- Pre-mrna-processing protein 45 (42 kDa, Protein from Baker's yeast)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor clf1 (82 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor syf2 (24 kDa, Protein from Baker's yeast)
- Magnesium ion (24 Da, Ligand)
- Pre-mrna-splicing factor snu114 (114 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cef1 (67 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc22 (67 kDa, Protein from Baker's yeast)
- Inositol hexakisphosphate (660 Da, Ligand)
- U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor prp46 (50 kDa, Protein from Baker's yeast)
- Pre-mrna-processing factor 19 (56 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
- Zinc ion (65 Da, Ligand)
- Pre-mrna-splicing factor bud31 (18 kDa, Protein from Baker's yeast)
- U6 snrna (35 kDa, RNA from Baker's yeast)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Baker's yeast)
- Catalytically activated spliceosome b*-a2 complex (Complex from Saccharomyces cerevisiae S288c)
- U5 snrna (68 kDa, RNA from Baker's yeast)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
- Pre-mrna-processing factor 17 (52 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor isy1 (28 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor 8 (279 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Baker's yeast)
Cryo-EM structure of the yeast B*-a1 complex at an average resolution of 3.6 angstrom
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6j6h
Q-score: 0.283
Wan R, Bai R, Yan C, Lei J, Shi Y
Cell (2019) 177 pp. 339-351.e13 [ Pubmed: 30879786 DOI: doi:10.1016/j.cell.2019.02.006 ]
- Pre-mrna-splicing factor slt11 (40 kDa, Protein from Baker's yeast)
- U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae S288c)
- U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor snt309 (20 kDa, Protein from Baker's yeast)
- Pre-mrna-processing protein 45 (42 kDa, Protein from Baker's yeast)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor clf1 (82 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor syf2 (24 kDa, Protein from Baker's yeast)
- U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae S288c)
- Magnesium ion (24 Da, Ligand)
- Act1 pre-mrna (217 kDa, RNA from Saccharomyces cerevisiae S288c)
- Catalytically activated spliceosome b*-a1 complex (Complex from Saccharomyces cerevisiae S288C)
- Pre-mrna-splicing factor snu114 (114 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cef1 (67 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc22 (67 kDa, Protein from Baker's yeast)
- Inositol hexakisphosphate (660 Da, Ligand)
- U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor prp46 (50 kDa, Protein from Baker's yeast)
- Pre-mrna-processing factor 19 (56 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
- Zinc ion (65 Da, Ligand)
- Pre-mrna-splicing factor bud31 (18 kDa, Protein from Baker's yeast)
- U6 snrna (35 kDa, RNA from Baker's yeast)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
- Pre-mrna-processing factor 17 (52 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor isy1 (28 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor 8 (279 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Baker's yeast)
Cryo-EM structure of the yeast B*-b2 complex at an average resolution of 3.7 angstrom
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6j6q
Q-score: 0.261
Wan R, Bai R, Yan C, Lei J, Shi Y
Cell (2019) 177 pp. 339-351.e13 [ DOI: doi:10.1016/j.cell.2019.02.006 Pubmed: 30879786 ]
- Inositol hexakisphosphate (660 Da, Ligand)
- Catalytically activated spliceosome b*-a2 complex (Complex from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor cwc22 (67 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Baker's yeast)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor bud31 (18 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor clf1 (82 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-processing protein 45 (42 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
- Zinc ion (65 Da, Ligand)
- Pre-mrna-splicing factor isy1 (28 kDa, Protein from Baker's yeast)
- U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae S288c)
- U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-processing factor 17 (52 kDa, Protein from Baker's yeast)
- U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Baker's yeast)
- U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor 8 (279 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cef1 (67 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor prp46 (50 kDa, Protein from Baker's yeast)
- Ubc4 pre-mrna (78 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor snu114 (114 kDa, Protein from Baker's yeast)
- Magnesium ion (24 Da, Ligand)
- Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Baker's yeast)
- U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
- Splicing factor yju2 (32 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor syf2 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor slt11 (40 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor snt309 (20 kDa, Protein from Baker's yeast)
- Pre-mrna-processing factor 19 (56 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
Cryo-EM structure of a human post-catalytic spliceosome (P complex) at 3.0 angstrom
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6icz
Q-score: 0.284
Zhang X, Zhan X, Yan C, Zhang W, Liu D, Lei J, Shi Y
Cell Res (2019) 29 pp. 274-285 [ DOI: doi:10.1038/s41422-019-0143-x Pubmed: 30728453 ]
- U2snrna (60 kDa, RNA from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Human)
- Human post_catalytic spliceosome (Complex from Homo sapiens)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Human)
- Rna helicase aquarius (171 kDa, Protein from Human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- Atp-dependent rna helicase dhx8 (139 kDa, Protein from Human)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- U6snrna (34 kDa, RNA from Human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- Rna-binding protein 8a (19 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
- Protein bud31 homolog (17 kDa, Protein from Human)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from Human)
- Pre-mrna-splicing factor slu7 (68 kDa, Protein from Human)
- Small nuclear ribonucleoprotein-associated protein (23 kDa, Protein from Human)
- Inositol hexakisphosphate (660 Da, Ligand)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
- Prkr-interacting protein 1 (21 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Zinc ion (65 Da, Ligand)
- Protein mago nashi homolog 2 (17 kDa, Protein from Human)
- Snw domain-containing protein 1 (61 kDa, Protein from Human)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from Human)
- Protein casc3 (76 kDa, Protein from Human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Pre-mrna (87 kDa, RNA from Human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- U5snrna (37 kDa, RNA from Human)
- Eukaryotic initiation factor 4a-iii (46 kDa, Protein from Human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6id0
Q-score: 0.314
Zhang X, Zhan X, Yan C, Zhang W, Liu D, Lei J, Shi Y
Cell Res (2019) 29 pp. 274-285 [ DOI: doi:10.1038/s41422-019-0143-x Pubmed: 30728453 ]
- U2snrna (60 kDa, RNA from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Human)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Human)
- Rna helicase aquarius (171 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- U6snrna (34 kDa, RNA from Human)
- Human intron lariat spliceosome (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Protein bud31 homolog (17 kDa, Protein from Human)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from Human)
- Small nuclear ribonucleoprotein-associated protein (23 kDa, Protein from Human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Cwf19-like protein 2 (103 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Zinc ion (65 Da, Ligand)
- Snw domain-containing protein 1 (61 kDa, Protein from Human)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from Human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- U5snrna (37 kDa, RNA from Human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from Human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- Pre-mrna (87 kDa, RNA from Human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
Resolution: 2.86 Å
EM Method: Single-particle
Fitted PDBs: 6id1
Q-score: 0.291
Zhang X, Zhan X, Yan C, Zhang W, Liu D, Lei J, Shi Y
Cell Res (2019) 29 pp. 274-285 [ Pubmed: 30728453 DOI: doi:10.1038/s41422-019-0143-x ]
- Inositol hexakisphosphate (660 Da, Ligand)
- Snw domain-containing protein 1 (61 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- U6snrna (34 kDa, RNA from Human)
- Rna helicase aquarius (171 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Pre-mrna-splicing factor atp-dependent rna helicase dhx15 (91 kDa, Protein from Human)
- Zinc ion (65 Da, Ligand)
- Small nuclear ribonucleoprotein-associated protein (23 kDa, Protein from Human)
- Pre-mrna (87 kDa, RNA from Human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- Protein bud31 homolog (17 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
- Cwf19-like protein 2 (103 kDa, Protein from Human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- Human intron lariat spliceosome (Complex from Homo sapiens)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from Human)
- U2snrna (60 kDa, RNA from Human)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from Human)
- U5snrna (37 kDa, RNA from Human)
Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 5zwo
Q-score: 0.191
Bai R, Wan R, Yan C, Lei J, Shi Y
Science (2018) 360 pp. 1423-1429 [ Pubmed: 29794219 DOI: doi:10.1126/science.aau0325 ]
- U4/u6 small nuclear ribonucleoprotein prp4 (52 kDa, Protein from Saccharomyces cerevisiae S288c)
- U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor snu114 (114 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna leakage protein 1 (23 kDa, Protein from Saccharomyces cerevisiae S288c)
- 23 kda u4/u6.u5 small nuclear ribonucleoprotein component (22 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor spp381 (33 kDa, Protein from Saccharomyces cerevisiae S288c)
- U6 snrna-associated sm-like protein lsm8 (12 kDa, Protein from Saccharomyces cerevisiae S288c)
- U6 snrna-associated sm-like protein lsm4 (21 kDa, Protein from Saccharomyces cerevisiae S288c)
- 66 kda u4/u6.u5 small nuclear ribonucleoprotein component (66 kDa, Protein from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Saccharomyces cerevisiae S288c)
- U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor 38 (27 kDa, Protein from Saccharomyces cerevisiae S288c)
- Cold sensitive u2 snrna suppressor 1 (50 kDa, Protein from Saccharomyces cerevisiae S288c)
- Spliceosomal protein dib1 (16 kDa, Protein from Saccharomyces cerevisiae S288c)
- U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Saccharomyces cerevisiae S288c)
- U6 snrna-associated sm-like protein lsm3 (10 kDa, Protein from Saccharomyces cerevisiae S288c)
- U4 snrna (51 kDa, RNA from Saccharomyces cerevisiae S288c)
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor prp21 (33 kDa, Protein from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Saccharomyces cerevisiae S288c)
- U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae S288c)
- U2 snrnp component hsh155 (110 kDa, Protein from Saccharomyces cerevisiae S288c)
- U2 snrnp component ist3 (17 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor 6 (104 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor prp11 (29 kDa, Protein from Saccharomyces cerevisiae S288c)
- U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor 8 (279 kDa, Protein from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna (19 kDa, RNA from Saccharomyces cerevisiae S288c)
- Protein hsh49 (24 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor prp9 (63 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing helicase brr2 (246 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-processing factor 31 (56 kDa, Protein from Saccharomyces cerevisiae S288c)
- U6 snrna-associated sm-like protein lsm5 (10 kDa, Protein from Saccharomyces cerevisiae S288c)
- U4/u6 small nuclear ribonucleoprotein prp3 (55 kDa, Protein from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor rds3 (12 kDa, Protein from Saccharomyces cerevisiae S288c)
- Rds3 complex subunit 10 (10 kDa, Protein from Saccharomyces cerevisiae S288c)
- U6 snrna-associated sm-like protein lsm6 (9 kDa, Protein from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Saccharomyces cerevisiae S288c)
- Yeast fully assembled spliceosomal b complex before activation (Cell from Saccharomyces cerevisiae S288c)
- U6 snrna-associated sm-like protein lsm2 (11 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor cwc26 (30 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor rse1 (153 kDa, Protein from Saccharomyces cerevisiae S288c)
- U6 snrna-associated sm-like protein lsm7 (13 kDa, Protein from Saccharomyces cerevisiae S288c)
- 13 kda ribonucleoprotein-associated protein (13 kDa, Protein from Saccharomyces cerevisiae S288c)
Resolution: 3.46 Å
EM Method: Single-particle
Fitted PDBs: 5y88
Q-score: 0.264
Wan R, Yan C, Bai R, Lei J, Shi Y
Cell (2017) 171 pp. 120-132 [ DOI: doi:10.1016/j.cell.2017.08.029 Pubmed: 28919079 ]
- U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor cwc23 (33 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Intron lariat (10 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-processing factor 19 (56 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-splicing factor ntr2 (36 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-splicing factor snu114 (114 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor spp382 (83 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Inositol hexakisphosphate (660 Da, Ligand)
- S. cerevisiae intron lariat spliceosome (ils) (Complex from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-splicing factor clf1 (82 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-splicing factor bud31 (18 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Zinc ion (65 Da, Ligand)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor 8 (279 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-splicing factor prp46 (50 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-processing factor 17 (52 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Protein cwc16 (32 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Magnesium ion (24 Da, Ligand)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor syf2 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-splicing factor slt11 (40 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-splicing factor cef1 (67 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-splicing factor snt309 (20 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-processing protein 45 (42 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-splicing factor atp-dependent rna helicase prp43 (87 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Rna (intron or u6 snrna) (2 kDa, RNA from Saccharomyces cerevisiae S288c)
Cryo-EM Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae at 3.6 angstrom
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 5ylz
Q-score: 0.251
Bai R, Yan C, Wan R, Lei J, Shi Y
Cell (2017) 171 pp. 1589-1598.e8 [ DOI: doi:10.1016/j.cell.2017.10.038 Pubmed: 29153833 ]
- Pre-mrna-splicing factor 18 (28 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor slu7 (44 kDa, Protein from Saccharomyces cerevisiae S288c)
- U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor snu114 (114 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor prp46 (50 kDa, Protein from Saccharomyces cerevisiae S288c)
- Mrna/intron lariat (113 kDa, RNA from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor cef1 (67 kDa, Protein from Saccharomyces cerevisiae S288c)
- U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Saccharomyces cerevisiae S288c)
- U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor clf1 (82 kDa, Protein from Saccharomyces cerevisiae S288c)
- Inositol hexakisphosphate (660 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-processing factor 17 (52 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor snt309 (20 kDa, Protein from Saccharomyces cerevisiae S288c)
- The post-catalytic spliceosome (p complex) (Complex from Saccharomyces cerevisiae S288c)
- Pre-mrna-processing protein 45 (42 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor cwc22 (67 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor 8 (279 kDa, Protein from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Saccharomyces cerevisiae S288c)
- U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Saccharomyces cerevisiae S288c)
- Zinc ion (65 Da, Ligand)
- Pre-mrna-processing factor 19 (56 kDa, Protein from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor slt11 (40 kDa, Protein from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor syf2 (24 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor bud31 (18 kDa, Protein from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor atp-dependent rna helicase prp22 (130 kDa, Protein from Saccharomyces cerevisiae S288c)
- U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Saccharomyces cerevisiae S288c)