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EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Research:

perform basic research in molecular biology

Services:

offer vital services to scientists in the member states

Training

scientists, students and visitors at all levels

Transfer

and development of technology

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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sample_name:"GUANOSINE-5'-TRIPHOSPHATE" AND author_orcid:"0000-0001-9186-0361" AND citation_journal_name:"SCIENCE" AND microscope_name:"FEI TITAN KRIOS" AND author_name:"Fica SM"

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EMD-4525

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Human post-catalytic spliceosome (P complex) stalled with DHX8 K594A mutant, overall map

Release date: Feb. 20, 2019
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6qdv
Q-score: 0.278

Fica SM, Oubridge C, Wilkinson ME, Newman AJ, Nagai K

Science (2019) 363 pp. 710-714 [ Pubmed: 30705154 DOI: doi:10.1126/science.aaw5569 ]

Sample:

Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Homo sapiens)
Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Homo sapiens)
Atp-dependent rna helicase dhx8 (Complex from Homo sapiens)
Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Homo sapiens)
Pre-mrna-splicing factor syf1 (100 kDa, Protein from Homo sapiens)
Small nuclear ribonucleoprotein e (9 kDa, Protein from Homo sapiens)
Protein fam32a (7 kDa, Protein from Homo sapiens)
Ligated exons: minx mrna (4 kDa, RNA from Human adenovirus 2)
Snw domain-containing protein 1 (33 kDa, Protein from Homo sapiens)
Pre-mrna-splicing factor spf27 (26 kDa, Protein from Homo sapiens)
Guanosine-5'-triphosphate (523 Da, Ligand)
Intron lariat: minx rna (27 kDa, RNA from Human adenovirus 2)
Pre-mrna-processing factor 17 (59 kDa, Protein from Homo sapiens)
Pleiotropic regulator 1 (35 kDa, Protein from Homo sapiens)
Inositol hexakisphosphate (660 Da, Ligand)
Pre-mrna-processing factor 19 (55 kDa, Protein from Homo sapiens)
Replication stress response regulator sde2 (4 kDa, Protein from Homo sapiens)
Atp-dependent rna helicase dhx8 (139 kDa, Protein from Homo sapiens)
Serine/arginine repetitive matrix protein 2 (2 kDa, Protein from Homo sapiens)
Potassium ion (39 Da, Ligand)
Pre-mrna-splicing factor slu7 (68 kDa, Protein from Homo sapiens)
116 kda u5 small nuclear ribonucleoprotein component (101 kDa, Protein from Homo sapiens)
Crooked neck-like protein 1 (100 kDa, Protein from Homo sapiens)
Small nuclear ribonucleoprotein-associated proteins b and b' (9 kDa, Protein from Homo sapiens)
U6 snrna (34 kDa, RNA from Homo sapiens)
Pre-mrna-splicing factor syf2 (17 kDa, Protein from Homo sapiens)
Zinc ion (65 Da, Ligand)
Pre-mrna-splicing factor rbm22 (33 kDa, Protein from Homo sapiens)
Adenosine-5'-triphosphate (507 Da, Ligand)
Prkr-interacting protein 1 (14 kDa, Protein from Homo sapiens)
Cell division cycle 5-like protein (92 kDa, Protein from Homo sapiens)
Eukaryotic initiation factor 4a-iii (44 kDa, Protein from Homo sapiens)
Cactin (15 kDa, Protein from Homo sapiens)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Homo sapiens)
Small nuclear ribonucleoprotein sm d3 (9 kDa, Protein from Homo sapiens)
Magnesium ion (24 Da, Ligand)
U2 snrna (60 kDa, RNA from Homo sapiens)
Human post-catalytic p complex spliceosome (Complex from Homo sapiens)
Intron-binding protein aquarius (171 kDa, Protein from Homo sapiens)
U5 small nuclear ribonucleoprotein 40 kda protein (34 kDa, Protein from Homo sapiens)
U2 small nuclear ribonucleoprotein b'' (10 kDa, Protein from Homo sapiens)
Nf-kappa-b-activating protein (3 kDa, Protein from Homo sapiens)
Small nuclear ribonucleoprotein f (8 kDa, Protein from Homo sapiens)
Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Homo sapiens)
U5 small nuclear ribonucleoprotein 200 kda helicase (197 kDa, Protein from Homo sapiens)
Small nuclear ribonucleoprotein sm d1 (9 kDa, Protein from Homo sapiens)
Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Homo sapiens)
U5 snrna (36 kDa, RNA from Homo sapiens)
Human post-catalytic p complex spliceosome (3 MDa, Complex)
Rna-binding protein 8a (10 kDa, Protein from Homo sapiens)
Protein bud31 homolog (17 kDa, Protein from Homo sapiens)
Minx introns and exons (Complex from Human adenovirus 2)
U2 small nuclear ribonucleoprotein a' (18 kDa, Protein from Homo sapiens)
Protein mago nashi homolog 2 (16 kDa, Protein from Homo sapiens)

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EMD-3979

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Post-catalytic P complex spliceosome with 3' splice site docked

Release date: Jan. 17, 2018
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6exn
Q-score: 0.296

Wilkinson ME, Fica SM, Galej WP, Norman CM, Newman AJ, Nagai K

Science (2017) 358 pp. 1283-1288 [ DOI: doi:10.1126/science.aar3729 Pubmed: 29146871 ]

Sample:

Pre-mrna-splicing factor atp-dependent rna helicase prp22 (130 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor slt11 (40 kDa, Protein from Baker's yeast)
U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor snt309 (20 kDa, Protein from Baker's yeast)
Pre-mrna-processing protein 45 (42 kDa, Protein from Baker's yeast)
Guanosine-5'-triphosphate (523 Da, Ligand)
Intron lariat: ubc4 rna (30 kDa, RNA from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor syf2 (24 kDa, Protein from Baker's yeast)
U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae S288c)
Magnesium ion (24 Da, Ligand)
Pre-mrna-splicing factor snu114 (114 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc22 (67 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc16 (32 kDa, Protein from Baker's yeast)
Ligated exons: ubc4 mrna (12 kDa, RNA from Saccharomyces cerevisiae S288c)
3' exon (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-mrna-splicing factor prp8 (279 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor slu7 (44 kDa, Protein from Baker's yeast)
Inositol hexakisphosphate (660 Da, Ligand)
Pre-mrna-splicing factor prp18 (28 kDa, Protein from Baker's yeast)
U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor clf1 (82 kDa, Protein from Baker's yeast)
P complex spliceosome (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-mrna-splicing factor cef1 (67 kDa, Protein from Baker's yeast)
Pre-mrna-processing factor prp19 (56 kDa, Protein from Baker's yeast)
P complex spliceosome with 3' exon docked (2 MDa, Complex)
Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor prp46 (50 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
Zinc ion (65 Da, Ligand)
Pre-mrna-splicing factor syf1 (100 kDa, Protein from Baker's yeast)
Unassigned structure (8 kDa, Protein from Baker's yeast)
U6 snrna (35 kDa, RNA from Baker's yeast)
Pre-mrna-splicing factor bud31 (18 kDa, Protein from Baker's yeast)
U5 snrna (57 kDa, RNA from Baker's yeast)
Pre-mrna-processing factor prp17 (52 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Baker's yeast)

[Show more]

EMD-3539

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Structure of a spliceosome remodeled for exon ligation

Release date: Jan. 18, 2017
Resolution: 3.85 Å
EM Method: Single-particle
Fitted PDBs: 5mps
Q-score: 0.356

Fica SM, Oubridge C, Galej WP, Wilkinson ME, Bai XC, Newman AJ, Nagai K

Nature (2017) 542 pp. 377-380 [ Pubmed: 28076345 DOI: doi:10.1038/nature21078 ]

Sample:

Pre-mrna-splicing factor cef1 (67 kDa, Protein from Baker's yeast)
Potassium ion (39 Da, Ligand)
Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor slu7 (44 kDa, Protein from Baker's yeast)
Ubc4 gene exon (6 kDa, RNA from Saccharomyces cerevisiae)
Pre-mrna-splicing factor snu114 (114 kDa, Protein from Baker's yeast)
Guanosine-5'-triphosphate (523 Da, Ligand)
Pre-mrna-splicing factor clf1 (82 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor bud31 (18 kDa, Protein from Baker's yeast)
Unknown (5 kDa, Protein from Baker's yeast)
Magnesium ion (24 Da, Ligand)
Pre-mrna-splicing factor syf1 (101 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor slt11 (40 kDa, Protein from Baker's yeast)
Saccharomyces cerevisiae strain t.52_2h chromosome xii sequence (35 kDa, RNA from Saccharomyces cerevisiae)
Inositol hexakisphosphate (660 Da, Ligand)
Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor 8 (279 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor prp46 (50 kDa, Protein from Baker's yeast)
Yeast ubc4 gene for ubiquitin-conjugating enzyme (30 kDa, RNA from Saccharomyces cerevisiae)
Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
U5 snrna (57 kDa, RNA from Saccharomyces cerevisiae)
Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
Zinc ion (65 Da, Ligand)
Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor syf2 (24 kDa, Protein from Baker's yeast)
U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae)
Pre-mrna-processing protein 45 (42 kDa, Protein from Baker's yeast)
Saccharomyces cerevisiae spliceosome. complex c just after prp16-mediated remodeling (Complex)
Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc22 (67 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor 18 (28 kDa, Protein from Baker's yeast)
Pre-mrna-processing factor 17 (52 kDa, Protein from Baker's yeast)

[Show more]

EMD-3541

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Structure of a spliceosome remodeled for exon ligation

Release date: Jan. 18, 2017
Resolution: 4.17 Å
EM Method: Single-particle
Fitted PDBs: 5mq0
Q-score: 0.223

Fica SM, Oubridge C, Galej WP, Wilkinson ME, Bai XC, Newman AJ, Nagai K

Nature (2017) 542 pp. 377-380 [ Pubmed: 28076345 DOI: doi:10.1038/nature21078 ]

Sample:

Pre-mrna-splicing factor cef1 (67 kDa, Protein from Baker's yeast)
Potassium ion (39 Da, Ligand)
Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor snt309 (20 kDa, Protein from Baker's yeast)
3'-exon of ubc4 pre-mrna, bound by prp22 helicase (873 Da, RNA from Saccharomyces cerevisiae)
Pre-mrna-splicing factor slu7 (44 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor snu114 (114 kDa, Protein from Baker's yeast)
Guanosine-5'-triphosphate (523 Da, Ligand)
S.cerevisiae chromosome ii reading frame orf ybr230c (376 kDa, RNA from Baker's yeast)
Pre-mrna-splicing factor clf1 (82 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor bud31 (18 kDa, Protein from Baker's yeast)
Magnesium ion (24 Da, Ligand)
Pre-mrna-splicing factor slt11 (40 kDa, Protein from Baker's yeast)
Unknown protein (5 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor syf1,pre-mrna-splicing factor syf1 (101 kDa, Protein from Baker's yeast)
U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Baker's yeast)
Inositol hexakisphosphate (660 Da, Ligand)
Pre-mrna-processing factor 19 (56 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Baker's yeast)
Saccharomyces cerevisiae strain t.52_2h chromosome xii sequence (35 kDa, RNA from Baker's yeast)
Pre-mrna-splicing factor 8 (279 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor prp46 (50 kDa, Protein from Baker's yeast)
Yeast ubc4 gene for ubiquitin-conjugating enzyme (30 kDa, RNA from Saccharomyces cerevisiae)
Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor atp-dependent rna helicase prp22 (130 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
Zinc ion (65 Da, Ligand)
Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor syf2 (24 kDa, Protein from Baker's yeast)
Saccharomyces cerevisiae strain wi_c_mbsp_4 chromosome vii sequence (57 kDa, RNA from Baker's yeast)
Pre-mrna-processing protein 45 (42 kDa, Protein from Baker's yeast)
U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Baker's yeast)
Saccharomyces cerevisiae spliceosome. complex c just after prp16-mediated remodeling (Complex)
Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc22 (67 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
5'-exon of ubc4 pre-mrna (6 kDa, RNA from Saccharomyces cerevisiae)
Pre-mrna-splicing factor 18 (28 kDa, Protein from Baker's yeast)
Pre-mrna-processing factor 17 (52 kDa, Protein from Baker's yeast)

[Show more]

EMD-3542

Download Map 3D View Raw data

Structure of a spliceosome remodeled for exon ligation

Release date: Jan. 18, 2017
Resolution: 4.6 Å
EM Method: Single-particle

Fica SM, Galej WP, Wilkinson ME, Oubridge C, Bai X-C, Newman AJ, Nagai K

Nature (2017) 542 pp. 377-380 [ DOI: doi:10.1038/nature21078 Pubmed: 28076345 ]

Sample:

Saccharomyces cerevisiae spliceosome. complex c just after prp16-mediated remodeling (Complex)
Inositol hexakisphosphate (660 Da, Ligand)
Intron of ubc4 pre-mrna (30 kDa, RNA from Saccharomyces cerevisiae)
Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor snu114 (114 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor slt11 (40 kDa, Protein from Baker's yeast)
U2 snrna (376 kDa, RNA from Baker's yeast)
Pre-mrna-splicing factor syf2 (24 kDa, Protein from Baker's yeast)
Guanosine-5'-triphosphate (523 Da, Ligand)
Pre-mrna-splicing factor bud31 (18 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor syf1 (100 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cef1 (67 kDa, Protein from Baker's yeast)
U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor prp46 (50 kDa, Protein from Baker's yeast)
Potassium ion (39 Da, Ligand)
Zinc ion (65 Da, Ligand)
Unknown protein (5 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor clf1 (82 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor slu7 (44 kDa, Protein from Baker's yeast)
Pre-mrna-processing protein 45 (42 kDa, Protein from Baker's yeast)
3'exon of ubc4 pre-mrna bound to prp22 helicase (873 Da, RNA from Saccharomyces cerevisiae)
5'exon of ubc4 pre-mrna (6 kDa, RNA from Saccharomyces cerevisiae)
Pre-mrna-processing factor 17 (52 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor atp-dependent rna helicase prp22 (130 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc22 (67 kDa, Protein from Baker's yeast)
Magnesium ion (24 Da, Ligand)
U6 snrna (35 kDa, RNA from Baker's yeast)
U5 snrna (57 kDa, RNA from Baker's yeast)
Pre-mrna-splicing factor 8 (279 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor 18 (28 kDa, Protein from Baker's yeast)

[Show more]

Sort by: Download

Showing 1 - 5 of 5. Page 1 of 1

Display: Statistics

Download

Select database:

EMDB [5]

Format:

Select the script type:

Shell (Unix-based) Batch (Windows-based)

Preview first 10

Field: