Human post-catalytic spliceosome (P complex) stalled with DHX8 K594A mutant, overall map
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6qdv
Q-score: 0.278
Fica SM, Oubridge C, Wilkinson ME, Newman AJ, Nagai K
Science (2019) 363 pp. 710-714 [ Pubmed: 30705154 DOI: doi:10.1126/science.aaw5569 ]
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Homo sapiens)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Homo sapiens)
- Atp-dependent rna helicase dhx8 (Complex from Homo sapiens)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein e (9 kDa, Protein from Homo sapiens)
- Protein fam32a (7 kDa, Protein from Homo sapiens)
- Ligated exons: minx mrna (4 kDa, RNA from Human adenovirus 2)
- Snw domain-containing protein 1 (33 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Intron lariat: minx rna (27 kDa, RNA from Human adenovirus 2)
- Pre-mrna-processing factor 17 (59 kDa, Protein from Homo sapiens)
- Pleiotropic regulator 1 (35 kDa, Protein from Homo sapiens)
- Inositol hexakisphosphate (660 Da, Ligand)
- Pre-mrna-processing factor 19 (55 kDa, Protein from Homo sapiens)
- Replication stress response regulator sde2 (4 kDa, Protein from Homo sapiens)
- Atp-dependent rna helicase dhx8 (139 kDa, Protein from Homo sapiens)
- Serine/arginine repetitive matrix protein 2 (2 kDa, Protein from Homo sapiens)
- Potassium ion (39 Da, Ligand)
- Pre-mrna-splicing factor slu7 (68 kDa, Protein from Homo sapiens)
- 116 kda u5 small nuclear ribonucleoprotein component (101 kDa, Protein from Homo sapiens)
- Crooked neck-like protein 1 (100 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein-associated proteins b and b' (9 kDa, Protein from Homo sapiens)
- U6 snrna (34 kDa, RNA from Homo sapiens)
- Pre-mrna-splicing factor syf2 (17 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Pre-mrna-splicing factor rbm22 (33 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Prkr-interacting protein 1 (14 kDa, Protein from Homo sapiens)
- Cell division cycle 5-like protein (92 kDa, Protein from Homo sapiens)
- Eukaryotic initiation factor 4a-iii (44 kDa, Protein from Homo sapiens)
- Cactin (15 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d3 (9 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- U2 snrna (60 kDa, RNA from Homo sapiens)
- Human post-catalytic p complex spliceosome (Complex from Homo sapiens)
- Intron-binding protein aquarius (171 kDa, Protein from Homo sapiens)
- U5 small nuclear ribonucleoprotein 40 kda protein (34 kDa, Protein from Homo sapiens)
- U2 small nuclear ribonucleoprotein b'' (10 kDa, Protein from Homo sapiens)
- Nf-kappa-b-activating protein (3 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein f (8 kDa, Protein from Homo sapiens)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Homo sapiens)
- U5 small nuclear ribonucleoprotein 200 kda helicase (197 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d1 (9 kDa, Protein from Homo sapiens)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Homo sapiens)
- U5 snrna (36 kDa, RNA from Homo sapiens)
- Human post-catalytic p complex spliceosome (3 MDa, Complex)
- Rna-binding protein 8a (10 kDa, Protein from Homo sapiens)
- Protein bud31 homolog (17 kDa, Protein from Homo sapiens)
- Minx introns and exons (Complex from Human adenovirus 2)
- U2 small nuclear ribonucleoprotein a' (18 kDa, Protein from Homo sapiens)
- Protein mago nashi homolog 2 (16 kDa, Protein from Homo sapiens)